Evaluation of the polymerase chain reaction in the diagnosis of cutaneous leishmaniasis due to Leishmania major: a comparison with direct microscopy of smears and sections from lesions
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We have compared the sensitivity of the polymerase chain reaction (PCR) as a diagnostic tool against conventional microscopical diagnostic techniques in patients with cutaneous leishmaniasis from the Sudan. Twenty-eight patients were diagnosed according to clinical criteria followed by microscopical examination of histological sections and slit or impression smears. The PCR had a sensitivity of 86% when used alone, and 93% when combined with Southern blotting. In contrast, microscopy of histological sections had a sensitivity of 76% and slit and impression smears of only 55% and 48%, respectively. The PCR should be considered as a valuable and sensitive diagnostic tool in the diagnosis of cutaneous leishmaniasis; it has the added advantage of identification of the species of Leishmania causing the lesion.
Original language | English |
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Journal | Transactions of the Royal Society of Tropical Medicine and Hygiene |
Volume | 90 |
Issue number | 2 |
Pages (from-to) | 133-5 |
Number of pages | 2 |
ISSN | 0035-9203 |
Publication status | Published - 1996 |
Bibliographical note
Keywords: Animals; Blotting, Southern; Humans; Leishmania major; Leishmaniasis, Cutaneous; Polymerase Chain Reaction; Sensitivity and Specificity; Skin
ID: 6766328