Evaluation of the polymerase chain reaction in the diagnosis of cutaneous leishmaniasis due to Leishmania major: a comparison with direct microscopy of smears and sections from lesions

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Evaluation of the polymerase chain reaction in the diagnosis of cutaneous leishmaniasis due to Leishmania major: a comparison with direct microscopy of smears and sections from lesions. / Andresen, K; Gaafar, A; El-Hassan, A M; Ismail, A; Dafalla, M; Theander, T G; Kharazmi, A.

In: Transactions of the Royal Society of Tropical Medicine and Hygiene, Vol. 90, No. 2, 1996, p. 133-5.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Andresen, K, Gaafar, A, El-Hassan, AM, Ismail, A, Dafalla, M, Theander, TG & Kharazmi, A 1996, 'Evaluation of the polymerase chain reaction in the diagnosis of cutaneous leishmaniasis due to Leishmania major: a comparison with direct microscopy of smears and sections from lesions', Transactions of the Royal Society of Tropical Medicine and Hygiene, vol. 90, no. 2, pp. 133-5.

APA

Andresen, K., Gaafar, A., El-Hassan, A. M., Ismail, A., Dafalla, M., Theander, T. G., & Kharazmi, A. (1996). Evaluation of the polymerase chain reaction in the diagnosis of cutaneous leishmaniasis due to Leishmania major: a comparison with direct microscopy of smears and sections from lesions. Transactions of the Royal Society of Tropical Medicine and Hygiene, 90(2), 133-5.

Vancouver

Andresen K, Gaafar A, El-Hassan AM, Ismail A, Dafalla M, Theander TG et al. Evaluation of the polymerase chain reaction in the diagnosis of cutaneous leishmaniasis due to Leishmania major: a comparison with direct microscopy of smears and sections from lesions. Transactions of the Royal Society of Tropical Medicine and Hygiene. 1996;90(2):133-5.

Author

Andresen, K ; Gaafar, A ; El-Hassan, A M ; Ismail, A ; Dafalla, M ; Theander, T G ; Kharazmi, A. / Evaluation of the polymerase chain reaction in the diagnosis of cutaneous leishmaniasis due to Leishmania major: a comparison with direct microscopy of smears and sections from lesions. In: Transactions of the Royal Society of Tropical Medicine and Hygiene. 1996 ; Vol. 90, No. 2. pp. 133-5.

Bibtex

@article{a0b448e0a0d911dd86a6000ea68e967b,
title = "Evaluation of the polymerase chain reaction in the diagnosis of cutaneous leishmaniasis due to Leishmania major: a comparison with direct microscopy of smears and sections from lesions",
abstract = "We have compared the sensitivity of the polymerase chain reaction (PCR) as a diagnostic tool against conventional microscopical diagnostic techniques in patients with cutaneous leishmaniasis from the Sudan. Twenty-eight patients were diagnosed according to clinical criteria followed by microscopical examination of histological sections and slit or impression smears. The PCR had a sensitivity of 86% when used alone, and 93% when combined with Southern blotting. In contrast, microscopy of histological sections had a sensitivity of 76% and slit and impression smears of only 55% and 48%, respectively. The PCR should be considered as a valuable and sensitive diagnostic tool in the diagnosis of cutaneous leishmaniasis; it has the added advantage of identification of the species of Leishmania causing the lesion.",
author = "K Andresen and A Gaafar and El-Hassan, {A M} and A Ismail and M Dafalla and Theander, {T G} and A Kharazmi",
note = "Keywords: Animals; Blotting, Southern; Humans; Leishmania major; Leishmaniasis, Cutaneous; Polymerase Chain Reaction; Sensitivity and Specificity; Skin",
year = "1996",
language = "English",
volume = "90",
pages = "133--5",
journal = "Transactions of the Royal Society of Tropical Medicine and Hygiene",
issn = "0035-9203",
publisher = "Oxford University Press",
number = "2",

}

RIS

TY - JOUR

T1 - Evaluation of the polymerase chain reaction in the diagnosis of cutaneous leishmaniasis due to Leishmania major: a comparison with direct microscopy of smears and sections from lesions

AU - Andresen, K

AU - Gaafar, A

AU - El-Hassan, A M

AU - Ismail, A

AU - Dafalla, M

AU - Theander, T G

AU - Kharazmi, A

N1 - Keywords: Animals; Blotting, Southern; Humans; Leishmania major; Leishmaniasis, Cutaneous; Polymerase Chain Reaction; Sensitivity and Specificity; Skin

PY - 1996

Y1 - 1996

N2 - We have compared the sensitivity of the polymerase chain reaction (PCR) as a diagnostic tool against conventional microscopical diagnostic techniques in patients with cutaneous leishmaniasis from the Sudan. Twenty-eight patients were diagnosed according to clinical criteria followed by microscopical examination of histological sections and slit or impression smears. The PCR had a sensitivity of 86% when used alone, and 93% when combined with Southern blotting. In contrast, microscopy of histological sections had a sensitivity of 76% and slit and impression smears of only 55% and 48%, respectively. The PCR should be considered as a valuable and sensitive diagnostic tool in the diagnosis of cutaneous leishmaniasis; it has the added advantage of identification of the species of Leishmania causing the lesion.

AB - We have compared the sensitivity of the polymerase chain reaction (PCR) as a diagnostic tool against conventional microscopical diagnostic techniques in patients with cutaneous leishmaniasis from the Sudan. Twenty-eight patients were diagnosed according to clinical criteria followed by microscopical examination of histological sections and slit or impression smears. The PCR had a sensitivity of 86% when used alone, and 93% when combined with Southern blotting. In contrast, microscopy of histological sections had a sensitivity of 76% and slit and impression smears of only 55% and 48%, respectively. The PCR should be considered as a valuable and sensitive diagnostic tool in the diagnosis of cutaneous leishmaniasis; it has the added advantage of identification of the species of Leishmania causing the lesion.

M3 - Journal article

C2 - 8761571

VL - 90

SP - 133

EP - 135

JO - Transactions of the Royal Society of Tropical Medicine and Hygiene

JF - Transactions of the Royal Society of Tropical Medicine and Hygiene

SN - 0035-9203

IS - 2

ER -

ID: 6766328