Analysis by flow cytometry of α2-macroglobulin and nonimmune IgM-binding to Plasmodium falciparum-infected erythrocytes
Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review
Several members of the Plasmodium falciparum erythrocyte membrane protein 1 (PfEMP1) family can bind human serum proteins such as IgM and α2-macroglobulin (α2M). This binding seems to play a role in pathogenesis and immune evasion by improving the avidity of PfEMP1-mediated binding to erythrocyte receptors and/or by masking antibody epitopes in PfEMP1. In this protocol, we describe a flow cytometry-based protocol to evaluate IgM- and α2M-binding to intact and unfixed mature-stage IEs. The method can be used for laboratory clones and field isolates.
| Original language | English |
|---|---|
| Title of host publication | Malaria Immunology : Targeting the Surface of Infected Erythrocytes |
| Number of pages | 10 |
| Volume | 2470 |
| Publisher | Humana Press |
| Publication date | 2022 |
| Pages | 435-444 |
| ISBN (Print) | 978-1-0716-2188-2 |
| ISBN (Electronic) | 978-1-0716-2189-9 |
| DOIs | |
| Publication status | Published - 2022 |
| Series | Methods in molecular biology (Clifton, N.J.) |
|---|---|
| ISSN | 1064-3745 |
Bibliographical note
© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.
- Antibodies, Protozoan, Erythrocytes/metabolism, Female, Flow Cytometry, Humans, Immunoglobulin M, Malaria, Falciparum, Plasmodium falciparum/metabolism, Pregnancy, Pregnancy-Associated alpha 2-Macroglobulins/metabolism, Protozoan Proteins/metabolism
Research areas
ID: 317092512