Analysis by flow cytometry of α2-macroglobulin and nonimmune IgM-binding to Plasmodium falciparum-infected erythrocytes

Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review

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Analysis by flow cytometry of α2-macroglobulin and nonimmune IgM-binding to Plasmodium falciparum-infected erythrocytes. / Hviid, Lars; Lopez-Perez, Mary.

Malaria Immunology: Targeting the Surface of Infected Erythrocytes. Vol. 2470 Humana Press, 2022. p. 435-444 (Methods in molecular biology (Clifton, N.J.)).

Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review

Harvard

Hviid, L & Lopez-Perez, M 2022, Analysis by flow cytometry of α2-macroglobulin and nonimmune IgM-binding to Plasmodium falciparum-infected erythrocytes. in Malaria Immunology: Targeting the Surface of Infected Erythrocytes. vol. 2470, Humana Press, Methods in molecular biology (Clifton, N.J.), pp. 435-444. https://doi.org/10.1007/978-1-0716-2189-9_32

APA

Hviid, L., & Lopez-Perez, M. (2022). Analysis by flow cytometry of α2-macroglobulin and nonimmune IgM-binding to Plasmodium falciparum-infected erythrocytes. In Malaria Immunology: Targeting the Surface of Infected Erythrocytes (Vol. 2470, pp. 435-444). Humana Press. Methods in molecular biology (Clifton, N.J.) https://doi.org/10.1007/978-1-0716-2189-9_32

Vancouver

Hviid L, Lopez-Perez M. Analysis by flow cytometry of α2-macroglobulin and nonimmune IgM-binding to Plasmodium falciparum-infected erythrocytes. In Malaria Immunology: Targeting the Surface of Infected Erythrocytes. Vol. 2470. Humana Press. 2022. p. 435-444. (Methods in molecular biology (Clifton, N.J.)). https://doi.org/10.1007/978-1-0716-2189-9_32

Author

Hviid, Lars ; Lopez-Perez, Mary. / Analysis by flow cytometry of α2-macroglobulin and nonimmune IgM-binding to Plasmodium falciparum-infected erythrocytes. Malaria Immunology: Targeting the Surface of Infected Erythrocytes. Vol. 2470 Humana Press, 2022. pp. 435-444 (Methods in molecular biology (Clifton, N.J.)).

Bibtex

@inbook{f44e59fbbbb943f19f21901abf1458e0,
title = "Analysis by flow cytometry of α2-macroglobulin and nonimmune IgM-binding to Plasmodium falciparum-infected erythrocytes",
abstract = "Several members of the Plasmodium falciparum erythrocyte membrane protein 1 (PfEMP1) family can bind human serum proteins such as IgM and α2-macroglobulin (α2M). This binding seems to play a role in pathogenesis and immune evasion by improving the avidity of PfEMP1-mediated binding to erythrocyte receptors and/or by masking antibody epitopes in PfEMP1. In this protocol, we describe a flow cytometry-based protocol to evaluate IgM- and α2M-binding to intact and unfixed mature-stage IEs. The method can be used for laboratory clones and field isolates.",
keywords = "Antibodies, Protozoan, Erythrocytes/metabolism, Female, Flow Cytometry, Humans, Immunoglobulin M, Malaria, Falciparum, Plasmodium falciparum/metabolism, Pregnancy, Pregnancy-Associated alpha 2-Macroglobulins/metabolism, Protozoan Proteins/metabolism",
author = "Lars Hviid and Mary Lopez-Perez",
note = "{\textcopyright} 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2022",
doi = "10.1007/978-1-0716-2189-9_32",
language = "English",
isbn = "978-1-0716-2188-2",
volume = "2470",
series = "Methods in molecular biology (Clifton, N.J.)",
publisher = "Humana Press",
pages = "435--444",
booktitle = "Malaria Immunology",
address = "United States",

}

RIS

TY - CHAP

T1 - Analysis by flow cytometry of α2-macroglobulin and nonimmune IgM-binding to Plasmodium falciparum-infected erythrocytes

AU - Hviid, Lars

AU - Lopez-Perez, Mary

N1 - © 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

PY - 2022

Y1 - 2022

N2 - Several members of the Plasmodium falciparum erythrocyte membrane protein 1 (PfEMP1) family can bind human serum proteins such as IgM and α2-macroglobulin (α2M). This binding seems to play a role in pathogenesis and immune evasion by improving the avidity of PfEMP1-mediated binding to erythrocyte receptors and/or by masking antibody epitopes in PfEMP1. In this protocol, we describe a flow cytometry-based protocol to evaluate IgM- and α2M-binding to intact and unfixed mature-stage IEs. The method can be used for laboratory clones and field isolates.

AB - Several members of the Plasmodium falciparum erythrocyte membrane protein 1 (PfEMP1) family can bind human serum proteins such as IgM and α2-macroglobulin (α2M). This binding seems to play a role in pathogenesis and immune evasion by improving the avidity of PfEMP1-mediated binding to erythrocyte receptors and/or by masking antibody epitopes in PfEMP1. In this protocol, we describe a flow cytometry-based protocol to evaluate IgM- and α2M-binding to intact and unfixed mature-stage IEs. The method can be used for laboratory clones and field isolates.

KW - Antibodies, Protozoan

KW - Erythrocytes/metabolism

KW - Female

KW - Flow Cytometry

KW - Humans

KW - Immunoglobulin M

KW - Malaria, Falciparum

KW - Plasmodium falciparum/metabolism

KW - Pregnancy

KW - Pregnancy-Associated alpha 2-Macroglobulins/metabolism

KW - Protozoan Proteins/metabolism

U2 - 10.1007/978-1-0716-2189-9_32

DO - 10.1007/978-1-0716-2189-9_32

M3 - Book chapter

C2 - 35881364

SN - 978-1-0716-2188-2

VL - 2470

T3 - Methods in molecular biology (Clifton, N.J.)

SP - 435

EP - 444

BT - Malaria Immunology

PB - Humana Press

ER -

ID: 317092512