A versatile, high through-put, bead-based phagocytosis assay for Plasmodium falciparum
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- A versatile, high through-put, bead-based phagocytosis assay for Plasmodium falciparum
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Antibody-mediated phagocytosis is an important immune effector mechanism against Plasmodium falciparum-infected erythrocytes (IE); however, current phagocytosis assays use IE collected from infected individuals or from in vitro cultures of P. falciparum, making them prone to high variation. A simple, high-throughput flow cytometric assay was developed that uses THP-1 cells and fluorescent beads covalently-coupled with the malarial antigen VAR2CSA. The assay is highly repeatable, provides both the overall percent phagocytosis and semi-quantitates the number of antigen-coupled beads internalized.
Original language | English |
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Article number | 14705 |
Journal | Scientific Reports |
Volume | 7 |
Number of pages | 7 |
ISSN | 2045-2322 |
DOIs | |
Publication status | Published - 2017 |
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