A versatile, high through-put, bead-based phagocytosis assay for Plasmodium falciparum

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A versatile, high through-put, bead-based phagocytosis assay for Plasmodium falciparum. / Lloyd, Yukie M.; Ngati, Elise P.; Salanti, Ali; Leke, Rose G.F.; Taylor, Diane W.

In: Scientific Reports, Vol. 7, 14705, 2017.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Lloyd, YM, Ngati, EP, Salanti, A, Leke, RGF & Taylor, DW 2017, 'A versatile, high through-put, bead-based phagocytosis assay for Plasmodium falciparum', Scientific Reports, vol. 7, 14705. https://doi.org/10.1038/s41598-017-13900-4

APA

Lloyd, Y. M., Ngati, E. P., Salanti, A., Leke, R. G. F., & Taylor, D. W. (2017). A versatile, high through-put, bead-based phagocytosis assay for Plasmodium falciparum. Scientific Reports, 7, [14705]. https://doi.org/10.1038/s41598-017-13900-4

Vancouver

Lloyd YM, Ngati EP, Salanti A, Leke RGF, Taylor DW. A versatile, high through-put, bead-based phagocytosis assay for Plasmodium falciparum. Scientific Reports. 2017;7. 14705. https://doi.org/10.1038/s41598-017-13900-4

Author

Lloyd, Yukie M. ; Ngati, Elise P. ; Salanti, Ali ; Leke, Rose G.F. ; Taylor, Diane W. / A versatile, high through-put, bead-based phagocytosis assay for Plasmodium falciparum. In: Scientific Reports. 2017 ; Vol. 7.

Bibtex

@article{1e41d46708ea4a1b8273af9f179eeb81,
title = "A versatile, high through-put, bead-based phagocytosis assay for Plasmodium falciparum",
abstract = "Antibody-mediated phagocytosis is an important immune effector mechanism against Plasmodium falciparum-infected erythrocytes (IE); however, current phagocytosis assays use IE collected from infected individuals or from in vitro cultures of P. falciparum, making them prone to high variation. A simple, high-throughput flow cytometric assay was developed that uses THP-1 cells and fluorescent beads covalently-coupled with the malarial antigen VAR2CSA. The assay is highly repeatable, provides both the overall percent phagocytosis and semi-quantitates the number of antigen-coupled beads internalized.",
author = "Lloyd, {Yukie M.} and Ngati, {Elise P.} and Ali Salanti and Leke, {Rose G.F.} and Taylor, {Diane W.}",
year = "2017",
doi = "10.1038/s41598-017-13900-4",
language = "English",
volume = "7",
journal = "Scientific Reports",
issn = "2045-2322",
publisher = "nature publishing group",

}

RIS

TY - JOUR

T1 - A versatile, high through-put, bead-based phagocytosis assay for Plasmodium falciparum

AU - Lloyd, Yukie M.

AU - Ngati, Elise P.

AU - Salanti, Ali

AU - Leke, Rose G.F.

AU - Taylor, Diane W.

PY - 2017

Y1 - 2017

N2 - Antibody-mediated phagocytosis is an important immune effector mechanism against Plasmodium falciparum-infected erythrocytes (IE); however, current phagocytosis assays use IE collected from infected individuals or from in vitro cultures of P. falciparum, making them prone to high variation. A simple, high-throughput flow cytometric assay was developed that uses THP-1 cells and fluorescent beads covalently-coupled with the malarial antigen VAR2CSA. The assay is highly repeatable, provides both the overall percent phagocytosis and semi-quantitates the number of antigen-coupled beads internalized.

AB - Antibody-mediated phagocytosis is an important immune effector mechanism against Plasmodium falciparum-infected erythrocytes (IE); however, current phagocytosis assays use IE collected from infected individuals or from in vitro cultures of P. falciparum, making them prone to high variation. A simple, high-throughput flow cytometric assay was developed that uses THP-1 cells and fluorescent beads covalently-coupled with the malarial antigen VAR2CSA. The assay is highly repeatable, provides both the overall percent phagocytosis and semi-quantitates the number of antigen-coupled beads internalized.

U2 - 10.1038/s41598-017-13900-4

DO - 10.1038/s41598-017-13900-4

M3 - Journal article

C2 - 29089635

AN - SCOPUS:85032685615

VL - 7

JO - Scientific Reports

JF - Scientific Reports

SN - 2045-2322

M1 - 14705

ER -

ID: 185722180