Limited cross-reactivity among domains of the Plasmodium falciparum clone 3D7 erythrocyte membrane protein 1 family

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Limited cross-reactivity among domains of the Plasmodium falciparum clone 3D7 erythrocyte membrane protein 1 family. / Joergensen, Louise; Turner, Louise; Magistrado, Pamela; Dahlbäck, Madeleine A; Vestergaard, Lasse S; Lusingu, John P; Lemnge, Martha; Salanti, Ali; Theander, Thor G; Jensen, Anja T R.

In: Infection and Immunity, Vol. 74, No. 12, 2006, p. 6778-84.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Joergensen, L, Turner, L, Magistrado, P, Dahlbäck, MA, Vestergaard, LS, Lusingu, JP, Lemnge, M, Salanti, A, Theander, TG & Jensen, ATR 2006, 'Limited cross-reactivity among domains of the Plasmodium falciparum clone 3D7 erythrocyte membrane protein 1 family', Infection and Immunity, vol. 74, no. 12, pp. 6778-84. https://doi.org/10.1128/IAI.01187-06

APA

Joergensen, L., Turner, L., Magistrado, P., Dahlbäck, M. A., Vestergaard, L. S., Lusingu, J. P., Lemnge, M., Salanti, A., Theander, T. G., & Jensen, A. T. R. (2006). Limited cross-reactivity among domains of the Plasmodium falciparum clone 3D7 erythrocyte membrane protein 1 family. Infection and Immunity, 74(12), 6778-84. https://doi.org/10.1128/IAI.01187-06

Vancouver

Joergensen L, Turner L, Magistrado P, Dahlbäck MA, Vestergaard LS, Lusingu JP et al. Limited cross-reactivity among domains of the Plasmodium falciparum clone 3D7 erythrocyte membrane protein 1 family. Infection and Immunity. 2006;74(12):6778-84. https://doi.org/10.1128/IAI.01187-06

Author

Joergensen, Louise ; Turner, Louise ; Magistrado, Pamela ; Dahlbäck, Madeleine A ; Vestergaard, Lasse S ; Lusingu, John P ; Lemnge, Martha ; Salanti, Ali ; Theander, Thor G ; Jensen, Anja T R. / Limited cross-reactivity among domains of the Plasmodium falciparum clone 3D7 erythrocyte membrane protein 1 family. In: Infection and Immunity. 2006 ; Vol. 74, No. 12. pp. 6778-84.

Bibtex

@article{17038340a0d411dd86a6000ea68e967b,
title = "Limited cross-reactivity among domains of the Plasmodium falciparum clone 3D7 erythrocyte membrane protein 1 family",
abstract = "The var gene-encoded Plasmodium falciparum erythrocyte membrane protein 1 (PfEMP1) family is responsible for antigenic variation and sequestration of infected erythrocytes during malaria. We have previously grouped the 60 PfEMP1 variants of P. falciparum clone 3D7 into groups A and B/A (category A) and groups B, B/C, and C (category non-A). Expression of category A molecules is associated with severe malaria, and that of category non-A molecules is associated with uncomplicated malaria and asymptomatic infection. Here we assessed cross-reactivity among 60 different recombinant PfEMP1 domains derived from clone 3D7 by using a competition enzyme-linked immunosorbent assay and a pool of plasma from 63 malaria-exposed Tanzanian individuals. We conclude that naturally acquired antibodies are largely directed toward epitopes varying between different domains with a few, mainly category A, domains sharing cross-reactive antibody epitopes. Identification of groups of serological cross-reacting molecules is pivotal for the development of vaccines based on PfEMP1.",
author = "Louise Joergensen and Louise Turner and Pamela Magistrado and Dahlb{\"a}ck, {Madeleine A} and Vestergaard, {Lasse S} and Lusingu, {John P} and Martha Lemnge and Ali Salanti and Theander, {Thor G} and Jensen, {Anja T R}",
note = "Keywords: Animals; Antibodies, Protozoan; Binding, Competitive; Cross Reactions; Enzyme-Linked Immunosorbent Assay; Humans; Immunodominant Epitopes; Malaria Vaccines; Malaria, Falciparum; Plasmodium falciparum; Protein Structure, Tertiary; Protozoan Proteins; Recombinant Proteins",
year = "2006",
doi = "10.1128/IAI.01187-06",
language = "English",
volume = "74",
pages = "6778--84",
journal = "Infection and Immunity",
issn = "0019-9567",
publisher = "American Society for Microbiology",
number = "12",

}

RIS

TY - JOUR

T1 - Limited cross-reactivity among domains of the Plasmodium falciparum clone 3D7 erythrocyte membrane protein 1 family

AU - Joergensen, Louise

AU - Turner, Louise

AU - Magistrado, Pamela

AU - Dahlbäck, Madeleine A

AU - Vestergaard, Lasse S

AU - Lusingu, John P

AU - Lemnge, Martha

AU - Salanti, Ali

AU - Theander, Thor G

AU - Jensen, Anja T R

N1 - Keywords: Animals; Antibodies, Protozoan; Binding, Competitive; Cross Reactions; Enzyme-Linked Immunosorbent Assay; Humans; Immunodominant Epitopes; Malaria Vaccines; Malaria, Falciparum; Plasmodium falciparum; Protein Structure, Tertiary; Protozoan Proteins; Recombinant Proteins

PY - 2006

Y1 - 2006

N2 - The var gene-encoded Plasmodium falciparum erythrocyte membrane protein 1 (PfEMP1) family is responsible for antigenic variation and sequestration of infected erythrocytes during malaria. We have previously grouped the 60 PfEMP1 variants of P. falciparum clone 3D7 into groups A and B/A (category A) and groups B, B/C, and C (category non-A). Expression of category A molecules is associated with severe malaria, and that of category non-A molecules is associated with uncomplicated malaria and asymptomatic infection. Here we assessed cross-reactivity among 60 different recombinant PfEMP1 domains derived from clone 3D7 by using a competition enzyme-linked immunosorbent assay and a pool of plasma from 63 malaria-exposed Tanzanian individuals. We conclude that naturally acquired antibodies are largely directed toward epitopes varying between different domains with a few, mainly category A, domains sharing cross-reactive antibody epitopes. Identification of groups of serological cross-reacting molecules is pivotal for the development of vaccines based on PfEMP1.

AB - The var gene-encoded Plasmodium falciparum erythrocyte membrane protein 1 (PfEMP1) family is responsible for antigenic variation and sequestration of infected erythrocytes during malaria. We have previously grouped the 60 PfEMP1 variants of P. falciparum clone 3D7 into groups A and B/A (category A) and groups B, B/C, and C (category non-A). Expression of category A molecules is associated with severe malaria, and that of category non-A molecules is associated with uncomplicated malaria and asymptomatic infection. Here we assessed cross-reactivity among 60 different recombinant PfEMP1 domains derived from clone 3D7 by using a competition enzyme-linked immunosorbent assay and a pool of plasma from 63 malaria-exposed Tanzanian individuals. We conclude that naturally acquired antibodies are largely directed toward epitopes varying between different domains with a few, mainly category A, domains sharing cross-reactive antibody epitopes. Identification of groups of serological cross-reacting molecules is pivotal for the development of vaccines based on PfEMP1.

U2 - 10.1128/IAI.01187-06

DO - 10.1128/IAI.01187-06

M3 - Journal article

C2 - 17015460

VL - 74

SP - 6778

EP - 6784

JO - Infection and Immunity

JF - Infection and Immunity

SN - 0019-9567

IS - 12

ER -

ID: 6765087