Interferon-¿ and interleukin-4 production by human T cells recognizing Leishmania donovani antigens separated by SDS-PAGE
Research output: Contribution to journal › Journal article › Research › peer-review
Crude preparations of Leishmania donovani proteins were separated by preparative SDS-polyacrylamide gel electrophoresis. Fractions of separated proteins were recovered by electroelution directly from the gel into separate chambers. The isolated protein fractions were tested for induction of proliferation and interferon-gamma (IFN-gamma) production in cultures of peripheral blood mononuclear cells (PBMC) from individuals who had recovered from visceral leishmaniasis caused by L. donovani. The release of interleukin-4 (IL-4) by PBMC stimulated with the isolated L. donovani antigen fractions was measured after treatment with phorbol-myristate-acetate and ionomycin. The cells proliferated in response to all protein fractions with molecular weights in the range < 12 kDa to 85 kDa. In general, IFN-gamma was secreted in response to stimulation with all the protein fractions, whereas IL-4 production was infrequently observed. The results show that T cells from individuals who have been cured of visceral leishmaniasis recognize and respond to a wide range of leishmanial antigens. There was no evidence of particular fractions constantly giving either IFN-gamma or IL-4-producing responses.
Original language | English |
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Journal | Acta Pathologica Microbiologica et Immunologica Scandinavica |
Volume | 103 |
Issue number | 2 |
Pages (from-to) | 131-9 |
Number of pages | 8 |
ISSN | 0903-4641 |
Publication status | Published - 1995 |
Bibliographical note
Keywords: Animals; Antigens, Protozoan; Cells, Cultured; Cytokines; Electrophoresis, Polyacrylamide Gel; Humans; Interferon Type II; Interleukin-4; Leishmania donovani; Leishmaniasis, Visceral; Lymphocyte Activation; T-Lymphocytes
ID: 6766502