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Comparative analysis of spike-specific IgG Fc glycoprofiles elicited by adenoviral, mRNA, and protein-based SARS-CoV-2 vaccines. / Van Coillie, Julie; Pongracz, Tamas; Šuštić, Tonći; Wang, Wenjun; Nouta, Jan; Le Gars, Mathieu; Keijzer, Sofie; Linty, Federica; Cristianawati, Olvi; Keijser, Jim B.D.; Visser, Remco; van Vught, Lonneke A.; Slim, Marleen A.; van Mourik, Niels; Smit, Merel J.; Sander, Adam; Schmidt, David E.; Steenhuis, Maurice; Rispens, Theo; Nielsen, Morten A.; Mordmüller, Benjamin G.; Vlaar, Alexander P.J.; Ellen van der Schoot, C.; Roozendaal, Ramon; Wuhrer, Manfred; Vidarsson, Gestur; Appelman, Brent; van de Beek, Diederik; Bomers, Marije K.; de Brabander, Justin; Brouwer, Matthijs C.; Buis, David T.P.; Chekrouni, Nora; van Gils, Marit J.; de Jong, Menno D.; Lavell, Ayesha H.A.; Olie, Sabine E.; Peters, Edgar J.G.; Reijnders, Tom D.Y.; Schinkel, Michiel; Schuurman, Alex R.; Sikkens, Jonne J.; Smulders, Yvo M.; Wiersinga, Joost W.; Spinello, Antinori; Bassoli, Cinzia; Bestetti, Giovanna; Corbellino, Mario; Salanti, Ali; Theander, Thor G.; in collaboration with the UMC COVID-19 S3/HCW study group.
In:
iScience, Vol. 26, No. 9, 107619, 2023, p. 1-15.
Research output: Contribution to journal › Journal article › Research › peer-review
Harvard
Van Coillie, J, Pongracz, T, Šuštić, T, Wang, W, Nouta, J, Le Gars, M, Keijzer, S, Linty, F, Cristianawati, O, Keijser, JBD, Visser, R, van Vught, LA, Slim, MA, van Mourik, N, Smit, MJ
, Sander, A, Schmidt, DE, Steenhuis, M, Rispens, T
, Nielsen, MA, Mordmüller, BG, Vlaar, APJ, Ellen van der Schoot, C, Roozendaal, R, Wuhrer, M, Vidarsson, G, Appelman, B, van de Beek, D, Bomers, MK, de Brabander, J, Brouwer, MC, Buis, DTP, Chekrouni, N, van Gils, MJ, de Jong, MD, Lavell, AHA, Olie, SE, Peters, EJG, Reijnders, TDY, Schinkel, M, Schuurman, AR, Sikkens, JJ, Smulders, YM, Wiersinga, JW, Spinello, A, Bassoli, C, Bestetti, G, Corbellino, M
, Salanti, A, Theander, TG & in collaboration with the UMC COVID-19 S3/HCW study group 2023, '
Comparative analysis of spike-specific IgG Fc glycoprofiles elicited by adenoviral, mRNA, and protein-based SARS-CoV-2 vaccines',
iScience, vol. 26, no. 9, 107619, pp. 1-15.
https://doi.org/10.1016/j.isci.2023.107619APA
Van Coillie, J., Pongracz, T., Šuštić, T., Wang, W., Nouta, J., Le Gars, M., Keijzer, S., Linty, F., Cristianawati, O., Keijser, J. B. D., Visser, R., van Vught, L. A., Slim, M. A., van Mourik, N., Smit, M. J.
, Sander, A., Schmidt, D. E., Steenhuis, M., Rispens, T., ... in collaboration with the UMC COVID-19 S3/HCW study group (2023).
Comparative analysis of spike-specific IgG Fc glycoprofiles elicited by adenoviral, mRNA, and protein-based SARS-CoV-2 vaccines.
iScience,
26(9), 1-15. [107619].
https://doi.org/10.1016/j.isci.2023.107619Vancouver
Van Coillie J, Pongracz T, Šuštić T, Wang W, Nouta J, Le Gars M et al.
Comparative analysis of spike-specific IgG Fc glycoprofiles elicited by adenoviral, mRNA, and protein-based SARS-CoV-2 vaccines.
iScience. 2023;26(9):1-15. 107619.
https://doi.org/10.1016/j.isci.2023.107619Author
Van Coillie, Julie ; Pongracz, Tamas ; Šuštić, Tonći ; Wang, Wenjun ; Nouta, Jan ; Le Gars, Mathieu ; Keijzer, Sofie ; Linty, Federica ; Cristianawati, Olvi ; Keijser, Jim B.D. ; Visser, Remco ; van Vught, Lonneke A. ; Slim, Marleen A. ; van Mourik, Niels ; Smit, Merel J. ; Sander, Adam ; Schmidt, David E. ; Steenhuis, Maurice ; Rispens, Theo ; Nielsen, Morten A. ; Mordmüller, Benjamin G. ; Vlaar, Alexander P.J. ; Ellen van der Schoot, C. ; Roozendaal, Ramon ; Wuhrer, Manfred ; Vidarsson, Gestur ; Appelman, Brent ; van de Beek, Diederik ; Bomers, Marije K. ; de Brabander, Justin ; Brouwer, Matthijs C. ; Buis, David T.P. ; Chekrouni, Nora ; van Gils, Marit J. ; de Jong, Menno D. ; Lavell, Ayesha H.A. ; Olie, Sabine E. ; Peters, Edgar J.G. ; Reijnders, Tom D.Y. ; Schinkel, Michiel ; Schuurman, Alex R. ; Sikkens, Jonne J. ; Smulders, Yvo M. ; Wiersinga, Joost W. ; Spinello, Antinori ; Bassoli, Cinzia ; Bestetti, Giovanna ; Corbellino, Mario ; Salanti, Ali ; Theander, Thor G. ; in collaboration with the UMC COVID-19 S3/HCW study group. / Comparative analysis of spike-specific IgG Fc glycoprofiles elicited by adenoviral, mRNA, and protein-based SARS-CoV-2 vaccines. In: iScience. 2023 ; Vol. 26, No. 9. pp. 1-15.
Bibtex
@article{0c785b1867c84262b311f102b7a0b864,
title = "Comparative analysis of spike-specific IgG Fc glycoprofiles elicited by adenoviral, mRNA, and protein-based SARS-CoV-2 vaccines",
abstract = "IgG antibodies are important mediators of vaccine-induced immunity through complement- and Fc receptor-dependent effector functions. Both are influenced by the composition of the conserved N-linked glycan located in the IgG Fc domain. Here, we compared the anti-Spike (S) IgG1 Fc glycosylation profiles in response to mRNA, adenoviral, and protein-based COVID-19 vaccines by mass spectrometry (MS). All vaccines induced a transient increase of antigen-specific IgG1 Fc galactosylation and sialylation. An initial, transient increase of afucosylated IgG was induced by membrane-encoding S protein formulations. A fucose-sensitive ELISA for antigen-specific IgG (FEASI) exploiting FcγRIIIa affinity for afucosylated IgG was used as an orthogonal method to confirm the LC-MS-based afucosylation readout. Our data suggest that vaccine-induced anti-S IgG glycosylation is dynamic, and although variation is seen between different vaccine platforms and individuals, the evolution of glycosylation patterns display marked overlaps.",
keywords = "Glycomics, Immune response, Immunology, Microbiology",
author = "{Van Coillie}, Julie and Tamas Pongracz and Ton{\'c}i {\v S}u{\v s}ti{\'c} and Wenjun Wang and Jan Nouta and {Le Gars}, Mathieu and Sofie Keijzer and Federica Linty and Olvi Cristianawati and Keijser, {Jim B.D.} and Remco Visser and {van Vught}, {Lonneke A.} and Slim, {Marleen A.} and {van Mourik}, Niels and Smit, {Merel J.} and Adam Sander and Schmidt, {David E.} and Maurice Steenhuis and Theo Rispens and Nielsen, {Morten A.} and Mordm{\"u}ller, {Benjamin G.} and Vlaar, {Alexander P.J.} and {Ellen van der Schoot}, C. and Ramon Roozendaal and Manfred Wuhrer and Gestur Vidarsson and Brent Appelman and {van de Beek}, Diederik and Bomers, {Marije K.} and {de Brabander}, Justin and Brouwer, {Matthijs C.} and Buis, {David T.P.} and Nora Chekrouni and {van Gils}, {Marit J.} and {de Jong}, {Menno D.} and Lavell, {Ayesha H.A.} and Olie, {Sabine E.} and Peters, {Edgar J.G.} and Reijnders, {Tom D.Y.} and Michiel Schinkel and Schuurman, {Alex R.} and Sikkens, {Jonne J.} and Smulders, {Yvo M.} and Wiersinga, {Joost W.} and Antinori Spinello and Cinzia Bassoli and Giovanna Bestetti and Mario Corbellino and Ali Salanti and Theander, {Thor G.} and {in collaboration with the UMC COVID-19 S3/HCW study group}",
note = "Publisher Copyright: {\textcopyright} 2023 The Author(s)",
year = "2023",
doi = "10.1016/j.isci.2023.107619",
language = "English",
volume = "26",
pages = "1--15",
journal = "iScience",
issn = "2589-0042",
publisher = "Elsevier",
number = "9",
}
RIS
TY - JOUR
T1 - Comparative analysis of spike-specific IgG Fc glycoprofiles elicited by adenoviral, mRNA, and protein-based SARS-CoV-2 vaccines
AU - Van Coillie, Julie
AU - Pongracz, Tamas
AU - Šuštić, Tonći
AU - Wang, Wenjun
AU - Nouta, Jan
AU - Le Gars, Mathieu
AU - Keijzer, Sofie
AU - Linty, Federica
AU - Cristianawati, Olvi
AU - Keijser, Jim B.D.
AU - Visser, Remco
AU - van Vught, Lonneke A.
AU - Slim, Marleen A.
AU - van Mourik, Niels
AU - Smit, Merel J.
AU - Sander, Adam
AU - Schmidt, David E.
AU - Steenhuis, Maurice
AU - Rispens, Theo
AU - Nielsen, Morten A.
AU - Mordmüller, Benjamin G.
AU - Vlaar, Alexander P.J.
AU - Ellen van der Schoot, C.
AU - Roozendaal, Ramon
AU - Wuhrer, Manfred
AU - Vidarsson, Gestur
AU - Appelman, Brent
AU - van de Beek, Diederik
AU - Bomers, Marije K.
AU - de Brabander, Justin
AU - Brouwer, Matthijs C.
AU - Buis, David T.P.
AU - Chekrouni, Nora
AU - van Gils, Marit J.
AU - de Jong, Menno D.
AU - Lavell, Ayesha H.A.
AU - Olie, Sabine E.
AU - Peters, Edgar J.G.
AU - Reijnders, Tom D.Y.
AU - Schinkel, Michiel
AU - Schuurman, Alex R.
AU - Sikkens, Jonne J.
AU - Smulders, Yvo M.
AU - Wiersinga, Joost W.
AU - Spinello, Antinori
AU - Bassoli, Cinzia
AU - Bestetti, Giovanna
AU - Corbellino, Mario
AU - Salanti, Ali
AU - Theander, Thor G.
AU - in collaboration with the UMC COVID-19 S3/HCW study group
N1 - Publisher Copyright:
© 2023 The Author(s)
PY - 2023
Y1 - 2023
N2 - IgG antibodies are important mediators of vaccine-induced immunity through complement- and Fc receptor-dependent effector functions. Both are influenced by the composition of the conserved N-linked glycan located in the IgG Fc domain. Here, we compared the anti-Spike (S) IgG1 Fc glycosylation profiles in response to mRNA, adenoviral, and protein-based COVID-19 vaccines by mass spectrometry (MS). All vaccines induced a transient increase of antigen-specific IgG1 Fc galactosylation and sialylation. An initial, transient increase of afucosylated IgG was induced by membrane-encoding S protein formulations. A fucose-sensitive ELISA for antigen-specific IgG (FEASI) exploiting FcγRIIIa affinity for afucosylated IgG was used as an orthogonal method to confirm the LC-MS-based afucosylation readout. Our data suggest that vaccine-induced anti-S IgG glycosylation is dynamic, and although variation is seen between different vaccine platforms and individuals, the evolution of glycosylation patterns display marked overlaps.
AB - IgG antibodies are important mediators of vaccine-induced immunity through complement- and Fc receptor-dependent effector functions. Both are influenced by the composition of the conserved N-linked glycan located in the IgG Fc domain. Here, we compared the anti-Spike (S) IgG1 Fc glycosylation profiles in response to mRNA, adenoviral, and protein-based COVID-19 vaccines by mass spectrometry (MS). All vaccines induced a transient increase of antigen-specific IgG1 Fc galactosylation and sialylation. An initial, transient increase of afucosylated IgG was induced by membrane-encoding S protein formulations. A fucose-sensitive ELISA for antigen-specific IgG (FEASI) exploiting FcγRIIIa affinity for afucosylated IgG was used as an orthogonal method to confirm the LC-MS-based afucosylation readout. Our data suggest that vaccine-induced anti-S IgG glycosylation is dynamic, and although variation is seen between different vaccine platforms and individuals, the evolution of glycosylation patterns display marked overlaps.
KW - Glycomics
KW - Immune response
KW - Immunology
KW - Microbiology
U2 - 10.1016/j.isci.2023.107619
DO - 10.1016/j.isci.2023.107619
M3 - Journal article
C2 - 37670790
AN - SCOPUS:85169841393
VL - 26
SP - 1
EP - 15
JO - iScience
JF - iScience
SN - 2589-0042
IS - 9
M1 - 107619
ER -
