Chip-based assay of adhesion of Plasmodium falciparum-infected erythrocytes to cells under flow
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Chip-based assay of adhesion of Plasmodium falciparum-infected erythrocytes to cells under flow. / Adams, Yvonne; Jensen, Anja Ramstedt.
Malaria Immunology: Targeting the Surface of Infected Erythrocytes. ed. / Anja Tatiana Ramstedt Jensen; Lars Hviid. Humana Press, 2022. p. 545-556 (Methods in molecular biology (Clifton, N.J.)).Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review
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TY - CHAP
T1 - Chip-based assay of adhesion of Plasmodium falciparum-infected erythrocytes to cells under flow
AU - Adams, Yvonne
AU - Jensen, Anja Ramstedt
N1 - © 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.
PY - 2022
Y1 - 2022
N2 - Unique to Plasmodium falciparum malaria parasites, the mature asexual stages of the life cycle are absent from the peripheral blood stream. Using syringe pumps and commercially available microslides, it is possible to mimic the blood flow, and investigate the interactions of erythrocytes infected by well-defined P. falciparum isolates for their ability to bind to various tissue receptors under physiological flow conditions. This chapter outlines the techniques needed to investigate how parasites bind to endothelial cells under physiological sheer stress conditions.
AB - Unique to Plasmodium falciparum malaria parasites, the mature asexual stages of the life cycle are absent from the peripheral blood stream. Using syringe pumps and commercially available microslides, it is possible to mimic the blood flow, and investigate the interactions of erythrocytes infected by well-defined P. falciparum isolates for their ability to bind to various tissue receptors under physiological flow conditions. This chapter outlines the techniques needed to investigate how parasites bind to endothelial cells under physiological sheer stress conditions.
KW - Cell Adhesion/physiology
KW - Endothelial Cells
KW - Erythrocytes/parasitology
KW - Humans
KW - Malaria, Falciparum/parasitology
KW - Plasmodium falciparum/physiology
U2 - 10.1007/978-1-0716-2189-9_42
DO - 10.1007/978-1-0716-2189-9_42
M3 - Book chapter
C2 - 35881374
T3 - Methods in molecular biology (Clifton, N.J.)
SP - 545
EP - 556
BT - Malaria Immunology
A2 - Jensen, Anja Tatiana Ramstedt
A2 - Hviid, Lars
PB - Humana Press
ER -
ID: 326848678