Baculovirus-expressed constructs induce immunoglobulin G that recognizes VAR2CSA on Plasmodium falciparum-infected erythrocytes

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Baculovirus-expressed constructs induce immunoglobulin G that recognizes VAR2CSA on Plasmodium falciparum-infected erythrocytes. / Barfod, Lea; Nielsen, Morten A; Turner, Louise; Dahlbäck, Madeleine; Jensen, Anja T R; Hviid, Lars; Theander, Thor G; Salanti, Ali.

In: Infection and Immunity, Vol. 74, No. 7, 2006, p. 4357-60.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Barfod, L, Nielsen, MA, Turner, L, Dahlbäck, M, Jensen, ATR, Hviid, L, Theander, TG & Salanti, A 2006, 'Baculovirus-expressed constructs induce immunoglobulin G that recognizes VAR2CSA on Plasmodium falciparum-infected erythrocytes', Infection and Immunity, vol. 74, no. 7, pp. 4357-60. https://doi.org/10.1128/IAI.01617-05

APA

Barfod, L., Nielsen, M. A., Turner, L., Dahlbäck, M., Jensen, A. T. R., Hviid, L., Theander, T. G., & Salanti, A. (2006). Baculovirus-expressed constructs induce immunoglobulin G that recognizes VAR2CSA on Plasmodium falciparum-infected erythrocytes. Infection and Immunity, 74(7), 4357-60. https://doi.org/10.1128/IAI.01617-05

Vancouver

Barfod L, Nielsen MA, Turner L, Dahlbäck M, Jensen ATR, Hviid L et al. Baculovirus-expressed constructs induce immunoglobulin G that recognizes VAR2CSA on Plasmodium falciparum-infected erythrocytes. Infection and Immunity. 2006;74(7):4357-60. https://doi.org/10.1128/IAI.01617-05

Author

Barfod, Lea ; Nielsen, Morten A ; Turner, Louise ; Dahlbäck, Madeleine ; Jensen, Anja T R ; Hviid, Lars ; Theander, Thor G ; Salanti, Ali. / Baculovirus-expressed constructs induce immunoglobulin G that recognizes VAR2CSA on Plasmodium falciparum-infected erythrocytes. In: Infection and Immunity. 2006 ; Vol. 74, No. 7. pp. 4357-60.

Bibtex

@article{8511e2d0a02c11dd86a6000ea68e967b,
title = "Baculovirus-expressed constructs induce immunoglobulin G that recognizes VAR2CSA on Plasmodium falciparum-infected erythrocytes",
abstract = "We raised specific antisera against recombinant VAR2CSA domains produced in Escherichia coli and in insect cells. All were reactive in enzyme-linked immunosorbent assay, but only insect cell-derived constructs induced immunoglobulin G (IgG) that was reactive with native VAR2CSA on the surface of infected erythrocytes. Our data show that five of the six VAR2CSA Duffy-binding-like domains are surface exposed and that induction of surface-reactive VAR2CSA-specific IgG depends critically upon antigen conformation. These findings have implications for the development of vaccines against pregnancy-associated Plasmodium falciparum malaria.",
author = "Lea Barfod and Nielsen, {Morten A} and Louise Turner and Madeleine Dahlb{\"a}ck and Jensen, {Anja T R} and Lars Hviid and Theander, {Thor G} and Ali Salanti",
note = "Keywords: Animals; Antigens, Protozoan; Baculoviridae; Erythrocytes; Female; Genetic Vectors; Humans; Immune Sera; Immunoglobulin G; Mice; Plasmodium falciparum; Rabbits; Recombinant Proteins",
year = "2006",
doi = "10.1128/IAI.01617-05",
language = "English",
volume = "74",
pages = "4357--60",
journal = "Infection and Immunity",
issn = "0019-9567",
publisher = "American Society for Microbiology",
number = "7",

}

RIS

TY - JOUR

T1 - Baculovirus-expressed constructs induce immunoglobulin G that recognizes VAR2CSA on Plasmodium falciparum-infected erythrocytes

AU - Barfod, Lea

AU - Nielsen, Morten A

AU - Turner, Louise

AU - Dahlbäck, Madeleine

AU - Jensen, Anja T R

AU - Hviid, Lars

AU - Theander, Thor G

AU - Salanti, Ali

N1 - Keywords: Animals; Antigens, Protozoan; Baculoviridae; Erythrocytes; Female; Genetic Vectors; Humans; Immune Sera; Immunoglobulin G; Mice; Plasmodium falciparum; Rabbits; Recombinant Proteins

PY - 2006

Y1 - 2006

N2 - We raised specific antisera against recombinant VAR2CSA domains produced in Escherichia coli and in insect cells. All were reactive in enzyme-linked immunosorbent assay, but only insect cell-derived constructs induced immunoglobulin G (IgG) that was reactive with native VAR2CSA on the surface of infected erythrocytes. Our data show that five of the six VAR2CSA Duffy-binding-like domains are surface exposed and that induction of surface-reactive VAR2CSA-specific IgG depends critically upon antigen conformation. These findings have implications for the development of vaccines against pregnancy-associated Plasmodium falciparum malaria.

AB - We raised specific antisera against recombinant VAR2CSA domains produced in Escherichia coli and in insect cells. All were reactive in enzyme-linked immunosorbent assay, but only insect cell-derived constructs induced immunoglobulin G (IgG) that was reactive with native VAR2CSA on the surface of infected erythrocytes. Our data show that five of the six VAR2CSA Duffy-binding-like domains are surface exposed and that induction of surface-reactive VAR2CSA-specific IgG depends critically upon antigen conformation. These findings have implications for the development of vaccines against pregnancy-associated Plasmodium falciparum malaria.

U2 - 10.1128/IAI.01617-05

DO - 10.1128/IAI.01617-05

M3 - Journal article

C2 - 16790811

VL - 74

SP - 4357

EP - 4360

JO - Infection and Immunity

JF - Infection and Immunity

SN - 0019-9567

IS - 7

ER -

ID: 6746578