Analysis of antibody inhibition of PfEMP1 binding by competition ELISA

Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review

Standard

Analysis of antibody inhibition of PfEMP1 binding by competition ELISA. / Olsen, Rebecca W; Suurbaar, Jennifer; Jensen, Anja Ramstedt.

Malaria Immunology: Targeting the Surface of Infected Erythrocytes. Vol. 2470 Humana Press, 2022. p. 485-491 (Methods in molecular biology (Clifton, N.J.)).

Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review

Harvard

Olsen, RW, Suurbaar, J & Jensen, AR 2022, Analysis of antibody inhibition of PfEMP1 binding by competition ELISA. in Malaria Immunology: Targeting the Surface of Infected Erythrocytes. vol. 2470, Humana Press, Methods in molecular biology (Clifton, N.J.), pp. 485-491. https://doi.org/10.1007/978-1-0716-2189-9_36

APA

Olsen, R. W., Suurbaar, J., & Jensen, A. R. (2022). Analysis of antibody inhibition of PfEMP1 binding by competition ELISA. In Malaria Immunology: Targeting the Surface of Infected Erythrocytes (Vol. 2470, pp. 485-491). Humana Press. Methods in molecular biology (Clifton, N.J.) https://doi.org/10.1007/978-1-0716-2189-9_36

Vancouver

Olsen RW, Suurbaar J, Jensen AR. Analysis of antibody inhibition of PfEMP1 binding by competition ELISA. In Malaria Immunology: Targeting the Surface of Infected Erythrocytes. Vol. 2470. Humana Press. 2022. p. 485-491. (Methods in molecular biology (Clifton, N.J.)). https://doi.org/10.1007/978-1-0716-2189-9_36

Author

Olsen, Rebecca W ; Suurbaar, Jennifer ; Jensen, Anja Ramstedt. / Analysis of antibody inhibition of PfEMP1 binding by competition ELISA. Malaria Immunology: Targeting the Surface of Infected Erythrocytes. Vol. 2470 Humana Press, 2022. pp. 485-491 (Methods in molecular biology (Clifton, N.J.)).

Bibtex

@inbook{54205f31dbee4b0cb319c571ddf20c1c,
title = "Analysis of antibody inhibition of PfEMP1 binding by competition ELISA",
abstract = "Plasmodium falciparum express variant antigens on the surface of infected erythrocytes (IEs). These include proteins of the P. falciparum erythrocyte membrane protein 1 (PfEMP1) family. PfEMP1 proteins mediate binding of IEs to various human endothelial receptors and induce antibodies response during natural infections. In this chapter, we describe a competition ELISA that is an assay for antibodies neutralizing binding of PfEMP1 proteins to their cognate receptor.",
keywords = "Antibodies, Protozoan, Antigens, Protozoan, Enzyme-Linked Immunosorbent Assay, Erythrocytes/metabolism, Humans, Malaria, Falciparum, Plasmodium falciparum/metabolism, Protein Binding, Protozoan Proteins/metabolism",
author = "Olsen, {Rebecca W} and Jennifer Suurbaar and Jensen, {Anja Ramstedt}",
note = "{\textcopyright} 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2022",
doi = "10.1007/978-1-0716-2189-9_36",
language = "English",
volume = "2470",
series = "Methods in molecular biology (Clifton, N.J.)",
publisher = "Humana Press",
pages = "485--491",
booktitle = "Malaria Immunology",
address = "United States",

}

RIS

TY - CHAP

T1 - Analysis of antibody inhibition of PfEMP1 binding by competition ELISA

AU - Olsen, Rebecca W

AU - Suurbaar, Jennifer

AU - Jensen, Anja Ramstedt

N1 - © 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

PY - 2022

Y1 - 2022

N2 - Plasmodium falciparum express variant antigens on the surface of infected erythrocytes (IEs). These include proteins of the P. falciparum erythrocyte membrane protein 1 (PfEMP1) family. PfEMP1 proteins mediate binding of IEs to various human endothelial receptors and induce antibodies response during natural infections. In this chapter, we describe a competition ELISA that is an assay for antibodies neutralizing binding of PfEMP1 proteins to their cognate receptor.

AB - Plasmodium falciparum express variant antigens on the surface of infected erythrocytes (IEs). These include proteins of the P. falciparum erythrocyte membrane protein 1 (PfEMP1) family. PfEMP1 proteins mediate binding of IEs to various human endothelial receptors and induce antibodies response during natural infections. In this chapter, we describe a competition ELISA that is an assay for antibodies neutralizing binding of PfEMP1 proteins to their cognate receptor.

KW - Antibodies, Protozoan

KW - Antigens, Protozoan

KW - Enzyme-Linked Immunosorbent Assay

KW - Erythrocytes/metabolism

KW - Humans

KW - Malaria, Falciparum

KW - Plasmodium falciparum/metabolism

KW - Protein Binding

KW - Protozoan Proteins/metabolism

U2 - 10.1007/978-1-0716-2189-9_36

DO - 10.1007/978-1-0716-2189-9_36

M3 - Book chapter

C2 - 35881368

VL - 2470

T3 - Methods in molecular biology (Clifton, N.J.)

SP - 485

EP - 491

BT - Malaria Immunology

PB - Humana Press

ER -

ID: 320649348