Affinity purification of PfEMP1-specific antibodies from human blood

Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review

Standard

Affinity purification of PfEMP1-specific antibodies from human blood. / Olsen, Rebecca W; Suurbaar, Jennifer; Jensen, Anja Ramstedt.

Malaria Immunology: Targeting the Surface of Infected Erythrocytes. Vol. 2470 Humana Press, 2022. p. 369-379 (Methods in molecular biology (Clifton, N.J.)).

Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review

Harvard

Olsen, RW, Suurbaar, J & Jensen, AR 2022, Affinity purification of PfEMP1-specific antibodies from human blood. in Malaria Immunology: Targeting the Surface of Infected Erythrocytes. vol. 2470, Humana Press, Methods in molecular biology (Clifton, N.J.), pp. 369-379. https://doi.org/10.1007/978-1-0716-2189-9_27

APA

Olsen, R. W., Suurbaar, J., & Jensen, A. R. (2022). Affinity purification of PfEMP1-specific antibodies from human blood. In Malaria Immunology: Targeting the Surface of Infected Erythrocytes (Vol. 2470, pp. 369-379). Humana Press. Methods in molecular biology (Clifton, N.J.) https://doi.org/10.1007/978-1-0716-2189-9_27

Vancouver

Olsen RW, Suurbaar J, Jensen AR. Affinity purification of PfEMP1-specific antibodies from human blood. In Malaria Immunology: Targeting the Surface of Infected Erythrocytes. Vol. 2470. Humana Press. 2022. p. 369-379. (Methods in molecular biology (Clifton, N.J.)). https://doi.org/10.1007/978-1-0716-2189-9_27

Author

Olsen, Rebecca W ; Suurbaar, Jennifer ; Jensen, Anja Ramstedt. / Affinity purification of PfEMP1-specific antibodies from human blood. Malaria Immunology: Targeting the Surface of Infected Erythrocytes. Vol. 2470 Humana Press, 2022. pp. 369-379 (Methods in molecular biology (Clifton, N.J.)).

Bibtex

@inbook{21205f34aae046939405e5e5cd58b9c5,
title = "Affinity purification of PfEMP1-specific antibodies from human blood",
abstract = "Acquired immunity against Plasmodium falciparum infections relies heavily on IgG antibodies specific for PfEMP1 proteins expressed on the surface of infected erythrocytes. Purified human antibodies can be used, for example, to study the interactions between specific PfEMP1 proteins and receptors expressed by human endothelial cells, and to identify which IgG antibodies play a functional role in natural acquired immunity.This chapter describe how to affinity purify PfEMP1-specific human antibodies on an affinity column coupled with PfEMP1 protein. We include ELISA-based methods for identification of human plasma samples reactive against PfEMP1, and for testing of affinity purified IgG antibodies prior to their use in more advanced procedures.",
keywords = "Antibodies, Protozoan, Antigens, Protozoan, Endothelial Cells/metabolism, Erythrocytes/metabolism, Humans, Immunoglobulin G, Malaria, Falciparum, Plasmodium falciparum/metabolism, Protozoan Proteins/metabolism",
author = "Olsen, {Rebecca W} and Jennifer Suurbaar and Jensen, {Anja Ramstedt}",
note = "{\textcopyright} 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2022",
doi = "10.1007/978-1-0716-2189-9_27",
language = "English",
isbn = "978-1-0716-2188-2",
volume = "2470",
series = "Methods in molecular biology (Clifton, N.J.)",
publisher = "Humana Press",
pages = "369--379",
booktitle = "Malaria Immunology",
address = "United States",

}

RIS

TY - CHAP

T1 - Affinity purification of PfEMP1-specific antibodies from human blood

AU - Olsen, Rebecca W

AU - Suurbaar, Jennifer

AU - Jensen, Anja Ramstedt

N1 - © 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

PY - 2022

Y1 - 2022

N2 - Acquired immunity against Plasmodium falciparum infections relies heavily on IgG antibodies specific for PfEMP1 proteins expressed on the surface of infected erythrocytes. Purified human antibodies can be used, for example, to study the interactions between specific PfEMP1 proteins and receptors expressed by human endothelial cells, and to identify which IgG antibodies play a functional role in natural acquired immunity.This chapter describe how to affinity purify PfEMP1-specific human antibodies on an affinity column coupled with PfEMP1 protein. We include ELISA-based methods for identification of human plasma samples reactive against PfEMP1, and for testing of affinity purified IgG antibodies prior to their use in more advanced procedures.

AB - Acquired immunity against Plasmodium falciparum infections relies heavily on IgG antibodies specific for PfEMP1 proteins expressed on the surface of infected erythrocytes. Purified human antibodies can be used, for example, to study the interactions between specific PfEMP1 proteins and receptors expressed by human endothelial cells, and to identify which IgG antibodies play a functional role in natural acquired immunity.This chapter describe how to affinity purify PfEMP1-specific human antibodies on an affinity column coupled with PfEMP1 protein. We include ELISA-based methods for identification of human plasma samples reactive against PfEMP1, and for testing of affinity purified IgG antibodies prior to their use in more advanced procedures.

KW - Antibodies, Protozoan

KW - Antigens, Protozoan

KW - Endothelial Cells/metabolism

KW - Erythrocytes/metabolism

KW - Humans

KW - Immunoglobulin G

KW - Malaria, Falciparum

KW - Plasmodium falciparum/metabolism

KW - Protozoan Proteins/metabolism

U2 - 10.1007/978-1-0716-2189-9_27

DO - 10.1007/978-1-0716-2189-9_27

M3 - Book chapter

C2 - 35881359

SN - 978-1-0716-2188-2

VL - 2470

T3 - Methods in molecular biology (Clifton, N.J.)

SP - 369

EP - 379

BT - Malaria Immunology

PB - Humana Press

ER -

ID: 317099436