3D organoid assay of the impact of infected erythrocyte adhesion on the blood-brain barrier
Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review
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3D organoid assay of the impact of infected erythrocyte adhesion on the blood-brain barrier. / Adams, Yvonne; Jensen, Anja Ramstedt.
Malaria Immunology: Targeting the Surface of Infected Erythrocytes. Vol. 2470 Humana Press, 2022. p. 587-599 (Methods in molecular biology (Clifton, N.J.)).Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review
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TY - CHAP
T1 - 3D organoid assay of the impact of infected erythrocyte adhesion on the blood-brain barrier
AU - Adams, Yvonne
AU - Jensen, Anja Ramstedt
N1 - © 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.
PY - 2022
Y1 - 2022
N2 - Mass sequestration of Plasmodium falciparum parasites in the brain microvasculature can lead to cerebral malaria (CM), characterized by inflammation, vessel occlusion, and brain swelling. To date, only single-cell-type, monolayer assays have been used to investigate the effect of infected erythrocytes (IEs) on the human blood-brain barrier (BBB) and the underlying parenchyma. Here we present a human-derived 3D model of the BBB comprised of endothelial cells, pericytes, and astrocytes in direct contact with each other. The organoids readily self-assemble and can easily be grown in 96-well plates, allowing for high-throughput analysis. These organoids allow for the assessment of parasite adhesion, and analysis of barrier function, and gross morphological changes in response to parasite exposure.
AB - Mass sequestration of Plasmodium falciparum parasites in the brain microvasculature can lead to cerebral malaria (CM), characterized by inflammation, vessel occlusion, and brain swelling. To date, only single-cell-type, monolayer assays have been used to investigate the effect of infected erythrocytes (IEs) on the human blood-brain barrier (BBB) and the underlying parenchyma. Here we present a human-derived 3D model of the BBB comprised of endothelial cells, pericytes, and astrocytes in direct contact with each other. The organoids readily self-assemble and can easily be grown in 96-well plates, allowing for high-throughput analysis. These organoids allow for the assessment of parasite adhesion, and analysis of barrier function, and gross morphological changes in response to parasite exposure.
KW - Blood-Brain Barrier/metabolism
KW - Cell Adhesion/physiology
KW - Endothelial Cells/metabolism
KW - Erythrocytes/metabolism
KW - Humans
KW - Malaria, Falciparum/parasitology
KW - Organoids/metabolism
KW - Plasmodium falciparum/metabolism
KW - Protozoan Proteins/metabolism
U2 - 10.1007/978-1-0716-2189-9_44
DO - 10.1007/978-1-0716-2189-9_44
M3 - Book chapter
C2 - 35881376
VL - 2470
T3 - Methods in molecular biology (Clifton, N.J.)
SP - 587
EP - 599
BT - Malaria Immunology
PB - Humana Press
ER -
ID: 320648836