Ultrasensitive qPCR-Based Detection of Plasmodium falciparum in Pregnant Women Using Dried Blood or Whole Blood Pellet Samples Processed through Different DNA Extraction Methods

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Ultrasensitive qPCR-Based Detection of Plasmodium falciparum in Pregnant Women Using Dried Blood or Whole Blood Pellet Samples Processed through Different DNA Extraction Methods. / Saidi, Queen; Minja, Daniel; Njau, Judith; Hansson, Helle; Kavishe, Reginald; Alifrangis, Michael.

In: The American journal of tropical medicine and hygiene, Vol. 106, No. 3, 2021, p. 846-849.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Saidi, Q, Minja, D, Njau, J, Hansson, H, Kavishe, R & Alifrangis, M 2021, 'Ultrasensitive qPCR-Based Detection of Plasmodium falciparum in Pregnant Women Using Dried Blood or Whole Blood Pellet Samples Processed through Different DNA Extraction Methods', The American journal of tropical medicine and hygiene, vol. 106, no. 3, pp. 846-849. https://doi.org/10.4269/ajtmh.21-0496

APA

Saidi, Q., Minja, D., Njau, J., Hansson, H., Kavishe, R., & Alifrangis, M. (2021). Ultrasensitive qPCR-Based Detection of Plasmodium falciparum in Pregnant Women Using Dried Blood or Whole Blood Pellet Samples Processed through Different DNA Extraction Methods. The American journal of tropical medicine and hygiene, 106(3), 846-849. https://doi.org/10.4269/ajtmh.21-0496

Vancouver

Saidi Q, Minja D, Njau J, Hansson H, Kavishe R, Alifrangis M. Ultrasensitive qPCR-Based Detection of Plasmodium falciparum in Pregnant Women Using Dried Blood or Whole Blood Pellet Samples Processed through Different DNA Extraction Methods. The American journal of tropical medicine and hygiene. 2021;106(3):846-849. https://doi.org/10.4269/ajtmh.21-0496

Author

Saidi, Queen ; Minja, Daniel ; Njau, Judith ; Hansson, Helle ; Kavishe, Reginald ; Alifrangis, Michael. / Ultrasensitive qPCR-Based Detection of Plasmodium falciparum in Pregnant Women Using Dried Blood or Whole Blood Pellet Samples Processed through Different DNA Extraction Methods. In: The American journal of tropical medicine and hygiene. 2021 ; Vol. 106, No. 3. pp. 846-849.

Bibtex

@article{e9d498a17b3a4e92b16be155eb5f74a1,
title = "Ultrasensitive qPCR-Based Detection of Plasmodium falciparum in Pregnant Women Using Dried Blood or Whole Blood Pellet Samples Processed through Different DNA Extraction Methods",
abstract = "Highly sensitive molecular techniques for the detection of low-level Plasmodium falciparum parasitemia are highly useful for various clinical and epidemiological studies. However, differences in how blood samples are preserved, the quantity of blood stored, as well as genomic DNA extraction methods used may compromise the potential usefulness of these methodologies. This study compared diagnostic sensitivity based on microscopy and malaria rapid diagnostic tests (mRDTs), with quantitative polymerase chain reaction (qPCR) P. falciparum positivity of dried blood spots (DBS) or whole blood pellets (WBP) from pregnant women using different DNA extraction protocols (Chelex-saponin or a commercial kit). Samples from 129 pregnant women were analyzed, of which 13 were P. falciparum positive by mRDT and 5 by microscopy. By using extraction kit on WBP and on DBS, qPCR positivity was 27 (20.9%) and 16 (12.4%), respectively, whereas Chelex extraction on DBS only resulted in 4 (3.1%) P. falciparum positive samples. Thus, extraction using commercial kits greatly improve the likelihood of detecting P. falciparum infections.",
author = "Queen Saidi and Daniel Minja and Judith Njau and Helle Hansson and Reginald Kavishe and Michael Alifrangis",
year = "2021",
doi = "10.4269/ajtmh.21-0496",
language = "English",
volume = "106",
pages = "846--849",
journal = "Journal. National Malaria Society",
issn = "0002-9637",
publisher = "American Society of Tropical Medicine and Hygiene",
number = "3",

}

RIS

TY - JOUR

T1 - Ultrasensitive qPCR-Based Detection of Plasmodium falciparum in Pregnant Women Using Dried Blood or Whole Blood Pellet Samples Processed through Different DNA Extraction Methods

AU - Saidi, Queen

AU - Minja, Daniel

AU - Njau, Judith

AU - Hansson, Helle

AU - Kavishe, Reginald

AU - Alifrangis, Michael

PY - 2021

Y1 - 2021

N2 - Highly sensitive molecular techniques for the detection of low-level Plasmodium falciparum parasitemia are highly useful for various clinical and epidemiological studies. However, differences in how blood samples are preserved, the quantity of blood stored, as well as genomic DNA extraction methods used may compromise the potential usefulness of these methodologies. This study compared diagnostic sensitivity based on microscopy and malaria rapid diagnostic tests (mRDTs), with quantitative polymerase chain reaction (qPCR) P. falciparum positivity of dried blood spots (DBS) or whole blood pellets (WBP) from pregnant women using different DNA extraction protocols (Chelex-saponin or a commercial kit). Samples from 129 pregnant women were analyzed, of which 13 were P. falciparum positive by mRDT and 5 by microscopy. By using extraction kit on WBP and on DBS, qPCR positivity was 27 (20.9%) and 16 (12.4%), respectively, whereas Chelex extraction on DBS only resulted in 4 (3.1%) P. falciparum positive samples. Thus, extraction using commercial kits greatly improve the likelihood of detecting P. falciparum infections.

AB - Highly sensitive molecular techniques for the detection of low-level Plasmodium falciparum parasitemia are highly useful for various clinical and epidemiological studies. However, differences in how blood samples are preserved, the quantity of blood stored, as well as genomic DNA extraction methods used may compromise the potential usefulness of these methodologies. This study compared diagnostic sensitivity based on microscopy and malaria rapid diagnostic tests (mRDTs), with quantitative polymerase chain reaction (qPCR) P. falciparum positivity of dried blood spots (DBS) or whole blood pellets (WBP) from pregnant women using different DNA extraction protocols (Chelex-saponin or a commercial kit). Samples from 129 pregnant women were analyzed, of which 13 were P. falciparum positive by mRDT and 5 by microscopy. By using extraction kit on WBP and on DBS, qPCR positivity was 27 (20.9%) and 16 (12.4%), respectively, whereas Chelex extraction on DBS only resulted in 4 (3.1%) P. falciparum positive samples. Thus, extraction using commercial kits greatly improve the likelihood of detecting P. falciparum infections.

U2 - 10.4269/ajtmh.21-0496

DO - 10.4269/ajtmh.21-0496

M3 - Journal article

C2 - 34872057

AN - SCOPUS:85126152398

VL - 106

SP - 846

EP - 849

JO - Journal. National Malaria Society

JF - Journal. National Malaria Society

SN - 0002-9637

IS - 3

ER -

ID: 317672814