Preservation and extraction of malaria parasite DNA from dried blood spots

Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review

Standard

Preservation and extraction of malaria parasite DNA from dried blood spots. / Hansson, Helle; Saidi, Queen; Alifrangis, Michael.

Malaria Immunology: Targeting the Surface of Infected Erythrocytes. Vol. 2470 Human Kinetics, 2022. p. 27-36 (Methods in molecular biology (Clifton, N.J.)).

Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review

Harvard

Hansson, H, Saidi, Q & Alifrangis, M 2022, Preservation and extraction of malaria parasite DNA from dried blood spots. in Malaria Immunology: Targeting the Surface of Infected Erythrocytes. vol. 2470, Human Kinetics, Methods in molecular biology (Clifton, N.J.), pp. 27-36. https://doi.org/10.1007/978-1-0716-2189-9_4

APA

Hansson, H., Saidi, Q., & Alifrangis, M. (2022). Preservation and extraction of malaria parasite DNA from dried blood spots. In Malaria Immunology: Targeting the Surface of Infected Erythrocytes (Vol. 2470, pp. 27-36). Human Kinetics. Methods in molecular biology (Clifton, N.J.) https://doi.org/10.1007/978-1-0716-2189-9_4

Vancouver

Hansson H, Saidi Q, Alifrangis M. Preservation and extraction of malaria parasite DNA from dried blood spots. In Malaria Immunology: Targeting the Surface of Infected Erythrocytes. Vol. 2470. Human Kinetics. 2022. p. 27-36. (Methods in molecular biology (Clifton, N.J.)). https://doi.org/10.1007/978-1-0716-2189-9_4

Author

Hansson, Helle ; Saidi, Queen ; Alifrangis, Michael. / Preservation and extraction of malaria parasite DNA from dried blood spots. Malaria Immunology: Targeting the Surface of Infected Erythrocytes. Vol. 2470 Human Kinetics, 2022. pp. 27-36 (Methods in molecular biology (Clifton, N.J.)).

Bibtex

@inbook{8e4332238e224f9c8ef90e6dadd1a4b9,
title = "Preservation and extraction of malaria parasite DNA from dried blood spots",
abstract = "Molecular studies related to diagnosis and research rely on collection of blood samples and extraction of high-quality DNA. In Africa, where the populations carried 94% of the total burden of cases and deaths due to malaria in 2019, collection of samples is often challenged by remote study areas and lack of a cold chain to transport and store samples. Collection of blood on filter paper is a technique that is less invasive and has simpler requirements regarding training of staff, storage, and transport of samples than collection of venous blood samples. Dried blood spots (DBS) are therefore commonly used in many research projects. However, DNA quality can be affected by duration and conditions of storage. The quality of the DNA for molecular analyses also depends on a DNA extraction methodology that provides high-quality DNA with high purity and yield. Several protocols for DNA extraction have been described, and many comparative studies have analyzed and optimized the different methodologies to find an alternative to the more costly commercial extraction kits. This chapter describes recommendations for storage and preservation of DBS, and a Chelex-based protocol for extraction of DNA from DBS.",
keywords = "Animals, DNA, Protozoan/genetics, Humans, Malaria, Falciparum/parasitology, Parasites/genetics, Plasmodium falciparum/genetics, Polymerase Chain Reaction/methods, Specimen Handling/methods",
author = "Helle Hansson and Queen Saidi and Michael Alifrangis",
note = "{\textcopyright} 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2022",
doi = "10.1007/978-1-0716-2189-9_4",
language = "English",
volume = "2470",
series = "Methods in molecular biology (Clifton, N.J.)",
publisher = "Human Kinetics",
pages = "27--36",
booktitle = "Malaria Immunology",
address = "United States",

}

RIS

TY - CHAP

T1 - Preservation and extraction of malaria parasite DNA from dried blood spots

AU - Hansson, Helle

AU - Saidi, Queen

AU - Alifrangis, Michael

N1 - © 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

PY - 2022

Y1 - 2022

N2 - Molecular studies related to diagnosis and research rely on collection of blood samples and extraction of high-quality DNA. In Africa, where the populations carried 94% of the total burden of cases and deaths due to malaria in 2019, collection of samples is often challenged by remote study areas and lack of a cold chain to transport and store samples. Collection of blood on filter paper is a technique that is less invasive and has simpler requirements regarding training of staff, storage, and transport of samples than collection of venous blood samples. Dried blood spots (DBS) are therefore commonly used in many research projects. However, DNA quality can be affected by duration and conditions of storage. The quality of the DNA for molecular analyses also depends on a DNA extraction methodology that provides high-quality DNA with high purity and yield. Several protocols for DNA extraction have been described, and many comparative studies have analyzed and optimized the different methodologies to find an alternative to the more costly commercial extraction kits. This chapter describes recommendations for storage and preservation of DBS, and a Chelex-based protocol for extraction of DNA from DBS.

AB - Molecular studies related to diagnosis and research rely on collection of blood samples and extraction of high-quality DNA. In Africa, where the populations carried 94% of the total burden of cases and deaths due to malaria in 2019, collection of samples is often challenged by remote study areas and lack of a cold chain to transport and store samples. Collection of blood on filter paper is a technique that is less invasive and has simpler requirements regarding training of staff, storage, and transport of samples than collection of venous blood samples. Dried blood spots (DBS) are therefore commonly used in many research projects. However, DNA quality can be affected by duration and conditions of storage. The quality of the DNA for molecular analyses also depends on a DNA extraction methodology that provides high-quality DNA with high purity and yield. Several protocols for DNA extraction have been described, and many comparative studies have analyzed and optimized the different methodologies to find an alternative to the more costly commercial extraction kits. This chapter describes recommendations for storage and preservation of DBS, and a Chelex-based protocol for extraction of DNA from DBS.

KW - Animals

KW - DNA, Protozoan/genetics

KW - Humans

KW - Malaria, Falciparum/parasitology

KW - Parasites/genetics

KW - Plasmodium falciparum/genetics

KW - Polymerase Chain Reaction/methods

KW - Specimen Handling/methods

U2 - 10.1007/978-1-0716-2189-9_4

DO - 10.1007/978-1-0716-2189-9_4

M3 - Book chapter

C2 - 35881336

VL - 2470

T3 - Methods in molecular biology (Clifton, N.J.)

SP - 27

EP - 36

BT - Malaria Immunology

PB - Human Kinetics

ER -

ID: 320648965