Unravelling the Phylogeny of a Common Intestinal Protist: Intrageneric Diversity of Endolimax
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Unravelling the Phylogeny of a Common Intestinal Protist : Intrageneric Diversity of Endolimax. / Hocke, Emma Filtenborg; Jamy, Mahwash; Burki, Fabien; Clark, C Graham; Stensvold, Christen Rune.
In: Protist, Vol. 173, No. 5, 125908, 2022.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Unravelling the Phylogeny of a Common Intestinal Protist
T2 - Intrageneric Diversity of Endolimax
AU - Hocke, Emma Filtenborg
AU - Jamy, Mahwash
AU - Burki, Fabien
AU - Clark, C Graham
AU - Stensvold, Christen Rune
N1 - Crown Copyright © 2022. Published by Elsevier GmbH. All rights reserved.
PY - 2022
Y1 - 2022
N2 - Endolimax nana is a common endobiont of the human intestine, but members of the genus have also been reported in non-human hosts and in non-intestinal organs. Limited information is available regarding the genetic diversity of Endolimax, which is necessary to delineate species, host specificity and potential differences in clinical impact on the host. Here, we used cloning of PCR products followed by Sanger sequencing and next-generation PacBio Sequencing to obtain Endolimax-related nuclear ribosomal gene sequences and undertook a phylogenetic analysis to gain additional insight into the taxonomy of Endolimax and related organisms. The new sequences confirmed that E. nana forms a discrete clade within the Archamoebae and is related to Endolimax piscium and Iodamoeba. However, we identified substantial sequence divergence within E. nana and evidence for two distinct clades, which we propose to name E. nana ribosomal lineage 1 and E. nana ribosomal lineage 2. Both of the sequencing approaches applied in the study helped us to improve our understanding of genetic diversity across Endolimax, and it is likely that wider application of next-generation sequencing technologies will facilitate the generation of Endolimax-related DNA sequence data and help complete our understanding of its phylogenetic position and intrageneric diversity.
AB - Endolimax nana is a common endobiont of the human intestine, but members of the genus have also been reported in non-human hosts and in non-intestinal organs. Limited information is available regarding the genetic diversity of Endolimax, which is necessary to delineate species, host specificity and potential differences in clinical impact on the host. Here, we used cloning of PCR products followed by Sanger sequencing and next-generation PacBio Sequencing to obtain Endolimax-related nuclear ribosomal gene sequences and undertook a phylogenetic analysis to gain additional insight into the taxonomy of Endolimax and related organisms. The new sequences confirmed that E. nana forms a discrete clade within the Archamoebae and is related to Endolimax piscium and Iodamoeba. However, we identified substantial sequence divergence within E. nana and evidence for two distinct clades, which we propose to name E. nana ribosomal lineage 1 and E. nana ribosomal lineage 2. Both of the sequencing approaches applied in the study helped us to improve our understanding of genetic diversity across Endolimax, and it is likely that wider application of next-generation sequencing technologies will facilitate the generation of Endolimax-related DNA sequence data and help complete our understanding of its phylogenetic position and intrageneric diversity.
U2 - 10.1016/j.protis.2022.125908
DO - 10.1016/j.protis.2022.125908
M3 - Journal article
C2 - 36152390
VL - 173
JO - Protist
JF - Protist
SN - 1434-4610
IS - 5
M1 - 125908
ER -
ID: 321836453