Performance of a histidine rich protein-2 based (First Response) and a p-lactate dehydrogenase-based (Optimal) rapid diagnostic test for diagnosis of malaria in patients with pediatric sickle cell disease

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Performance of a histidine rich protein-2 based (First Response) and a p-lactate dehydrogenase-based (Optimal) rapid diagnostic test for diagnosis of malaria in patients with pediatric sickle cell disease. / Adjei, George O.; Sulley, Abdul M.; Goka, Bamenla Q.; Enweronu-Laryea, Christabel; Renner, Lorna; Alifrangis, Michael; Kurtzhals, Jorgen A.L.

In: Clinical infectious diseases : an official publication of the Infectious Diseases Society of America, Vol. 75, No. 3, 2022, p. 435-441.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Adjei, GO, Sulley, AM, Goka, BQ, Enweronu-Laryea, C, Renner, L, Alifrangis, M & Kurtzhals, JAL 2022, 'Performance of a histidine rich protein-2 based (First Response) and a p-lactate dehydrogenase-based (Optimal) rapid diagnostic test for diagnosis of malaria in patients with pediatric sickle cell disease', Clinical infectious diseases : an official publication of the Infectious Diseases Society of America, vol. 75, no. 3, pp. 435-441. https://doi.org/10.1093/cid/ciab977

APA

Adjei, G. O., Sulley, A. M., Goka, B. Q., Enweronu-Laryea, C., Renner, L., Alifrangis, M., & Kurtzhals, J. A. L. (2022). Performance of a histidine rich protein-2 based (First Response) and a p-lactate dehydrogenase-based (Optimal) rapid diagnostic test for diagnosis of malaria in patients with pediatric sickle cell disease. Clinical infectious diseases : an official publication of the Infectious Diseases Society of America, 75(3), 435-441. https://doi.org/10.1093/cid/ciab977

Vancouver

Adjei GO, Sulley AM, Goka BQ, Enweronu-Laryea C, Renner L, Alifrangis M et al. Performance of a histidine rich protein-2 based (First Response) and a p-lactate dehydrogenase-based (Optimal) rapid diagnostic test for diagnosis of malaria in patients with pediatric sickle cell disease. Clinical infectious diseases : an official publication of the Infectious Diseases Society of America. 2022;75(3):435-441. https://doi.org/10.1093/cid/ciab977

Author

Adjei, George O. ; Sulley, Abdul M. ; Goka, Bamenla Q. ; Enweronu-Laryea, Christabel ; Renner, Lorna ; Alifrangis, Michael ; Kurtzhals, Jorgen A.L. / Performance of a histidine rich protein-2 based (First Response) and a p-lactate dehydrogenase-based (Optimal) rapid diagnostic test for diagnosis of malaria in patients with pediatric sickle cell disease. In: Clinical infectious diseases : an official publication of the Infectious Diseases Society of America. 2022 ; Vol. 75, No. 3. pp. 435-441.

Bibtex

@article{2d0bc20a5e9f46b1a94f52d938e627c1,
title = "Performance of a histidine rich protein-2 based (First Response) and a p-lactate dehydrogenase-based (Optimal) rapid diagnostic test for diagnosis of malaria in patients with pediatric sickle cell disease",
abstract = "BACKGROUND: Rapid diagnostic tests (RDTs) have been extensively evaluated and play an important role in malaria diagnosis. However, the accuracy of RDTs for malaria diagnosis in patients with sickle cell disease (SCD) is unknown. METHODS: We compared the performance of a histidine rich protein 2 (HRP-2)-based RDT (First Response) and a lactate dehydrogenase (LDH)-based RDT (Optimal) with routine microscopy as reference standard in 445 children with SCD and an acute febrile illness in Accra, Ghana. RESULTS: The overall sensitivity, specificity, and positive and negative predictive values of the HRP-2-based RDTs were 100%, 95.7%, 73.8%, and 100%, respectively. Comparable values for the LDH-based RDTs were 91.7%, 99.5%, 95.7%, and 99.0%, respectively. A total of 423 results were true in both tests, 1 result was false in both tests, 16 results were false in the HRP-2 test only, and 5 were false in the LDH test only (McNemar test, P = .03). At follow-up, 73.7% (28/38), 52.6% (20/38), 48.6% (17/35), and 13.2% (5/38) of study participants were HRP-2 positive on days 14, 28, 35, and 42, respectively, compared with 0%, 2.6% (1/38), 2.9% (1/35), and 2.6% (1/38) for LDH. CONCLUSION: The HRP2-based RDT fulfilled World Health Organization criteria for malaria diagnosis in patients with SCD and may provide diagnostic evidence for treatment to begin in cases in which treatment would otherwise have begun presumptively based on symptoms, whereas LDH-based RDTs may be more suitable as a confirmatory test in low-parasitemic subgroups, such as patients with SCD.",
keywords = "HRP-2, malaria, p-LDH, rapid diagnostic test, sickle cell disease",
author = "Adjei, {George O.} and Sulley, {Abdul M.} and Goka, {Bamenla Q.} and Christabel Enweronu-Laryea and Lorna Renner and Michael Alifrangis and Kurtzhals, {Jorgen A.L.}",
note = "Publisher Copyright: {\textcopyright} The Author(s) 2021. Published by Oxford University Press for the Infectious Diseases Society of America. All rights reserved. For permissions, e-mail: journals.permissions@oup.com.",
year = "2022",
doi = "10.1093/cid/ciab977",
language = "English",
volume = "75",
pages = "435--441",
journal = "Clinical Infectious Diseases",
issn = "1058-4838",
publisher = "Oxford University Press",
number = "3",

}

RIS

TY - JOUR

T1 - Performance of a histidine rich protein-2 based (First Response) and a p-lactate dehydrogenase-based (Optimal) rapid diagnostic test for diagnosis of malaria in patients with pediatric sickle cell disease

AU - Adjei, George O.

AU - Sulley, Abdul M.

AU - Goka, Bamenla Q.

AU - Enweronu-Laryea, Christabel

AU - Renner, Lorna

AU - Alifrangis, Michael

AU - Kurtzhals, Jorgen A.L.

N1 - Publisher Copyright: © The Author(s) 2021. Published by Oxford University Press for the Infectious Diseases Society of America. All rights reserved. For permissions, e-mail: journals.permissions@oup.com.

PY - 2022

Y1 - 2022

N2 - BACKGROUND: Rapid diagnostic tests (RDTs) have been extensively evaluated and play an important role in malaria diagnosis. However, the accuracy of RDTs for malaria diagnosis in patients with sickle cell disease (SCD) is unknown. METHODS: We compared the performance of a histidine rich protein 2 (HRP-2)-based RDT (First Response) and a lactate dehydrogenase (LDH)-based RDT (Optimal) with routine microscopy as reference standard in 445 children with SCD and an acute febrile illness in Accra, Ghana. RESULTS: The overall sensitivity, specificity, and positive and negative predictive values of the HRP-2-based RDTs were 100%, 95.7%, 73.8%, and 100%, respectively. Comparable values for the LDH-based RDTs were 91.7%, 99.5%, 95.7%, and 99.0%, respectively. A total of 423 results were true in both tests, 1 result was false in both tests, 16 results were false in the HRP-2 test only, and 5 were false in the LDH test only (McNemar test, P = .03). At follow-up, 73.7% (28/38), 52.6% (20/38), 48.6% (17/35), and 13.2% (5/38) of study participants were HRP-2 positive on days 14, 28, 35, and 42, respectively, compared with 0%, 2.6% (1/38), 2.9% (1/35), and 2.6% (1/38) for LDH. CONCLUSION: The HRP2-based RDT fulfilled World Health Organization criteria for malaria diagnosis in patients with SCD and may provide diagnostic evidence for treatment to begin in cases in which treatment would otherwise have begun presumptively based on symptoms, whereas LDH-based RDTs may be more suitable as a confirmatory test in low-parasitemic subgroups, such as patients with SCD.

AB - BACKGROUND: Rapid diagnostic tests (RDTs) have been extensively evaluated and play an important role in malaria diagnosis. However, the accuracy of RDTs for malaria diagnosis in patients with sickle cell disease (SCD) is unknown. METHODS: We compared the performance of a histidine rich protein 2 (HRP-2)-based RDT (First Response) and a lactate dehydrogenase (LDH)-based RDT (Optimal) with routine microscopy as reference standard in 445 children with SCD and an acute febrile illness in Accra, Ghana. RESULTS: The overall sensitivity, specificity, and positive and negative predictive values of the HRP-2-based RDTs were 100%, 95.7%, 73.8%, and 100%, respectively. Comparable values for the LDH-based RDTs were 91.7%, 99.5%, 95.7%, and 99.0%, respectively. A total of 423 results were true in both tests, 1 result was false in both tests, 16 results were false in the HRP-2 test only, and 5 were false in the LDH test only (McNemar test, P = .03). At follow-up, 73.7% (28/38), 52.6% (20/38), 48.6% (17/35), and 13.2% (5/38) of study participants were HRP-2 positive on days 14, 28, 35, and 42, respectively, compared with 0%, 2.6% (1/38), 2.9% (1/35), and 2.6% (1/38) for LDH. CONCLUSION: The HRP2-based RDT fulfilled World Health Organization criteria for malaria diagnosis in patients with SCD and may provide diagnostic evidence for treatment to begin in cases in which treatment would otherwise have begun presumptively based on symptoms, whereas LDH-based RDTs may be more suitable as a confirmatory test in low-parasitemic subgroups, such as patients with SCD.

KW - HRP-2

KW - malaria

KW - p-LDH

KW - rapid diagnostic test

KW - sickle cell disease

U2 - 10.1093/cid/ciab977

DO - 10.1093/cid/ciab977

M3 - Journal article

C2 - 34849647

AN - SCOPUS:85134544046

VL - 75

SP - 435

EP - 441

JO - Clinical Infectious Diseases

JF - Clinical Infectious Diseases

SN - 1058-4838

IS - 3

ER -

ID: 319750860