Measuring rosetting inhibition in Plasmodium falciparum parasites using a flow cytometry-based assay

Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review

Standard

Measuring rosetting inhibition in Plasmodium falciparum parasites using a flow cytometry-based assay. / Quintana, Maria Del Pilar; Ch'ng, Jun-Hong.

Malaria Immunology: Targeting the Surface of Infected Erythrocytes. Vol. 2470 2022. p. 493-503 (Methods in molecular biology (Clifton, N.J.)).

Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review

Harvard

Quintana, MDP & Ch'ng, J-H 2022, Measuring rosetting inhibition in Plasmodium falciparum parasites using a flow cytometry-based assay. in Malaria Immunology: Targeting the Surface of Infected Erythrocytes. vol. 2470, Methods in molecular biology (Clifton, N.J.), pp. 493-503. https://doi.org/10.1007/978-1-0716-2189-9_37

APA

Quintana, M. D. P., & Ch'ng, J-H. (2022). Measuring rosetting inhibition in Plasmodium falciparum parasites using a flow cytometry-based assay. In Malaria Immunology: Targeting the Surface of Infected Erythrocytes (Vol. 2470, pp. 493-503). Methods in molecular biology (Clifton, N.J.) https://doi.org/10.1007/978-1-0716-2189-9_37

Vancouver

Quintana MDP, Ch'ng J-H. Measuring rosetting inhibition in Plasmodium falciparum parasites using a flow cytometry-based assay. In Malaria Immunology: Targeting the Surface of Infected Erythrocytes. Vol. 2470. 2022. p. 493-503. (Methods in molecular biology (Clifton, N.J.)). https://doi.org/10.1007/978-1-0716-2189-9_37

Author

Quintana, Maria Del Pilar ; Ch'ng, Jun-Hong. / Measuring rosetting inhibition in Plasmodium falciparum parasites using a flow cytometry-based assay. Malaria Immunology: Targeting the Surface of Infected Erythrocytes. Vol. 2470 2022. pp. 493-503 (Methods in molecular biology (Clifton, N.J.)).

Bibtex

@inbook{74309e81b45141aa834d6ab3050811d5,
title = "Measuring rosetting inhibition in Plasmodium falciparum parasites using a flow cytometry-based assay",
abstract = "Rosetting is the ability of Plasmodium falciparum-infected erythrocytes (IEs) to bind to host receptors on the surface of uninfected erythrocytes (uE) leading to the formation of a cluster of cells with a central IE surrounded by uE. It is a hallmark event during the pathogenesis of P. falciparum malaria, the most severe species causing malaria, which affects mostly young children in Africa. There are no current treatments effectively targeting and disrupting parasite rosette formation. Here, we detail a high-throughput, flow cytometry based assay that allows testing and identification of potential rosetting-inhibitory compounds that could be used in combination with anti-plasmodial drugs to reduce malaria morbidity and mortality.",
keywords = "Animals, Child, Child, Preschool, Erythrocytes/metabolism, Flow Cytometry, Humans, Malaria, Falciparum/parasitology, Parasites, Plasmodium falciparum/physiology, Rosette Formation",
author = "Quintana, {Maria Del Pilar} and Jun-Hong Ch'ng",
note = "{\textcopyright} 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2022",
doi = "10.1007/978-1-0716-2189-9_37",
language = "English",
isbn = "978-1-0716-2188-2",
volume = "2470",
series = "Methods in molecular biology (Clifton, N.J.)",
publisher = "Humana Press",
pages = "493--503",
booktitle = "Malaria Immunology",

}

RIS

TY - CHAP

T1 - Measuring rosetting inhibition in Plasmodium falciparum parasites using a flow cytometry-based assay

AU - Quintana, Maria Del Pilar

AU - Ch'ng, Jun-Hong

N1 - © 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

PY - 2022

Y1 - 2022

N2 - Rosetting is the ability of Plasmodium falciparum-infected erythrocytes (IEs) to bind to host receptors on the surface of uninfected erythrocytes (uE) leading to the formation of a cluster of cells with a central IE surrounded by uE. It is a hallmark event during the pathogenesis of P. falciparum malaria, the most severe species causing malaria, which affects mostly young children in Africa. There are no current treatments effectively targeting and disrupting parasite rosette formation. Here, we detail a high-throughput, flow cytometry based assay that allows testing and identification of potential rosetting-inhibitory compounds that could be used in combination with anti-plasmodial drugs to reduce malaria morbidity and mortality.

AB - Rosetting is the ability of Plasmodium falciparum-infected erythrocytes (IEs) to bind to host receptors on the surface of uninfected erythrocytes (uE) leading to the formation of a cluster of cells with a central IE surrounded by uE. It is a hallmark event during the pathogenesis of P. falciparum malaria, the most severe species causing malaria, which affects mostly young children in Africa. There are no current treatments effectively targeting and disrupting parasite rosette formation. Here, we detail a high-throughput, flow cytometry based assay that allows testing and identification of potential rosetting-inhibitory compounds that could be used in combination with anti-plasmodial drugs to reduce malaria morbidity and mortality.

KW - Animals

KW - Child

KW - Child, Preschool

KW - Erythrocytes/metabolism

KW - Flow Cytometry

KW - Humans

KW - Malaria, Falciparum/parasitology

KW - Parasites

KW - Plasmodium falciparum/physiology

KW - Rosette Formation

U2 - 10.1007/978-1-0716-2189-9_37

DO - 10.1007/978-1-0716-2189-9_37

M3 - Book chapter

C2 - 35881369

SN - 978-1-0716-2188-2

VL - 2470

T3 - Methods in molecular biology (Clifton, N.J.)

SP - 493

EP - 503

BT - Malaria Immunology

ER -

ID: 317099505