Identification of the PfK13 mutations R561H and P441L in the Democratic Republic of Congo

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Identification of the PfK13 mutations R561H and P441L in the Democratic Republic of Congo. / Mesia Kahunu, Gauthier; Wellmann Thomsen, Sarah; Wellmann Thomsen, Louise; Muhindo Mavoko, Hypolite; Mitashi Mulopo, Patrick; Filtenborg Hocke, Emma; Mandoko Nkoli, Papy; Baraka, Vito; Minja, Daniel T.R.; Mousa, Andria; Roper, Cally; Mbongi Moke, Destin; Mumba Ngoyi, Dieudonné; Mukomena Sompwe, Eric; Muyembe Tanfum, Jean Jacques; Hansson, Helle; Alifrangis, Michael.

In: International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases, Vol. 139, 2024, p. 41-49.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Mesia Kahunu, G, Wellmann Thomsen, S, Wellmann Thomsen, L, Muhindo Mavoko, H, Mitashi Mulopo, P, Filtenborg Hocke, E, Mandoko Nkoli, P, Baraka, V, Minja, DTR, Mousa, A, Roper, C, Mbongi Moke, D, Mumba Ngoyi, D, Mukomena Sompwe, E, Muyembe Tanfum, JJ, Hansson, H & Alifrangis, M 2024, 'Identification of the PfK13 mutations R561H and P441L in the Democratic Republic of Congo', International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases, vol. 139, pp. 41-49. https://doi.org/10.1016/j.ijid.2023.11.026

APA

Mesia Kahunu, G., Wellmann Thomsen, S., Wellmann Thomsen, L., Muhindo Mavoko, H., Mitashi Mulopo, P., Filtenborg Hocke, E., Mandoko Nkoli, P., Baraka, V., Minja, D. T. R., Mousa, A., Roper, C., Mbongi Moke, D., Mumba Ngoyi, D., Mukomena Sompwe, E., Muyembe Tanfum, J. J., Hansson, H., & Alifrangis, M. (2024). Identification of the PfK13 mutations R561H and P441L in the Democratic Republic of Congo. International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases, 139, 41-49. https://doi.org/10.1016/j.ijid.2023.11.026

Vancouver

Mesia Kahunu G, Wellmann Thomsen S, Wellmann Thomsen L, Muhindo Mavoko H, Mitashi Mulopo P, Filtenborg Hocke E et al. Identification of the PfK13 mutations R561H and P441L in the Democratic Republic of Congo. International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases. 2024;139:41-49. https://doi.org/10.1016/j.ijid.2023.11.026

Author

Mesia Kahunu, Gauthier ; Wellmann Thomsen, Sarah ; Wellmann Thomsen, Louise ; Muhindo Mavoko, Hypolite ; Mitashi Mulopo, Patrick ; Filtenborg Hocke, Emma ; Mandoko Nkoli, Papy ; Baraka, Vito ; Minja, Daniel T.R. ; Mousa, Andria ; Roper, Cally ; Mbongi Moke, Destin ; Mumba Ngoyi, Dieudonné ; Mukomena Sompwe, Eric ; Muyembe Tanfum, Jean Jacques ; Hansson, Helle ; Alifrangis, Michael. / Identification of the PfK13 mutations R561H and P441L in the Democratic Republic of Congo. In: International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases. 2024 ; Vol. 139. pp. 41-49.

Bibtex

@article{d3ed836a59314b9b9eeb173ec1129a01,
title = "Identification of the PfK13 mutations R561H and P441L in the Democratic Republic of Congo",
abstract = "OBJECTIVES: Partial artemisinin resistance, mediated by Plasmodium falciparum K13 (PfK13) mutations, has been confirmed in certain areas of East Africa that are historically associated with high-level antimalarial resistance. The Democratic Republic of Congo (DRC) borders these areas in the East. This study aimed to determine the prevalence of resistance markers in six National Malaria Control Program surveillance sites; Boende, Kabondo, Kapolowe, Kimpese, Mikalayi, and Rutshuru. METHODS: The single nucleotide polymorphisms (SNPs) in P. falciparum genes PfK13, Pfdhfr, Pfdhps, Pfmdr1, and Pfcrt were assessed using targeted next-generation sequencing of isolates collected at enrollment in therapeutic efficacy studies. RESULTS: PfK13 SNPs were detected in two samples: in Kabondo (R561H) and in Rutshuru (P441L), both areas near Uganda and Rwanda. The Pfdhps ISGEGA haplotype, associated with reduced sulfadoxine-pyrimethamine chemoprevention efficacy, ranged from 0.8% in Mikalayi (central DRC) to 42.2% in Rutshuru (East DRC). CONCLUSIONS: R561H and P441L observed in eastern DRC are a concern, as they are associated with delayed artemisinin-based combination therapies-clearance and candidate marker of resistance, respectively. This is consistent with previous observations of shared drug resistance profiles in parasites of that region with bordering areas of Rwanda and Uganda. The likely circulation of parasites has important implications for the ongoing surveillance of partial artemisinin-resistant P. falciparum and for future efforts to mitigate its dispersal.",
keywords = "Antimalarial drugs, Artemisinin, PfK13, Democratic Republic of Congo, Drug resistance",
author = "{Mesia Kahunu}, Gauthier and {Wellmann Thomsen}, Sarah and {Wellmann Thomsen}, Louise and {Muhindo Mavoko}, Hypolite and {Mitashi Mulopo}, Patrick and {Filtenborg Hocke}, Emma and {Mandoko Nkoli}, Papy and Vito Baraka and Minja, {Daniel T.R.} and Andria Mousa and Cally Roper and {Mbongi Moke}, Destin and {Mumba Ngoyi}, Dieudonn{\'e} and {Mukomena Sompwe}, Eric and {Muyembe Tanfum}, {Jean Jacques} and Helle Hansson and Michael Alifrangis",
note = "Publisher Copyright: Copyright {\textcopyright} 2023 The Author(s). Published by Elsevier Ltd.. All rights reserved.",
year = "2024",
doi = "10.1016/j.ijid.2023.11.026",
language = "English",
volume = "139",
pages = "41--49",
journal = "International Journal of Infectious Diseases",
issn = "1201-9712",
publisher = "Elsevier",

}

RIS

TY - JOUR

T1 - Identification of the PfK13 mutations R561H and P441L in the Democratic Republic of Congo

AU - Mesia Kahunu, Gauthier

AU - Wellmann Thomsen, Sarah

AU - Wellmann Thomsen, Louise

AU - Muhindo Mavoko, Hypolite

AU - Mitashi Mulopo, Patrick

AU - Filtenborg Hocke, Emma

AU - Mandoko Nkoli, Papy

AU - Baraka, Vito

AU - Minja, Daniel T.R.

AU - Mousa, Andria

AU - Roper, Cally

AU - Mbongi Moke, Destin

AU - Mumba Ngoyi, Dieudonné

AU - Mukomena Sompwe, Eric

AU - Muyembe Tanfum, Jean Jacques

AU - Hansson, Helle

AU - Alifrangis, Michael

N1 - Publisher Copyright: Copyright © 2023 The Author(s). Published by Elsevier Ltd.. All rights reserved.

PY - 2024

Y1 - 2024

N2 - OBJECTIVES: Partial artemisinin resistance, mediated by Plasmodium falciparum K13 (PfK13) mutations, has been confirmed in certain areas of East Africa that are historically associated with high-level antimalarial resistance. The Democratic Republic of Congo (DRC) borders these areas in the East. This study aimed to determine the prevalence of resistance markers in six National Malaria Control Program surveillance sites; Boende, Kabondo, Kapolowe, Kimpese, Mikalayi, and Rutshuru. METHODS: The single nucleotide polymorphisms (SNPs) in P. falciparum genes PfK13, Pfdhfr, Pfdhps, Pfmdr1, and Pfcrt were assessed using targeted next-generation sequencing of isolates collected at enrollment in therapeutic efficacy studies. RESULTS: PfK13 SNPs were detected in two samples: in Kabondo (R561H) and in Rutshuru (P441L), both areas near Uganda and Rwanda. The Pfdhps ISGEGA haplotype, associated with reduced sulfadoxine-pyrimethamine chemoprevention efficacy, ranged from 0.8% in Mikalayi (central DRC) to 42.2% in Rutshuru (East DRC). CONCLUSIONS: R561H and P441L observed in eastern DRC are a concern, as they are associated with delayed artemisinin-based combination therapies-clearance and candidate marker of resistance, respectively. This is consistent with previous observations of shared drug resistance profiles in parasites of that region with bordering areas of Rwanda and Uganda. The likely circulation of parasites has important implications for the ongoing surveillance of partial artemisinin-resistant P. falciparum and for future efforts to mitigate its dispersal.

AB - OBJECTIVES: Partial artemisinin resistance, mediated by Plasmodium falciparum K13 (PfK13) mutations, has been confirmed in certain areas of East Africa that are historically associated with high-level antimalarial resistance. The Democratic Republic of Congo (DRC) borders these areas in the East. This study aimed to determine the prevalence of resistance markers in six National Malaria Control Program surveillance sites; Boende, Kabondo, Kapolowe, Kimpese, Mikalayi, and Rutshuru. METHODS: The single nucleotide polymorphisms (SNPs) in P. falciparum genes PfK13, Pfdhfr, Pfdhps, Pfmdr1, and Pfcrt were assessed using targeted next-generation sequencing of isolates collected at enrollment in therapeutic efficacy studies. RESULTS: PfK13 SNPs were detected in two samples: in Kabondo (R561H) and in Rutshuru (P441L), both areas near Uganda and Rwanda. The Pfdhps ISGEGA haplotype, associated with reduced sulfadoxine-pyrimethamine chemoprevention efficacy, ranged from 0.8% in Mikalayi (central DRC) to 42.2% in Rutshuru (East DRC). CONCLUSIONS: R561H and P441L observed in eastern DRC are a concern, as they are associated with delayed artemisinin-based combination therapies-clearance and candidate marker of resistance, respectively. This is consistent with previous observations of shared drug resistance profiles in parasites of that region with bordering areas of Rwanda and Uganda. The likely circulation of parasites has important implications for the ongoing surveillance of partial artemisinin-resistant P. falciparum and for future efforts to mitigate its dispersal.

KW - Antimalarial drugs

KW - Artemisinin, PfK13

KW - Democratic Republic of Congo

KW - Drug resistance

U2 - 10.1016/j.ijid.2023.11.026

DO - 10.1016/j.ijid.2023.11.026

M3 - Journal article

C2 - 38016502

AN - SCOPUS:85182501181

VL - 139

SP - 41

EP - 49

JO - International Journal of Infectious Diseases

JF - International Journal of Infectious Diseases

SN - 1201-9712

ER -

ID: 380698072