Five-year surveillance of molecular markers of Plasmodium falciparum antimalarial drug resistance in Korogwe District, Tanzania: accumulation of the 581G mutation in the P. falciparum dihydropteroate synthase gene

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Five-year surveillance of molecular markers of Plasmodium falciparum antimalarial drug resistance in Korogwe District, Tanzania: accumulation of the 581G mutation in the P. falciparum dihydropteroate synthase gene. / Alifrangis, Michael; Lusingu, John P; Mmbando, Bruno; Dalgaard, Michael B; Vestergaard, Lasse S; Ishengoma, Deus; Khalil, Insaf F; Theander, Thor G; Lemnge, Martha M; Bygbjerg, Ib C.

In: American Journal of Tropical Medicine and Hygiene, Vol. 80, No. 4, 2009, p. 523-7.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Alifrangis, M, Lusingu, JP, Mmbando, B, Dalgaard, MB, Vestergaard, LS, Ishengoma, D, Khalil, IF, Theander, TG, Lemnge, MM & Bygbjerg, IC 2009, 'Five-year surveillance of molecular markers of Plasmodium falciparum antimalarial drug resistance in Korogwe District, Tanzania: accumulation of the 581G mutation in the P. falciparum dihydropteroate synthase gene', American Journal of Tropical Medicine and Hygiene, vol. 80, no. 4, pp. 523-7.

APA

Alifrangis, M., Lusingu, J. P., Mmbando, B., Dalgaard, M. B., Vestergaard, L. S., Ishengoma, D., Khalil, I. F., Theander, T. G., Lemnge, M. M., & Bygbjerg, I. C. (2009). Five-year surveillance of molecular markers of Plasmodium falciparum antimalarial drug resistance in Korogwe District, Tanzania: accumulation of the 581G mutation in the P. falciparum dihydropteroate synthase gene. American Journal of Tropical Medicine and Hygiene, 80(4), 523-7.

Vancouver

Alifrangis M, Lusingu JP, Mmbando B, Dalgaard MB, Vestergaard LS, Ishengoma D et al. Five-year surveillance of molecular markers of Plasmodium falciparum antimalarial drug resistance in Korogwe District, Tanzania: accumulation of the 581G mutation in the P. falciparum dihydropteroate synthase gene. American Journal of Tropical Medicine and Hygiene. 2009;80(4):523-7.

Author

Alifrangis, Michael ; Lusingu, John P ; Mmbando, Bruno ; Dalgaard, Michael B ; Vestergaard, Lasse S ; Ishengoma, Deus ; Khalil, Insaf F ; Theander, Thor G ; Lemnge, Martha M ; Bygbjerg, Ib C. / Five-year surveillance of molecular markers of Plasmodium falciparum antimalarial drug resistance in Korogwe District, Tanzania: accumulation of the 581G mutation in the P. falciparum dihydropteroate synthase gene. In: American Journal of Tropical Medicine and Hygiene. 2009 ; Vol. 80, No. 4. pp. 523-7.

Bibtex

@article{d082254064b211de8bc9000ea68e967b,
title = "Five-year surveillance of molecular markers of Plasmodium falciparum antimalarial drug resistance in Korogwe District, Tanzania: accumulation of the 581G mutation in the P. falciparum dihydropteroate synthase gene",
abstract = "In January 2007, Tanzania replaced sulfadoxine-pyrimethamine (SP) with artemether-lumefantrine for treatment of uncomplicated malaria. This study examined the impact of widespread SP use on molecular markers of Plasmodium falciparum drug resistance in blood samples from persons living in two villages in Korogwe District, Tanzania, from 2003 through 2007. The prevalence of the P. falciparum dihydropteroate synthase (Pfdhps) gene 581G mutation increased from 12% in 2003 to 56% in 2007 (P < 0.001), resulting in an increase in the triple mutant Pfdhps haplotype SGEGA from 8% to 32% (P < 0.001). In contrast, the chloroquine-sensitive P. falciparum chloroquine resistance transporter (Pfcrt) CVMNK haplotype increased from 6% to 30% (P < 0.001). The dramatic increase of the triple Pfdhps mutant SGEGA haplotype may endanger the continued use of SP for intermittent presumptive treatment of pregnant women (IPTp). Further studies are needed to determine the importance of Pfdhps SGEGA haplotype parasites on the efficacy of SP for IPTp.",
author = "Michael Alifrangis and Lusingu, {John P} and Bruno Mmbando and Dalgaard, {Michael B} and Vestergaard, {Lasse S} and Deus Ishengoma and Khalil, {Insaf F} and Theander, {Thor G} and Lemnge, {Martha M} and Bygbjerg, {Ib C}",
note = "Keywords: Animals; Antimalarials; Dihydropteroate Synthase; Drug Resistance; Genetic Markers; Haplotypes; Humans; Malaria, Falciparum; Mutation; Plasmodium falciparum; Population Surveillance; Tanzania; Time",
year = "2009",
language = "English",
volume = "80",
pages = "523--7",
journal = "Journal. National Malaria Society",
issn = "0002-9637",
publisher = "American Society of Tropical Medicine and Hygiene",
number = "4",

}

RIS

TY - JOUR

T1 - Five-year surveillance of molecular markers of Plasmodium falciparum antimalarial drug resistance in Korogwe District, Tanzania: accumulation of the 581G mutation in the P. falciparum dihydropteroate synthase gene

AU - Alifrangis, Michael

AU - Lusingu, John P

AU - Mmbando, Bruno

AU - Dalgaard, Michael B

AU - Vestergaard, Lasse S

AU - Ishengoma, Deus

AU - Khalil, Insaf F

AU - Theander, Thor G

AU - Lemnge, Martha M

AU - Bygbjerg, Ib C

N1 - Keywords: Animals; Antimalarials; Dihydropteroate Synthase; Drug Resistance; Genetic Markers; Haplotypes; Humans; Malaria, Falciparum; Mutation; Plasmodium falciparum; Population Surveillance; Tanzania; Time

PY - 2009

Y1 - 2009

N2 - In January 2007, Tanzania replaced sulfadoxine-pyrimethamine (SP) with artemether-lumefantrine for treatment of uncomplicated malaria. This study examined the impact of widespread SP use on molecular markers of Plasmodium falciparum drug resistance in blood samples from persons living in two villages in Korogwe District, Tanzania, from 2003 through 2007. The prevalence of the P. falciparum dihydropteroate synthase (Pfdhps) gene 581G mutation increased from 12% in 2003 to 56% in 2007 (P < 0.001), resulting in an increase in the triple mutant Pfdhps haplotype SGEGA from 8% to 32% (P < 0.001). In contrast, the chloroquine-sensitive P. falciparum chloroquine resistance transporter (Pfcrt) CVMNK haplotype increased from 6% to 30% (P < 0.001). The dramatic increase of the triple Pfdhps mutant SGEGA haplotype may endanger the continued use of SP for intermittent presumptive treatment of pregnant women (IPTp). Further studies are needed to determine the importance of Pfdhps SGEGA haplotype parasites on the efficacy of SP for IPTp.

AB - In January 2007, Tanzania replaced sulfadoxine-pyrimethamine (SP) with artemether-lumefantrine for treatment of uncomplicated malaria. This study examined the impact of widespread SP use on molecular markers of Plasmodium falciparum drug resistance in blood samples from persons living in two villages in Korogwe District, Tanzania, from 2003 through 2007. The prevalence of the P. falciparum dihydropteroate synthase (Pfdhps) gene 581G mutation increased from 12% in 2003 to 56% in 2007 (P < 0.001), resulting in an increase in the triple mutant Pfdhps haplotype SGEGA from 8% to 32% (P < 0.001). In contrast, the chloroquine-sensitive P. falciparum chloroquine resistance transporter (Pfcrt) CVMNK haplotype increased from 6% to 30% (P < 0.001). The dramatic increase of the triple Pfdhps mutant SGEGA haplotype may endanger the continued use of SP for intermittent presumptive treatment of pregnant women (IPTp). Further studies are needed to determine the importance of Pfdhps SGEGA haplotype parasites on the efficacy of SP for IPTp.

M3 - Journal article

C2 - 19346369

VL - 80

SP - 523

EP - 527

JO - Journal. National Malaria Society

JF - Journal. National Malaria Society

SN - 0002-9637

IS - 4

ER -

ID: 12869804