Expression of single-domain soluble and disulfide-folded PfEMP1 antigens in the Escherichia coli SHuffle expression system

Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review

Standard

Expression of single-domain soluble and disulfide-folded PfEMP1 antigens in the Escherichia coli SHuffle expression system. / Quintana, Maria Del Pilar.

Malaria Immunology: Targeting the Surface of Infected Erythrocytes. Vol. 2470 Humana Press, 2022. p. 273-282 (Methods in molecular biology (Clifton, N.J.)).

Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review

Harvard

Quintana, MDP 2022, Expression of single-domain soluble and disulfide-folded PfEMP1 antigens in the Escherichia coli SHuffle expression system. in Malaria Immunology: Targeting the Surface of Infected Erythrocytes. vol. 2470, Humana Press, Methods in molecular biology (Clifton, N.J.), pp. 273-282. https://doi.org/10.1007/978-1-0716-2189-9_20

APA

Quintana, M. D. P. (2022). Expression of single-domain soluble and disulfide-folded PfEMP1 antigens in the Escherichia coli SHuffle expression system. In Malaria Immunology: Targeting the Surface of Infected Erythrocytes (Vol. 2470, pp. 273-282). Humana Press. Methods in molecular biology (Clifton, N.J.) https://doi.org/10.1007/978-1-0716-2189-9_20

Vancouver

Quintana MDP. Expression of single-domain soluble and disulfide-folded PfEMP1 antigens in the Escherichia coli SHuffle expression system. In Malaria Immunology: Targeting the Surface of Infected Erythrocytes. Vol. 2470. Humana Press. 2022. p. 273-282. (Methods in molecular biology (Clifton, N.J.)). https://doi.org/10.1007/978-1-0716-2189-9_20

Author

Quintana, Maria Del Pilar. / Expression of single-domain soluble and disulfide-folded PfEMP1 antigens in the Escherichia coli SHuffle expression system. Malaria Immunology: Targeting the Surface of Infected Erythrocytes. Vol. 2470 Humana Press, 2022. pp. 273-282 (Methods in molecular biology (Clifton, N.J.)).

Bibtex

@inbook{194c1401a7374c5087b83eb83cc6f26f,
title = "Expression of single-domain soluble and disulfide-folded PfEMP1 antigens in the Escherichia coli SHuffle expression system",
abstract = "The genome of Plasmodium falciparum has an A/T content of around 81%. This, together with a high cysteine content and the high molecular weight of several proteins, make the expression of recombinant parasite proteins in heterologous systems challenging. P. falciparum erythrocyte membrane protein 1 (PfEMP1) is a family of proteins composed of several Duffy-binding like (DBL) and cysteine-rich inter-domain region (CIDR) domains involved in cytoadhesion to human host receptors and development of severe malaria. Expression of correctly folded single- and multiple-domain PfEMP1 fragment regions containing cysteines forming disulfide bonds, remains particularly difficult. Nevertheless, expression of single DBL and CIDR domains has been successful and this protocol describes the expression and purification of single-domain soluble PfEMP1 fragments using the Escherichia coli SHuffle expression system.",
keywords = "Antigens, Protozoan, Cysteine/metabolism, Disulfides/metabolism, Erythrocytes/metabolism, Escherichia coli/genetics, Humans, Malaria, Falciparum, Plasmodium falciparum/metabolism, Protozoan Proteins/metabolism, Recombinant Proteins/genetics",
author = "Quintana, {Maria Del Pilar}",
note = "{\textcopyright} 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2022",
doi = "10.1007/978-1-0716-2189-9_20",
language = "English",
isbn = "978-1-0716-2188-2",
volume = "2470",
series = "Methods in molecular biology (Clifton, N.J.)",
publisher = "Humana Press",
pages = "273--282",
booktitle = "Malaria Immunology",
address = "United States",

}

RIS

TY - CHAP

T1 - Expression of single-domain soluble and disulfide-folded PfEMP1 antigens in the Escherichia coli SHuffle expression system

AU - Quintana, Maria Del Pilar

N1 - © 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

PY - 2022

Y1 - 2022

N2 - The genome of Plasmodium falciparum has an A/T content of around 81%. This, together with a high cysteine content and the high molecular weight of several proteins, make the expression of recombinant parasite proteins in heterologous systems challenging. P. falciparum erythrocyte membrane protein 1 (PfEMP1) is a family of proteins composed of several Duffy-binding like (DBL) and cysteine-rich inter-domain region (CIDR) domains involved in cytoadhesion to human host receptors and development of severe malaria. Expression of correctly folded single- and multiple-domain PfEMP1 fragment regions containing cysteines forming disulfide bonds, remains particularly difficult. Nevertheless, expression of single DBL and CIDR domains has been successful and this protocol describes the expression and purification of single-domain soluble PfEMP1 fragments using the Escherichia coli SHuffle expression system.

AB - The genome of Plasmodium falciparum has an A/T content of around 81%. This, together with a high cysteine content and the high molecular weight of several proteins, make the expression of recombinant parasite proteins in heterologous systems challenging. P. falciparum erythrocyte membrane protein 1 (PfEMP1) is a family of proteins composed of several Duffy-binding like (DBL) and cysteine-rich inter-domain region (CIDR) domains involved in cytoadhesion to human host receptors and development of severe malaria. Expression of correctly folded single- and multiple-domain PfEMP1 fragment regions containing cysteines forming disulfide bonds, remains particularly difficult. Nevertheless, expression of single DBL and CIDR domains has been successful and this protocol describes the expression and purification of single-domain soluble PfEMP1 fragments using the Escherichia coli SHuffle expression system.

KW - Antigens, Protozoan

KW - Cysteine/metabolism

KW - Disulfides/metabolism

KW - Erythrocytes/metabolism

KW - Escherichia coli/genetics

KW - Humans

KW - Malaria, Falciparum

KW - Plasmodium falciparum/metabolism

KW - Protozoan Proteins/metabolism

KW - Recombinant Proteins/genetics

U2 - 10.1007/978-1-0716-2189-9_20

DO - 10.1007/978-1-0716-2189-9_20

M3 - Book chapter

C2 - 35881352

SN - 978-1-0716-2188-2

VL - 2470

T3 - Methods in molecular biology (Clifton, N.J.)

SP - 273

EP - 282

BT - Malaria Immunology

PB - Humana Press

ER -

ID: 327471856