Epitopes of anti-RIFIN antibodies and characterization of rif-expressing Plasmodium falciparum parasites by RNA sequencing
Research output: Contribution to journal › Journal article › Research › peer-review
Standard
Epitopes of anti-RIFIN antibodies and characterization of rif-expressing Plasmodium falciparum parasites by RNA sequencing. / Ch'Ng, Jun Hong; Sirel, Madle; Zandian, Arash; Del Pilar Quintana, Maria; Chun Leung Chan, Sherwin; Moll, Kirsten; Tellgren-Roth, Asa; Nilsson, Ingmarie; Nilsson, Peter; Qundos, Ulrika; Wahlgren, Mats.
In: Scientific Reports, Vol. 7, 43190, 24.02.2017.Research output: Contribution to journal › Journal article › Research › peer-review
Harvard
APA
Vancouver
Author
Bibtex
}
RIS
TY - JOUR
T1 - Epitopes of anti-RIFIN antibodies and characterization of rif-expressing Plasmodium falciparum parasites by RNA sequencing
AU - Ch'Ng, Jun Hong
AU - Sirel, Madle
AU - Zandian, Arash
AU - Del Pilar Quintana, Maria
AU - Chun Leung Chan, Sherwin
AU - Moll, Kirsten
AU - Tellgren-Roth, Asa
AU - Nilsson, Ingmarie
AU - Nilsson, Peter
AU - Qundos, Ulrika
AU - Wahlgren, Mats
PY - 2017/2/24
Y1 - 2017/2/24
N2 - Variable surface antigens of Plasmodium falciparum have been a major research focus since they facilitate parasite sequestration and give rise to deadly malaria complications. Coupled with its potential use as a vaccine candidate, the recent suggestion that the repetitive interspersed families of polypeptides (RIFINs) mediate blood group A rosetting and influence blood group distribution has raised the research profile of these adhesins. Nevertheless, detailed investigations into the functions of this highly diverse multigene family remain hampered by the limited number of validated reagents. In this study, we assess the specificities of three promising polyclonal anti-RIFIN antibodies that were IgG-purified from sera of immunized animals. Their epitope regions were mapped using a 175,000-peptide microarray holding overlapping peptides of the P. falciparum variable surface antigens. Through immunoblotting and immunofluorescence imaging, we show that different antibodies give varying results in different applications/assays. Finally, we authenticate the antibody-based detection of RIFINs in two previously uncharacterized non-rosetting parasite lines by identifying the dominant rif transcripts using RNA sequencing.
AB - Variable surface antigens of Plasmodium falciparum have been a major research focus since they facilitate parasite sequestration and give rise to deadly malaria complications. Coupled with its potential use as a vaccine candidate, the recent suggestion that the repetitive interspersed families of polypeptides (RIFINs) mediate blood group A rosetting and influence blood group distribution has raised the research profile of these adhesins. Nevertheless, detailed investigations into the functions of this highly diverse multigene family remain hampered by the limited number of validated reagents. In this study, we assess the specificities of three promising polyclonal anti-RIFIN antibodies that were IgG-purified from sera of immunized animals. Their epitope regions were mapped using a 175,000-peptide microarray holding overlapping peptides of the P. falciparum variable surface antigens. Through immunoblotting and immunofluorescence imaging, we show that different antibodies give varying results in different applications/assays. Finally, we authenticate the antibody-based detection of RIFINs in two previously uncharacterized non-rosetting parasite lines by identifying the dominant rif transcripts using RNA sequencing.
U2 - 10.1038/srep43190
DO - 10.1038/srep43190
M3 - Journal article
C2 - 28233866
AN - SCOPUS:85013755282
VL - 7
JO - Scientific Reports
JF - Scientific Reports
SN - 2045-2322
M1 - 43190
ER -
ID: 197729466