Tumour necrosis factor and interleukin-6 production induced by components associated with merozoite proteins of Plasmodium falciparum
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Tumour necrosis factor and interleukin-6 production induced by components associated with merozoite proteins of Plasmodium falciparum. / Jakobsen, P H; Moon, R; Ridley, R G; Bate, C A; Taverne, J; Hansen, M B; Takacs, B; Playfair, J H; McBride, J S.
In: Parasite Immunology, Vol. 15, No. 4, 1993, p. 229-37.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Tumour necrosis factor and interleukin-6 production induced by components associated with merozoite proteins of Plasmodium falciparum
AU - Jakobsen, P H
AU - Moon, R
AU - Ridley, R G
AU - Bate, C A
AU - Taverne, J
AU - Hansen, M B
AU - Takacs, B
AU - Playfair, J H
AU - McBride, J S
N1 - Keywords: Animals; Antibodies, Monoclonal; Antibodies, Protozoan; Antigens, Protozoan; Antigens, Surface; Blotting, Western; Cross Reactions; Electrophoresis, Polyacrylamide Gel; Enzyme-Linked Immunosorbent Assay; Fluorescent Antibody Technique; Humans; Immune Sera; Immunoelectrophoresis, Two-Dimensional; Interleukin-6; Malaria, Falciparum; Merozoite Surface Protein 1; Plasmodium falciparum; Plasmodium yoelii; Protein Precursors; Protozoan Proteins; Tumor Necrosis Factor-alpha
PY - 1993
Y1 - 1993
N2 - P. falciparum merozoite antigens, merozoite surface protein-1 (MSP-1) and rhoptry associated protein-1 (RAP-1), were shown to be liberated into the supernatant of in vitro parasite cultures and to be included in the endotoxin-like exoantigen complex, previously designated Ag7. Material affinity purified from culture supernatants, using immobilized monoclonal antibodies specific for RAP-1 or MSP-1, stimulated normal human mononuclear cells to produce TNF and IL-6 in vitro. However, stimulation of TNF was absent, and that of IL-6 was reduced, when the antigens were purified from detergent extracts of infected erythrocytes. These results indicate that the RAP-1 and MSP-1 proteins themselves do not stimulate the production of TNF. Instead, other components associating with these exoantigens may be responsible for the TNF production. Mouse antisera blocking TNF production stimulated by P. yoelii exoantigens also blocked TNF production stimulated by material affinity purified from P. falciparum culture supernatants using RAP-1 specific monoclonal antibody, indicating the conserved structure of the TNF inducing component.
AB - P. falciparum merozoite antigens, merozoite surface protein-1 (MSP-1) and rhoptry associated protein-1 (RAP-1), were shown to be liberated into the supernatant of in vitro parasite cultures and to be included in the endotoxin-like exoantigen complex, previously designated Ag7. Material affinity purified from culture supernatants, using immobilized monoclonal antibodies specific for RAP-1 or MSP-1, stimulated normal human mononuclear cells to produce TNF and IL-6 in vitro. However, stimulation of TNF was absent, and that of IL-6 was reduced, when the antigens were purified from detergent extracts of infected erythrocytes. These results indicate that the RAP-1 and MSP-1 proteins themselves do not stimulate the production of TNF. Instead, other components associating with these exoantigens may be responsible for the TNF production. Mouse antisera blocking TNF production stimulated by P. yoelii exoantigens also blocked TNF production stimulated by material affinity purified from P. falciparum culture supernatants using RAP-1 specific monoclonal antibody, indicating the conserved structure of the TNF inducing component.
M3 - Journal article
C2 - 8506119
VL - 15
SP - 229
EP - 237
JO - Parasite Immunology
JF - Parasite Immunology
SN - 0141-9838
IS - 4
ER -
ID: 18153077