Suitability of IgG responses to multiple Plasmodium falciparum antigens as markers of transmission intensity and pattern

Research output: Contribution to journal › Journal article › Research › peer-review

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Suitability of IgG responses to multiple Plasmodium falciparum antigens as markers of transmission intensity and pattern. / Kyei-Baafour, Eric; Oppong, Mavis; Kusi, Kwadwo Asamoah; Frempong, Abena Fremaah; Aculley, Belinda; Arthur, Fareed K N; Tiendrebeogo, Regis Wendpayangde; Singh, Susheel K; Theisen, Michael; Kweku, Margaret; Adu, Bright; Hviid, Lars; Ofori, Michael Fokuo.

In: PLoS ONE, Vol. 16, No. 4, e0249936, 2021.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Kyei-Baafour, E, Oppong, M, Kusi, KA, Frempong, AF, Aculley, B, Arthur, FKN, Tiendrebeogo, RW, Singh, SK, Theisen, M, Kweku, M, Adu, B, Hviid, L & Ofori, MF 2021, 'Suitability of IgG responses to multiple Plasmodium falciparum antigens as markers of transmission intensity and pattern', PLoS ONE, vol. 16, no. 4, e0249936. https://doi.org/10.1371/journal.pone.0249936

APA

Kyei-Baafour, E., Oppong, M., Kusi, K. A., Frempong, A. F., Aculley, B., Arthur, F. K. N., Tiendrebeogo, R. W., Singh, S. K., Theisen, M., Kweku, M., Adu, B., Hviid, L., & Ofori, M. F. (2021). Suitability of IgG responses to multiple Plasmodium falciparum antigens as markers of transmission intensity and pattern. PLoS ONE, 16(4), [e0249936]. https://doi.org/10.1371/journal.pone.0249936

Vancouver

Kyei-Baafour E, Oppong M, Kusi KA, Frempong AF, Aculley B, Arthur FKN et al. Suitability of IgG responses to multiple Plasmodium falciparum antigens as markers of transmission intensity and pattern. PLoS ONE. 2021;16(4). e0249936. https://doi.org/10.1371/journal.pone.0249936

Author

Kyei-Baafour, Eric ; Oppong, Mavis ; Kusi, Kwadwo Asamoah ; Frempong, Abena Fremaah ; Aculley, Belinda ; Arthur, Fareed K N ; Tiendrebeogo, Regis Wendpayangde ; Singh, Susheel K ; Theisen, Michael ; Kweku, Margaret ; Adu, Bright ; Hviid, Lars ; Ofori, Michael Fokuo. / Suitability of IgG responses to multiple Plasmodium falciparum antigens as markers of transmission intensity and pattern. In: PLoS ONE. 2021 ; Vol. 16, No. 4.

Bibtex

@article{1b8fc3b7181a44279928cd0353409f7f,
title = "Suitability of IgG responses to multiple Plasmodium falciparum antigens as markers of transmission intensity and pattern",
abstract = "Detection of antibody reactivity to appropriate, specific parasite antigens may constitute a sensitive and cost-effective alternative to current tools to monitor malaria transmission across different endemicity settings. This study aimed to determine the suitability of IgG responses to a number of P. falciparum antigens as markers of transmission intensity and pattern. Antibody responses to multiple malaria antigens were determined in 905 participants aged 1-12 years from three districts with low (Keta), medium (Hohoe) and high (Krachi) transmission intensity in the Volta region of Ghana. Blood film microscopy slides and dry blood spots (DBS) were obtained for parasitaemia detection and antibody measurement, respectively. Sera were eluted from DBS and levels of IgG specific for 10 malaria antigens determined by a multiplex assay. Results were compared within and among the districts. Total IgG responses to MSPDBL1, MSPDBLLeucine, MSP2-FC27, RAMA, and PfRh2a and PfRh2b were higher in Krachi than in Hohoe and Keta. Seroprevalence of IgG specific for MSPDBLLeucine, RON4, and PfRh2b were also highest in Krachi. Responses to RALP-1, PfRh2a and PfRh2b were associated with patent but asymptomatic parasitaemia in Keta, while responses to MSPDBL1, MSPDBLLeucine, MSP2-FC27, RAMA, Rh2-2030, and PfRh2b were associated with parasite carriage in Hohoe, but not in Krachi. Using ROC analysis, only PfRh2b was found to predict patent, but asymptomatic, parasitaemia in Keta and Hohoe. Antibody breadth correlated positively with age (r = 0.29, p<0.0001) and parasitaemia (β = 3.91; CI = 1.53 to 6.29), and medium to high transmission (p<0.0001). Our findings suggest differences in malaria-specific antibody responses across the three transmission zones and that PfRh2b has potential as a marker of malaria transmission intensity and pattern. This could have implications for malaria control programs and vaccine trials.",
author = "Eric Kyei-Baafour and Mavis Oppong and Kusi, {Kwadwo Asamoah} and Frempong, {Abena Fremaah} and Belinda Aculley and Arthur, {Fareed K N} and Tiendrebeogo, {Regis Wendpayangde} and Singh, {Susheel K} and Michael Theisen and Margaret Kweku and Bright Adu and Lars Hviid and Ofori, {Michael Fokuo}",
year = "2021",
doi = "10.1371/journal.pone.0249936",
language = "English",
volume = "16",
journal = "PLoS ONE",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "4",

}

RIS

TY - JOUR

T1 - Suitability of IgG responses to multiple Plasmodium falciparum antigens as markers of transmission intensity and pattern

AU - Kyei-Baafour, Eric

AU - Oppong, Mavis

AU - Kusi, Kwadwo Asamoah

AU - Frempong, Abena Fremaah

AU - Aculley, Belinda

AU - Arthur, Fareed K N

AU - Tiendrebeogo, Regis Wendpayangde

AU - Singh, Susheel K

AU - Theisen, Michael

AU - Kweku, Margaret

AU - Adu, Bright

AU - Hviid, Lars

AU - Ofori, Michael Fokuo

PY - 2021

Y1 - 2021

N2 - Detection of antibody reactivity to appropriate, specific parasite antigens may constitute a sensitive and cost-effective alternative to current tools to monitor malaria transmission across different endemicity settings. This study aimed to determine the suitability of IgG responses to a number of P. falciparum antigens as markers of transmission intensity and pattern. Antibody responses to multiple malaria antigens were determined in 905 participants aged 1-12 years from three districts with low (Keta), medium (Hohoe) and high (Krachi) transmission intensity in the Volta region of Ghana. Blood film microscopy slides and dry blood spots (DBS) were obtained for parasitaemia detection and antibody measurement, respectively. Sera were eluted from DBS and levels of IgG specific for 10 malaria antigens determined by a multiplex assay. Results were compared within and among the districts. Total IgG responses to MSPDBL1, MSPDBLLeucine, MSP2-FC27, RAMA, and PfRh2a and PfRh2b were higher in Krachi than in Hohoe and Keta. Seroprevalence of IgG specific for MSPDBLLeucine, RON4, and PfRh2b were also highest in Krachi. Responses to RALP-1, PfRh2a and PfRh2b were associated with patent but asymptomatic parasitaemia in Keta, while responses to MSPDBL1, MSPDBLLeucine, MSP2-FC27, RAMA, Rh2-2030, and PfRh2b were associated with parasite carriage in Hohoe, but not in Krachi. Using ROC analysis, only PfRh2b was found to predict patent, but asymptomatic, parasitaemia in Keta and Hohoe. Antibody breadth correlated positively with age (r = 0.29, p<0.0001) and parasitaemia (β = 3.91; CI = 1.53 to 6.29), and medium to high transmission (p<0.0001). Our findings suggest differences in malaria-specific antibody responses across the three transmission zones and that PfRh2b has potential as a marker of malaria transmission intensity and pattern. This could have implications for malaria control programs and vaccine trials.

AB - Detection of antibody reactivity to appropriate, specific parasite antigens may constitute a sensitive and cost-effective alternative to current tools to monitor malaria transmission across different endemicity settings. This study aimed to determine the suitability of IgG responses to a number of P. falciparum antigens as markers of transmission intensity and pattern. Antibody responses to multiple malaria antigens were determined in 905 participants aged 1-12 years from three districts with low (Keta), medium (Hohoe) and high (Krachi) transmission intensity in the Volta region of Ghana. Blood film microscopy slides and dry blood spots (DBS) were obtained for parasitaemia detection and antibody measurement, respectively. Sera were eluted from DBS and levels of IgG specific for 10 malaria antigens determined by a multiplex assay. Results were compared within and among the districts. Total IgG responses to MSPDBL1, MSPDBLLeucine, MSP2-FC27, RAMA, and PfRh2a and PfRh2b were higher in Krachi than in Hohoe and Keta. Seroprevalence of IgG specific for MSPDBLLeucine, RON4, and PfRh2b were also highest in Krachi. Responses to RALP-1, PfRh2a and PfRh2b were associated with patent but asymptomatic parasitaemia in Keta, while responses to MSPDBL1, MSPDBLLeucine, MSP2-FC27, RAMA, Rh2-2030, and PfRh2b were associated with parasite carriage in Hohoe, but not in Krachi. Using ROC analysis, only PfRh2b was found to predict patent, but asymptomatic, parasitaemia in Keta and Hohoe. Antibody breadth correlated positively with age (r = 0.29, p<0.0001) and parasitaemia (β = 3.91; CI = 1.53 to 6.29), and medium to high transmission (p<0.0001). Our findings suggest differences in malaria-specific antibody responses across the three transmission zones and that PfRh2b has potential as a marker of malaria transmission intensity and pattern. This could have implications for malaria control programs and vaccine trials.

U2 - 10.1371/journal.pone.0249936

DO - 10.1371/journal.pone.0249936

M3 - Journal article

C2 - 33886601

VL - 16

JO - PLoS ONE

JF - PLoS ONE

SN - 1932-6203

IS - 4

M1 - e0249936

ER -

ID: 260410147