Selection of antimalarial drug resistance after intermittent preventive treatment of infants and children (IPTi/c) in Senegal
Research output: Contribution to journal › Journal article › Research › peer-review
Standard
Selection of antimalarial drug resistance after intermittent preventive treatment of infants and children (IPTi/c) in Senegal. / Ndiaye, Magatte; Tine, Roger; Faye, Babacar; Ndiaye, Jean Louis; Lo, Ami Colle; Sylla, Khadime; Abiola, Annie; Dieng, Yémou; Ndiaye, Daouda; Hallett, Rachel; Gaye, Oumar; Alifrangis, Michael.
In: American Journal of Tropical Medicine and Hygiene, Vol. 88, No. 6, 06.2013, p. 1124-9.Research output: Contribution to journal › Journal article › Research › peer-review
Harvard
APA
Vancouver
Author
Bibtex
}
RIS
TY - JOUR
T1 - Selection of antimalarial drug resistance after intermittent preventive treatment of infants and children (IPTi/c) in Senegal
AU - Ndiaye, Magatte
AU - Tine, Roger
AU - Faye, Babacar
AU - Ndiaye, Jean Louis
AU - Lo, Ami Colle
AU - Sylla, Khadime
AU - Abiola, Annie
AU - Dieng, Yémou
AU - Ndiaye, Daouda
AU - Hallett, Rachel
AU - Gaye, Oumar
AU - Alifrangis, Michael
PY - 2013/6
Y1 - 2013/6
N2 - Abstract. Our study investigated the possible impact of SP-IPT given to infants and children on the prevalence of SP-resistant haplotypes in the Plasmodium falciparum genes Pfdhfr and Pfdhps, comparing sites with and without IPTi/c. P. falciparum positive samples (N = 352) collected from children <5 years were analyzed to determine the prevalence of SP resistance-related haplotypes by nested PCR followed by sequence-specific oligonucleotide probe-enzyme-linked immunosorbent assay. The prevalence of the Pfdhfr triple mutant haplotype (CIRN) increased in both groups, but only significantly in the IPTi/c group from 41% to 65% in 2011 (P = 0.005). Conversely, the Pfdhps 437G mutation decreased in both groups from 44.6% to 28.6% (P = 0.07) and from 66.7% to 47.5% (P = 0.02) between 2010 and 2011 in the control and the IPTi/c groups, respectively. A weak trend for decreasing prevalence of quadruple mutants (triple Pfdhfr + Pfdhps 437G) was noted in both groups (P = 0.15 and P = 0.34). During the two cross-sectional surveys some significant changes were observed in the SP resistance-related genes.
AB - Abstract. Our study investigated the possible impact of SP-IPT given to infants and children on the prevalence of SP-resistant haplotypes in the Plasmodium falciparum genes Pfdhfr and Pfdhps, comparing sites with and without IPTi/c. P. falciparum positive samples (N = 352) collected from children <5 years were analyzed to determine the prevalence of SP resistance-related haplotypes by nested PCR followed by sequence-specific oligonucleotide probe-enzyme-linked immunosorbent assay. The prevalence of the Pfdhfr triple mutant haplotype (CIRN) increased in both groups, but only significantly in the IPTi/c group from 41% to 65% in 2011 (P = 0.005). Conversely, the Pfdhps 437G mutation decreased in both groups from 44.6% to 28.6% (P = 0.07) and from 66.7% to 47.5% (P = 0.02) between 2010 and 2011 in the control and the IPTi/c groups, respectively. A weak trend for decreasing prevalence of quadruple mutants (triple Pfdhfr + Pfdhps 437G) was noted in both groups (P = 0.15 and P = 0.34). During the two cross-sectional surveys some significant changes were observed in the SP resistance-related genes.
U2 - 10.4269/ajtmh.12-0739
DO - 10.4269/ajtmh.12-0739
M3 - Journal article
C2 - 23589534
VL - 88
SP - 1124
EP - 1129
JO - Journal. National Malaria Society
JF - Journal. National Malaria Society
SN - 0002-9637
IS - 6
ER -
ID: 46152465