Rainbow trout (Oncorhynchus mykiss) immune response towards a recombinant vaccine targeting the parasitic ciliate Ichthyophthirius multifiliis
Research output: Contribution to journal › Journal article › Research › peer-review
Standard
Rainbow trout (Oncorhynchus mykiss) immune response towards a recombinant vaccine targeting the parasitic ciliate Ichthyophthirius multifiliis. / Jørgensen, Louise von Gersdorff; Kania, Per Walter; Rasmussen, Karina Juhl; Mattsson, Andreas ; Schmidt, Jacob Günther; Al-Jubury, Azmi; Pedersen, Adam Frederik Sander; Salanti, Ali; Buchmann, Kurt.
In: Journal of Fish Diseases, Vol. 40, No. 12, 12.2017, p. 1815–1821.Research output: Contribution to journal › Journal article › Research › peer-review
Harvard
APA
Vancouver
Author
Bibtex
}
RIS
TY - JOUR
T1 - Rainbow trout (Oncorhynchus mykiss) immune response towards a recombinant vaccine targeting the parasitic ciliate Ichthyophthirius multifiliis
AU - Jørgensen, Louise von Gersdorff
AU - Kania, Per Walter
AU - Rasmussen, Karina Juhl
AU - Mattsson, Andreas
AU - Schmidt, Jacob Günther
AU - Al-Jubury, Azmi
AU - Pedersen, Adam Frederik Sander
AU - Salanti, Ali
AU - Buchmann, Kurt
PY - 2017/12
Y1 - 2017/12
N2 - The protective effect in rainbow trout (Oncorhynchus mykiss) of an experimental subunit vaccine targeting antigens in the parasite Ichthyophthirius multifiliis has been evaluated and compared to effects elicited by a classical parasite homogenate vaccine. Three recombinant parasite proteins (two produced in E. coli and one in insect cells) were combined and injected i.p., and subsequently, protection and antibody responses were analysed. Both the experimental and the benchmark vaccine induced partial but significant protection against I. multifiliis when compared to control fish. Specific antibody responses of vaccinated trout (subunit vaccine) were raised against one neurohypophysial n-terminal domain protein #10 of three recombinant proteins, whereas the benchmark vaccine group showed specific antibody production against all three recombinant proteins. The immunogenic parasite protein #10 may be a potential vaccine candidate supplementing the protective I-antigen in future vaccine trials.
AB - The protective effect in rainbow trout (Oncorhynchus mykiss) of an experimental subunit vaccine targeting antigens in the parasite Ichthyophthirius multifiliis has been evaluated and compared to effects elicited by a classical parasite homogenate vaccine. Three recombinant parasite proteins (two produced in E. coli and one in insect cells) were combined and injected i.p., and subsequently, protection and antibody responses were analysed. Both the experimental and the benchmark vaccine induced partial but significant protection against I. multifiliis when compared to control fish. Specific antibody responses of vaccinated trout (subunit vaccine) were raised against one neurohypophysial n-terminal domain protein #10 of three recombinant proteins, whereas the benchmark vaccine group showed specific antibody production against all three recombinant proteins. The immunogenic parasite protein #10 may be a potential vaccine candidate supplementing the protective I-antigen in future vaccine trials.
U2 - 10.1111/jfd.12653
DO - 10.1111/jfd.12653
M3 - Journal article
C2 - 28548690
VL - 40
SP - 1815
EP - 1821
JO - Journal of Fish Diseases
JF - Journal of Fish Diseases
SN - 0140-7775
IS - 12
ER -
ID: 178690233