Production of PfEMP1-specific mouse monoclonal antibodies

Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review

Standard

Production of PfEMP1-specific mouse monoclonal antibodies. / Dalgaard, Nanna; Barfod, Lea.

Malaria Immunology: Targeting the Surface of Infected Erythrocytes. Vol. 2470 Humana Press, 2022. p. 391-405 (Methods in Molecular Biology; No. 2470).

Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review

Harvard

Dalgaard, N & Barfod, L 2022, Production of PfEMP1-specific mouse monoclonal antibodies. in Malaria Immunology: Targeting the Surface of Infected Erythrocytes. vol. 2470, Humana Press, Methods in Molecular Biology, no. 2470, pp. 391-405. https://doi.org/10.1007/978-1-0716-2189-9_29

APA

Dalgaard, N., & Barfod, L. (2022). Production of PfEMP1-specific mouse monoclonal antibodies. In Malaria Immunology: Targeting the Surface of Infected Erythrocytes (Vol. 2470, pp. 391-405). Humana Press. Methods in Molecular Biology No. 2470 https://doi.org/10.1007/978-1-0716-2189-9_29

Vancouver

Dalgaard N, Barfod L. Production of PfEMP1-specific mouse monoclonal antibodies. In Malaria Immunology: Targeting the Surface of Infected Erythrocytes. Vol. 2470. Humana Press. 2022. p. 391-405. (Methods in Molecular Biology; No. 2470). https://doi.org/10.1007/978-1-0716-2189-9_29

Author

Dalgaard, Nanna ; Barfod, Lea. / Production of PfEMP1-specific mouse monoclonal antibodies. Malaria Immunology: Targeting the Surface of Infected Erythrocytes. Vol. 2470 Humana Press, 2022. pp. 391-405 (Methods in Molecular Biology; No. 2470).

Bibtex

@inbook{7790108696854fe2bd3002ed5a2962d4,
title = "Production of PfEMP1-specific mouse monoclonal antibodies",
abstract = "The PfEMP1 family of proteins expressed on the Plasmodium falciparum-infected erythrocyte (IE) surface is the main target of naturally acquired immunity against malaria. Antibodies capable of opsonizing the IEs and blocking the binding between PfEMP1 and human receptors seems to be one of the main protective mechanisms of the naturally acquired immunity. Therefore this family of antigens is intensively studied. Monoclonal antibodies (mAbs) are a very valuable research tool for studying this diverse family of proteins and their interaction with human receptors. As examples, mAbs can be used to identify protective epitopes, epitopes that are targets of cross-reactive antibodies, and the surface expression of specific PfEMP1 variants. Fusing mouse splenocytes with myeloma cells to generate long-lived antibody secreting hybridoma cell lines have been used since the 1970s for the production of mAbs. In this chapter, we describe a simple, reliable, and relatively fast method for producing PfEMP1-specific mAbs from mouse spleen cells using semisolid HAT selection medium.",
keywords = "Animals, Antibodies, Monoclonal/metabolism, Antibodies, Protozoan, Antigens, Protozoan, Epitopes/metabolism, Erythrocytes/metabolism, Humans, Immunosuppressive Agents, Malaria, Falciparum, Mice, Plasmodium falciparum/metabolism, Protozoan Proteins/metabolism",
author = "Nanna Dalgaard and Lea Barfod",
note = "{\textcopyright} 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2022",
doi = "10.1007/978-1-0716-2189-9_29",
language = "English",
isbn = "978-1-0716-2188-2",
volume = "2470",
series = "Methods in Molecular Biology",
publisher = "Humana Press",
number = "2470",
pages = "391--405",
booktitle = "Malaria Immunology",
address = "United States",

}

RIS

TY - CHAP

T1 - Production of PfEMP1-specific mouse monoclonal antibodies

AU - Dalgaard, Nanna

AU - Barfod, Lea

N1 - © 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

PY - 2022

Y1 - 2022

N2 - The PfEMP1 family of proteins expressed on the Plasmodium falciparum-infected erythrocyte (IE) surface is the main target of naturally acquired immunity against malaria. Antibodies capable of opsonizing the IEs and blocking the binding between PfEMP1 and human receptors seems to be one of the main protective mechanisms of the naturally acquired immunity. Therefore this family of antigens is intensively studied. Monoclonal antibodies (mAbs) are a very valuable research tool for studying this diverse family of proteins and their interaction with human receptors. As examples, mAbs can be used to identify protective epitopes, epitopes that are targets of cross-reactive antibodies, and the surface expression of specific PfEMP1 variants. Fusing mouse splenocytes with myeloma cells to generate long-lived antibody secreting hybridoma cell lines have been used since the 1970s for the production of mAbs. In this chapter, we describe a simple, reliable, and relatively fast method for producing PfEMP1-specific mAbs from mouse spleen cells using semisolid HAT selection medium.

AB - The PfEMP1 family of proteins expressed on the Plasmodium falciparum-infected erythrocyte (IE) surface is the main target of naturally acquired immunity against malaria. Antibodies capable of opsonizing the IEs and blocking the binding between PfEMP1 and human receptors seems to be one of the main protective mechanisms of the naturally acquired immunity. Therefore this family of antigens is intensively studied. Monoclonal antibodies (mAbs) are a very valuable research tool for studying this diverse family of proteins and their interaction with human receptors. As examples, mAbs can be used to identify protective epitopes, epitopes that are targets of cross-reactive antibodies, and the surface expression of specific PfEMP1 variants. Fusing mouse splenocytes with myeloma cells to generate long-lived antibody secreting hybridoma cell lines have been used since the 1970s for the production of mAbs. In this chapter, we describe a simple, reliable, and relatively fast method for producing PfEMP1-specific mAbs from mouse spleen cells using semisolid HAT selection medium.

KW - Animals

KW - Antibodies, Monoclonal/metabolism

KW - Antibodies, Protozoan

KW - Antigens, Protozoan

KW - Epitopes/metabolism

KW - Erythrocytes/metabolism

KW - Humans

KW - Immunosuppressive Agents

KW - Malaria, Falciparum

KW - Mice

KW - Plasmodium falciparum/metabolism

KW - Protozoan Proteins/metabolism

U2 - 10.1007/978-1-0716-2189-9_29

DO - 10.1007/978-1-0716-2189-9_29

M3 - Book chapter

C2 - 35881361

SN - 978-1-0716-2188-2

VL - 2470

T3 - Methods in Molecular Biology

SP - 391

EP - 405

BT - Malaria Immunology

PB - Humana Press

ER -

ID: 323621268