Production of PfEMP1-specific human monoclonal antibodies from naturally immune individuals

Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review

Standard

Production of PfEMP1-specific human monoclonal antibodies from naturally immune individuals. / Walker, Melanie R; Barfod, Lea.

Malaria Immunology: Targeting the Surface of Infected Erythrocytes. Vol. 2470 Humana Press, 2022. p. 407-421 (Methods in molecular biology (Clifton, N.J.)).

Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review

Harvard

Walker, MR & Barfod, L 2022, Production of PfEMP1-specific human monoclonal antibodies from naturally immune individuals. in Malaria Immunology: Targeting the Surface of Infected Erythrocytes. vol. 2470, Humana Press, Methods in molecular biology (Clifton, N.J.), pp. 407-421. https://doi.org/10.1007/978-1-0716-2189-9_30

APA

Walker, M. R., & Barfod, L. (2022). Production of PfEMP1-specific human monoclonal antibodies from naturally immune individuals. In Malaria Immunology: Targeting the Surface of Infected Erythrocytes (Vol. 2470, pp. 407-421). Humana Press. Methods in molecular biology (Clifton, N.J.) https://doi.org/10.1007/978-1-0716-2189-9_30

Vancouver

Walker MR, Barfod L. Production of PfEMP1-specific human monoclonal antibodies from naturally immune individuals. In Malaria Immunology: Targeting the Surface of Infected Erythrocytes. Vol. 2470. Humana Press. 2022. p. 407-421. (Methods in molecular biology (Clifton, N.J.)). https://doi.org/10.1007/978-1-0716-2189-9_30

Author

Walker, Melanie R ; Barfod, Lea. / Production of PfEMP1-specific human monoclonal antibodies from naturally immune individuals. Malaria Immunology: Targeting the Surface of Infected Erythrocytes. Vol. 2470 Humana Press, 2022. pp. 407-421 (Methods in molecular biology (Clifton, N.J.)).

Bibtex

@inbook{6fc0f06b1e2543059f674cc83e6dafff,
title = "Production of PfEMP1-specific human monoclonal antibodies from naturally immune individuals",
abstract = "Plasmodium falciparum parasites express variable surface antigens on the infected erythrocyte surface allowing adhesion to human host receptors on the blood and endothelial cells, which can result in immune evasion. One of the most studied and key antigens in adhesion is the highly polymorphic PfEMP1. However, despite the vast variation in the PfEMP1 antigens, they are the main targets of naturally acquired immunity and are therefore promising candidates for malaria vaccine development. Generating PfEMP1-specific human monoclonal antibodies from naturally immune individuals will help to determine the best targets of protection from clinical disease. Immortalization of human B cells is one of the oldest and most efficient techniques to generate human monoclonal antibodies. Nevertheless, most protocols require flow cytometry-based cell sorting, which can be a limiting factor for many laboratories. This chapter describes an efficient protocol for the generation of PfEMP1-specific human monoclonal antibodies from malaria immune individuals that can be performed without the use of advanced cell-sorting techniques.",
keywords = "Antibodies, Monoclonal, Antibodies, Protozoan, Antigens, Protozoan, Endothelial Cells, Erythrocytes/parasitology, Humans, Malaria, Malaria, Falciparum/parasitology, Plasmodium falciparum, Protozoan Proteins",
author = "Walker, {Melanie R} and Lea Barfod",
note = "{\textcopyright} 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2022",
doi = "10.1007/978-1-0716-2189-9_30",
language = "English",
volume = "2470",
series = "Methods in molecular biology (Clifton, N.J.)",
publisher = "Humana Press",
pages = "407--421",
booktitle = "Malaria Immunology",
address = "United States",

}

RIS

TY - CHAP

T1 - Production of PfEMP1-specific human monoclonal antibodies from naturally immune individuals

AU - Walker, Melanie R

AU - Barfod, Lea

N1 - © 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

PY - 2022

Y1 - 2022

N2 - Plasmodium falciparum parasites express variable surface antigens on the infected erythrocyte surface allowing adhesion to human host receptors on the blood and endothelial cells, which can result in immune evasion. One of the most studied and key antigens in adhesion is the highly polymorphic PfEMP1. However, despite the vast variation in the PfEMP1 antigens, they are the main targets of naturally acquired immunity and are therefore promising candidates for malaria vaccine development. Generating PfEMP1-specific human monoclonal antibodies from naturally immune individuals will help to determine the best targets of protection from clinical disease. Immortalization of human B cells is one of the oldest and most efficient techniques to generate human monoclonal antibodies. Nevertheless, most protocols require flow cytometry-based cell sorting, which can be a limiting factor for many laboratories. This chapter describes an efficient protocol for the generation of PfEMP1-specific human monoclonal antibodies from malaria immune individuals that can be performed without the use of advanced cell-sorting techniques.

AB - Plasmodium falciparum parasites express variable surface antigens on the infected erythrocyte surface allowing adhesion to human host receptors on the blood and endothelial cells, which can result in immune evasion. One of the most studied and key antigens in adhesion is the highly polymorphic PfEMP1. However, despite the vast variation in the PfEMP1 antigens, they are the main targets of naturally acquired immunity and are therefore promising candidates for malaria vaccine development. Generating PfEMP1-specific human monoclonal antibodies from naturally immune individuals will help to determine the best targets of protection from clinical disease. Immortalization of human B cells is one of the oldest and most efficient techniques to generate human monoclonal antibodies. Nevertheless, most protocols require flow cytometry-based cell sorting, which can be a limiting factor for many laboratories. This chapter describes an efficient protocol for the generation of PfEMP1-specific human monoclonal antibodies from malaria immune individuals that can be performed without the use of advanced cell-sorting techniques.

KW - Antibodies, Monoclonal

KW - Antibodies, Protozoan

KW - Antigens, Protozoan

KW - Endothelial Cells

KW - Erythrocytes/parasitology

KW - Humans

KW - Malaria

KW - Malaria, Falciparum/parasitology

KW - Plasmodium falciparum

KW - Protozoan Proteins

U2 - 10.1007/978-1-0716-2189-9_30

DO - 10.1007/978-1-0716-2189-9_30

M3 - Book chapter

C2 - 35881362

VL - 2470

T3 - Methods in molecular biology (Clifton, N.J.)

SP - 407

EP - 421

BT - Malaria Immunology

PB - Humana Press

ER -

ID: 320649209