Production and purification of Plasmodium circumsporozoite protein in Lactococcus lactis

Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review

Standard

Production and purification of Plasmodium circumsporozoite protein in Lactococcus lactis. / Naghizadeh, Mohammad; Singh, Susheel K; Plieskatt, Jordan; Ofori, Ebenezer Addo; Theisen, Michael.

Recombinant Glycoproteins: Methods and Protocols. ed. / Steven B. Bradfute. Humana Press, 2024. p. 109-121 (Methods in molecular biology (Clifton, N.J.), Vol. 27521).

Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review

Harvard

Naghizadeh, M, Singh, SK, Plieskatt, J, Ofori, EA & Theisen, M 2024, Production and purification of Plasmodium circumsporozoite protein in Lactococcus lactis. in SB Bradfute (ed.), Recombinant Glycoproteins: Methods and Protocols. Humana Press, Methods in molecular biology (Clifton, N.J.), vol. 27521, pp. 109-121. https://doi.org/10.1007/978-1-0716-3666-4_7

APA

Naghizadeh, M., Singh, S. K., Plieskatt, J., Ofori, E. A., & Theisen, M. (2024). Production and purification of Plasmodium circumsporozoite protein in Lactococcus lactis. In S. B. Bradfute (Ed.), Recombinant Glycoproteins: Methods and Protocols (pp. 109-121). Humana Press. Methods in molecular biology (Clifton, N.J.) Vol. 27521 https://doi.org/10.1007/978-1-0716-3666-4_7

Vancouver

Naghizadeh M, Singh SK, Plieskatt J, Ofori EA, Theisen M. Production and purification of Plasmodium circumsporozoite protein in Lactococcus lactis. In Bradfute SB, editor, Recombinant Glycoproteins: Methods and Protocols. Humana Press. 2024. p. 109-121. (Methods in molecular biology (Clifton, N.J.), Vol. 27521). https://doi.org/10.1007/978-1-0716-3666-4_7

Author

Naghizadeh, Mohammad ; Singh, Susheel K ; Plieskatt, Jordan ; Ofori, Ebenezer Addo ; Theisen, Michael. / Production and purification of Plasmodium circumsporozoite protein in Lactococcus lactis. Recombinant Glycoproteins: Methods and Protocols. editor / Steven B. Bradfute. Humana Press, 2024. pp. 109-121 (Methods in molecular biology (Clifton, N.J.), Vol. 27521).

Bibtex

@inbook{5e562be7ae8f4b269664645925b5ba77,
title = "Production and purification of Plasmodium circumsporozoite protein in Lactococcus lactis",
abstract = "Malaria is a vector-borne disease caused by Plasmodium parasites of which Plasmodium falciparum contributed to an estimated 247 million cases worldwide in 2021 (WHO malaria report 2022). The P. falciparum Circumsporozoite protein (PfCSP) covers the surface of the sporozoite which is critical to cell invasion in the human host. PfCSP is the leading pre-erythrocytic vaccine candidate and forms the basis of the RTS'S (Mosquirix{\textregistered}) malaria vaccine. However, high-yield production of full-length PfCSP with proper folding has been challenging. Here, we describe expression and purification of full-length PfCSP (containing 4 NVDP and 38 NANP repeats) with proper conformation by a simple three-step procedure in the Lactococcus lactis expression system.",
author = "Mohammad Naghizadeh and Singh, {Susheel K} and Jordan Plieskatt and Ofori, {Ebenezer Addo} and Michael Theisen",
note = "{\textcopyright} 2024. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2024",
doi = "10.1007/978-1-0716-3666-4_7",
language = "English",
series = "Methods in molecular biology (Clifton, N.J.)",
publisher = "Humana Press",
pages = "109--121",
editor = "Bradfute, {Steven B. }",
booktitle = "Recombinant Glycoproteins",
address = "United States",

}

RIS

TY - CHAP

T1 - Production and purification of Plasmodium circumsporozoite protein in Lactococcus lactis

AU - Naghizadeh, Mohammad

AU - Singh, Susheel K

AU - Plieskatt, Jordan

AU - Ofori, Ebenezer Addo

AU - Theisen, Michael

N1 - © 2024. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

PY - 2024

Y1 - 2024

N2 - Malaria is a vector-borne disease caused by Plasmodium parasites of which Plasmodium falciparum contributed to an estimated 247 million cases worldwide in 2021 (WHO malaria report 2022). The P. falciparum Circumsporozoite protein (PfCSP) covers the surface of the sporozoite which is critical to cell invasion in the human host. PfCSP is the leading pre-erythrocytic vaccine candidate and forms the basis of the RTS'S (Mosquirix®) malaria vaccine. However, high-yield production of full-length PfCSP with proper folding has been challenging. Here, we describe expression and purification of full-length PfCSP (containing 4 NVDP and 38 NANP repeats) with proper conformation by a simple three-step procedure in the Lactococcus lactis expression system.

AB - Malaria is a vector-borne disease caused by Plasmodium parasites of which Plasmodium falciparum contributed to an estimated 247 million cases worldwide in 2021 (WHO malaria report 2022). The P. falciparum Circumsporozoite protein (PfCSP) covers the surface of the sporozoite which is critical to cell invasion in the human host. PfCSP is the leading pre-erythrocytic vaccine candidate and forms the basis of the RTS'S (Mosquirix®) malaria vaccine. However, high-yield production of full-length PfCSP with proper folding has been challenging. Here, we describe expression and purification of full-length PfCSP (containing 4 NVDP and 38 NANP repeats) with proper conformation by a simple three-step procedure in the Lactococcus lactis expression system.

U2 - 10.1007/978-1-0716-3666-4_7

DO - 10.1007/978-1-0716-3666-4_7

M3 - Book chapter

C2 - 38315362

T3 - Methods in molecular biology (Clifton, N.J.)

SP - 109

EP - 121

BT - Recombinant Glycoproteins

A2 - Bradfute, Steven B.

PB - Humana Press

ER -

ID: 382902656