Plasmodium falciparum transcriptome analysis reveals pregnancy malaria associated gene expression
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Plasmodium falciparum transcriptome analysis reveals pregnancy malaria associated gene expression. / Tuikue Ndam, Nicaise; Bischoff, Emmanuel; Proux, Caroline; Lavstsen, Thomas; Salanti, Ali; Guitard, Juliette; Nielsen, Morten A; Coppée, Jean-Yves; Gaye, Alioune; Theander, Thor; David, Peter H; Deloron, Philippe.
In: PLoS ONE, Vol. 3, No. 3, 2008, p. e1855.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Plasmodium falciparum transcriptome analysis reveals pregnancy malaria associated gene expression
AU - Tuikue Ndam, Nicaise
AU - Bischoff, Emmanuel
AU - Proux, Caroline
AU - Lavstsen, Thomas
AU - Salanti, Ali
AU - Guitard, Juliette
AU - Nielsen, Morten A
AU - Coppée, Jean-Yves
AU - Gaye, Alioune
AU - Theander, Thor
AU - David, Peter H
AU - Deloron, Philippe
N1 - Keywords: Animals; Female; Humans; Malaria, Falciparum; Placenta; Plasmodium falciparum; Pregnancy; Pregnancy Complications, Parasitic; RNA, Messenger
PY - 2008
Y1 - 2008
N2 - BACKGROUND: Pregnancy-associated malaria (PAM) causing maternal anemia and low birth weight is among the multiple manifestations of Plasmodium falciparum malaria. Infected erythrocytes (iEs) can acquire various adhesive properties that mediate the clinical severity of malaria. Recent advances on the molecular basis of virulence and immune evasion have helped identify var2csa as a PAM-specific var gene. METHODOLOGY/PRINCIPAL FINDINGS: The present study presents a genome-wide microarray transcript analysis of 18 P. falciparum parasite isolates freshly collected from the placenta. The proportion of PAM over-expressed genes located in subtelomeric regions as well as that of PAM over-expressed genes predicted to be exported were higher than expected compared to the whole genome. The identification of novel parasite molecules with specificity to PAM and which are likely involved in host-pathogen interactions and placental tropism is described. One of these proteins, PFI1785w, was further characterized as the product of a two-exon PHIST gene, and was more often recognized by serum samples from P. falciparum-exposed women than from men. CONCLUSIONS/SIGNIFICANCE: These findings suggest that other parasite proteins, such as PFI1785w, may contribute beside VAR2CSA to the pathogenesis of PAM. These data may be very valuable for future vaccine development.
AB - BACKGROUND: Pregnancy-associated malaria (PAM) causing maternal anemia and low birth weight is among the multiple manifestations of Plasmodium falciparum malaria. Infected erythrocytes (iEs) can acquire various adhesive properties that mediate the clinical severity of malaria. Recent advances on the molecular basis of virulence and immune evasion have helped identify var2csa as a PAM-specific var gene. METHODOLOGY/PRINCIPAL FINDINGS: The present study presents a genome-wide microarray transcript analysis of 18 P. falciparum parasite isolates freshly collected from the placenta. The proportion of PAM over-expressed genes located in subtelomeric regions as well as that of PAM over-expressed genes predicted to be exported were higher than expected compared to the whole genome. The identification of novel parasite molecules with specificity to PAM and which are likely involved in host-pathogen interactions and placental tropism is described. One of these proteins, PFI1785w, was further characterized as the product of a two-exon PHIST gene, and was more often recognized by serum samples from P. falciparum-exposed women than from men. CONCLUSIONS/SIGNIFICANCE: These findings suggest that other parasite proteins, such as PFI1785w, may contribute beside VAR2CSA to the pathogenesis of PAM. These data may be very valuable for future vaccine development.
U2 - 10.1371/journal.pone.0001855
DO - 10.1371/journal.pone.0001855
M3 - Journal article
C2 - 18365010
VL - 3
SP - e1855
JO - PLoS ONE
JF - PLoS ONE
SN - 1932-6203
IS - 3
ER -
ID: 6765015