Plasmodium berghei ANKA: erythropoietin activates neural stem cells in an experimental cerebral malaria model
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Plasmodium berghei ANKA: erythropoietin activates neural stem cells in an experimental cerebral malaria model. / Core, Andrew; Hempel, Casper; Kurtzhals, Jørgen A L; Penkowa, Milena.
In: Experimental Parasitology, Vol. 127, No. 2, 2011, p. 500-5.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Plasmodium berghei ANKA: erythropoietin activates neural stem cells in an experimental cerebral malaria model
AU - Core, Andrew
AU - Hempel, Casper
AU - Kurtzhals, Jørgen A L
AU - Penkowa, Milena
N1 - Copyright © 2010 Elsevier Inc. All rights reserved.
PY - 2011
Y1 - 2011
N2 - Cerebral malaria (CM) causes substantial mortality and neurological sequelae in survivors, and no neuroprotective regimens are currently available for this condition. Erythropoietin (EPO) reduces neuropathology and improves survival in murine CM. Using the Plasmodium berghei model of CM, we investigated if EPO's neuroprotective effects include activation of endogenous neural stem cells (NSC). By using immunohistochemical markers of different NSC maturation stages, we show that EPO increased the number of nestin(+) cells in the dentate gyrus and in the sub-ventricular zone of the lateral ventricles, relative to control-treatment. 75% of the EPO-treated CM mice displayed migration as nestin(+) NSC. The NSC showed differentiation towards a neural cell lineage as shown by PSA-NCAM binding and NSC maturation and lineage commitment was significantly affected by exogenous EPO and by CM in the sub ventricular zone. These results indicate a rapid, EPO-dependent activation of NSC during CM pathology.
AB - Cerebral malaria (CM) causes substantial mortality and neurological sequelae in survivors, and no neuroprotective regimens are currently available for this condition. Erythropoietin (EPO) reduces neuropathology and improves survival in murine CM. Using the Plasmodium berghei model of CM, we investigated if EPO's neuroprotective effects include activation of endogenous neural stem cells (NSC). By using immunohistochemical markers of different NSC maturation stages, we show that EPO increased the number of nestin(+) cells in the dentate gyrus and in the sub-ventricular zone of the lateral ventricles, relative to control-treatment. 75% of the EPO-treated CM mice displayed migration as nestin(+) NSC. The NSC showed differentiation towards a neural cell lineage as shown by PSA-NCAM binding and NSC maturation and lineage commitment was significantly affected by exogenous EPO and by CM in the sub ventricular zone. These results indicate a rapid, EPO-dependent activation of NSC during CM pathology.
KW - Analysis of Variance
KW - Animals
KW - Disease Models, Animal
KW - Erythropoietin
KW - Female
KW - Immunohistochemistry
KW - Intermediate Filament Proteins
KW - Malaria, Cerebral
KW - Mice
KW - Mice, Inbred C57BL
KW - Nerve Tissue Proteins
KW - Neural Cell Adhesion Molecule L1
KW - Neural Stem Cells
KW - Neurites
KW - Neuroprotective Agents
KW - Plasmodium berghei
KW - Sialic Acids
KW - Specific Pathogen-Free Organisms
U2 - 10.1016/j.exppara.2010.09.010
DO - 10.1016/j.exppara.2010.09.010
M3 - Journal article
C2 - 21044627
VL - 127
SP - 500
EP - 505
JO - Experimental Parasitology
JF - Experimental Parasitology
SN - 0014-4894
IS - 2
ER -
ID: 32646328