Optimisation and standardisation of a multiplex immunoassay of diverse plasmodium falciparum antigens to assess changes in malaria transmission using sero-epidemiology

Research output: Contribution to journalJournal articleResearchpeer-review

  • Lindsey Wu
  • Tom Hall
  • Isaac Ssewanyana
  • Tate Oulton
  • Catriona Patterson
  • Hristina Vasileva
  • Susheel Singh
  • Muna Affara
  • Julia Mwesigwa
  • Simon Correa
  • Mamadou Bah
  • Umberto D'alessandro
  • Nuno Sepúlveda
  • Chris Drakeley
  • Kevin K.A. Tetteh

Background: Antibody responses have been used to characterise transmission and exposure history in malaria-endemic settings for over a decade. Such studies have typically been conducted on well-standardised enzyme-linked immunosorbent assays (ELISAs). However, recently developed quantitative suspension array technologies (qSAT) are now capable of high-throughput and multiplexed screening of up to hundreds of analytes at a time. This study presents a customised protocol for the Luminex MAGPIX

Original languageEnglish
Article number26
JournalWellcome Open Research
Volume4
ISSN2398-502X
DOIs
Publication statusPublished - 2019

    Research areas

  • Antibodies, Luminex, MAGPIX, Malaria, Plasmodium, Serology, Serum

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