New filtration system for efficient recovery of waterborne Cryptosporidium oocysts and Giardia cysts

Research output: Contribution to journal › Journal article › Research › peer-review

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New filtration system for efficient recovery of waterborne Cryptosporidium oocysts and Giardia cysts. / Al-Sabi, Mohammad Nafi Solaiman; Gad, J A; Riber, U; Kurtzhals, J A L; Enemark, H. L.

In: Journal of Applied Microbiology, Vol. 119, No. 3, 09.2015, p. 894-903.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Al-Sabi, MNS, Gad, JA, Riber, U, Kurtzhals, JAL & Enemark, HL 2015, 'New filtration system for efficient recovery of waterborne Cryptosporidium oocysts and Giardia cysts', Journal of Applied Microbiology, vol. 119, no. 3, pp. 894-903. https://doi.org/10.1111/jam.12898

APA

Al-Sabi, M. N. S., Gad, J. A., Riber, U., Kurtzhals, J. A. L., & Enemark, H. L. (2015). New filtration system for efficient recovery of waterborne Cryptosporidium oocysts and Giardia cysts. Journal of Applied Microbiology, 119(3), 894-903. https://doi.org/10.1111/jam.12898

Vancouver

Al-Sabi MNS, Gad JA, Riber U, Kurtzhals JAL, Enemark HL. New filtration system for efficient recovery of waterborne Cryptosporidium oocysts and Giardia cysts. Journal of Applied Microbiology. 2015 Sep;119(3):894-903. https://doi.org/10.1111/jam.12898

Author

Al-Sabi, Mohammad Nafi Solaiman ; Gad, J A ; Riber, U ; Kurtzhals, J A L ; Enemark, H. L. / New filtration system for efficient recovery of waterborne Cryptosporidium oocysts and Giardia cysts. In: Journal of Applied Microbiology. 2015 ; Vol. 119, No. 3. pp. 894-903.

Bibtex

@article{ad0580370bcc4353acc690d01aa8bf2f,
title = "New filtration system for efficient recovery of waterborne Cryptosporidium oocysts and Giardia cysts",
abstract = "AIMS: To develop a filtration unit for efficient recovery of waterborne Cryptosporidium oocysts and Giardia cysts ((oo-)cysts) in drinking water.METHODS AND RESULTS: This unit utilizes a metallic filter and an ultrasound transducer for eluting (oo-)cysts, with a fixed retentate backwash volume; approx. 400 μl. Changes in the viability was evaluated by seeding wild type (oo-)cysts (1 × 10(4) ) followed by sonication for 5, 10, 20 or 40 s (five replicates for each period). Flow cytometry analysis showed negligible increase in the mortality of (oo-)cysts exposed to 5-10 s of sonication. Recovery rate was assessed by seeding ColorSeed({\texttrademark}) (10 replicates) into the filter unit followed by air backwash to a glass slide and counting of (oo-)cysts by epifluorescent microscopy. High recovery rates (mean ± SD) were found: 84·9% ± 4·8 for Giardia cysts and 70% ± 6·5 for Cryptosporidium oocysts. DNA of seeded wild type (oo-)cysts (1 × 10(2) ; 10 replicates) was successfully amplified using real-time PCR.CONCLUSIONS: The use of a metallic filter, sonication and 'air backwash' were key factors for creating a highly efficient system for recovery of apparently undamaged protozoa.SIGNIFICANCE AND IMPACT OF THE STUDY: This reagent-less system can be used for monitoring of parasite contamination in drinking water.",
author = "Al-Sabi, {Mohammad Nafi Solaiman} and Gad, {J A} and U Riber and Kurtzhals, {J A L} and Enemark, {H. L.}",
note = "{\textcopyright} 2015 The Society for Applied Microbiology.",
year = "2015",
month = sep,
doi = "10.1111/jam.12898",
language = "English",
volume = "119",
pages = "894--903",
journal = "Proceedings of the Society for Applied Bacteriology",
issn = "0370-1778",
publisher = "Wiley-Blackwell",
number = "3",

}

RIS

TY - JOUR

T1 - New filtration system for efficient recovery of waterborne Cryptosporidium oocysts and Giardia cysts

AU - Al-Sabi, Mohammad Nafi Solaiman

AU - Gad, J A

AU - Riber, U

AU - Kurtzhals, J A L

AU - Enemark, H. L.

N1 - © 2015 The Society for Applied Microbiology.

PY - 2015/9

Y1 - 2015/9

N2 - AIMS: To develop a filtration unit for efficient recovery of waterborne Cryptosporidium oocysts and Giardia cysts ((oo-)cysts) in drinking water.METHODS AND RESULTS: This unit utilizes a metallic filter and an ultrasound transducer for eluting (oo-)cysts, with a fixed retentate backwash volume; approx. 400 μl. Changes in the viability was evaluated by seeding wild type (oo-)cysts (1 × 10(4) ) followed by sonication for 5, 10, 20 or 40 s (five replicates for each period). Flow cytometry analysis showed negligible increase in the mortality of (oo-)cysts exposed to 5-10 s of sonication. Recovery rate was assessed by seeding ColorSeed(™) (10 replicates) into the filter unit followed by air backwash to a glass slide and counting of (oo-)cysts by epifluorescent microscopy. High recovery rates (mean ± SD) were found: 84·9% ± 4·8 for Giardia cysts and 70% ± 6·5 for Cryptosporidium oocysts. DNA of seeded wild type (oo-)cysts (1 × 10(2) ; 10 replicates) was successfully amplified using real-time PCR.CONCLUSIONS: The use of a metallic filter, sonication and 'air backwash' were key factors for creating a highly efficient system for recovery of apparently undamaged protozoa.SIGNIFICANCE AND IMPACT OF THE STUDY: This reagent-less system can be used for monitoring of parasite contamination in drinking water.

AB - AIMS: To develop a filtration unit for efficient recovery of waterborne Cryptosporidium oocysts and Giardia cysts ((oo-)cysts) in drinking water.METHODS AND RESULTS: This unit utilizes a metallic filter and an ultrasound transducer for eluting (oo-)cysts, with a fixed retentate backwash volume; approx. 400 μl. Changes in the viability was evaluated by seeding wild type (oo-)cysts (1 × 10(4) ) followed by sonication for 5, 10, 20 or 40 s (five replicates for each period). Flow cytometry analysis showed negligible increase in the mortality of (oo-)cysts exposed to 5-10 s of sonication. Recovery rate was assessed by seeding ColorSeed(™) (10 replicates) into the filter unit followed by air backwash to a glass slide and counting of (oo-)cysts by epifluorescent microscopy. High recovery rates (mean ± SD) were found: 84·9% ± 4·8 for Giardia cysts and 70% ± 6·5 for Cryptosporidium oocysts. DNA of seeded wild type (oo-)cysts (1 × 10(2) ; 10 replicates) was successfully amplified using real-time PCR.CONCLUSIONS: The use of a metallic filter, sonication and 'air backwash' were key factors for creating a highly efficient system for recovery of apparently undamaged protozoa.SIGNIFICANCE AND IMPACT OF THE STUDY: This reagent-less system can be used for monitoring of parasite contamination in drinking water.

U2 - 10.1111/jam.12898

DO - 10.1111/jam.12898

M3 - Journal article

C2 - 26172033

VL - 119

SP - 894

EP - 903

JO - Proceedings of the Society for Applied Bacteriology

JF - Proceedings of the Society for Applied Bacteriology

SN - 0370-1778

IS - 3

ER -

ID: 143165040