Insect cells are superior to Escherichia coli in producing malaria proteins inducing IgG targeting PfEMP1 on infected erythrocytes

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Standard

Insect cells are superior to Escherichia coli in producing malaria proteins inducing IgG targeting PfEMP1 on infected erythrocytes. / Victor, Michala E; Bengtsson, Anja; Andersen, Gorm; Bengtsson, Dominique; Lusingu, John P; Vestergaard, Lasse S; Arnot, David E; Theander, Thor G; Joergensen, Louise; Jensen, Anja T R.

In: Malaria Journal, Vol. 9, 2010, p. 325.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Victor, ME, Bengtsson, A, Andersen, G, Bengtsson, D, Lusingu, JP, Vestergaard, LS, Arnot, DE, Theander, TG, Joergensen, L & Jensen, ATR 2010, 'Insect cells are superior to Escherichia coli in producing malaria proteins inducing IgG targeting PfEMP1 on infected erythrocytes', Malaria Journal, vol. 9, pp. 325. https://doi.org/10.1186/1475-2875-9-325

APA

Victor, M. E., Bengtsson, A., Andersen, G., Bengtsson, D., Lusingu, J. P., Vestergaard, L. S., Arnot, D. E., Theander, T. G., Joergensen, L., & Jensen, A. T. R. (2010). Insect cells are superior to Escherichia coli in producing malaria proteins inducing IgG targeting PfEMP1 on infected erythrocytes. Malaria Journal, 9, 325. https://doi.org/10.1186/1475-2875-9-325

Vancouver

Victor ME, Bengtsson A, Andersen G, Bengtsson D, Lusingu JP, Vestergaard LS et al. Insect cells are superior to Escherichia coli in producing malaria proteins inducing IgG targeting PfEMP1 on infected erythrocytes. Malaria Journal. 2010;9:325. https://doi.org/10.1186/1475-2875-9-325

Author

Victor, Michala E ; Bengtsson, Anja ; Andersen, Gorm ; Bengtsson, Dominique ; Lusingu, John P ; Vestergaard, Lasse S ; Arnot, David E ; Theander, Thor G ; Joergensen, Louise ; Jensen, Anja T R. / Insect cells are superior to Escherichia coli in producing malaria proteins inducing IgG targeting PfEMP1 on infected erythrocytes. In: Malaria Journal. 2010 ; Vol. 9. pp. 325.

Bibtex

@article{0043d73774dc4d4cab56e6082cb5f4ff,
title = "Insect cells are superior to Escherichia coli in producing malaria proteins inducing IgG targeting PfEMP1 on infected erythrocytes",
abstract = "The PFD1235w Plasmodium falciparum erythrocyte membrane protein 1 (PfEMP1) antigen is associated with severe malaria in children and can be expressed on the surface of infected erythrocytes (IE) adhering to ICAM1. However, the exact three-dimensional structure of this PfEMP1 and its surface-exposed epitopes are unknown. An insect cell and Escherichia coli based system was used to express single and double domains encoded by the pfd1235w var gene. The resulting recombinant proteins have been evaluated for yield and purity and their ability to induce rat antibodies, which react with the native PFD1235w PfEMP1 antigen expressed on 3D7PFD1235w-IE. Their recognition by human anti-malaria antibodies from previously infected Tanzanian donors was also analysed.",
keywords = "Adolescent, Animals, Antibodies, Protozoan, Baculoviridae, Cell Line, Child, Child, Preschool, Escherichia coli, Humans, Immunoglobulin G, Insects, Malaria Vaccines, Malaria, Falciparum, Protozoan Proteins, Rats, Tanzania, Vaccines, Synthetic, Young Adult",
author = "Victor, {Michala E} and Anja Bengtsson and Gorm Andersen and Dominique Bengtsson and Lusingu, {John P} and Vestergaard, {Lasse S} and Arnot, {David E} and Theander, {Thor G} and Louise Joergensen and Jensen, {Anja T R}",
year = "2010",
doi = "10.1186/1475-2875-9-325",
language = "English",
volume = "9",
pages = "325",
journal = "Malaria Journal",
issn = "1475-2875",
publisher = "BioMed Central",

}

RIS

TY - JOUR

T1 - Insect cells are superior to Escherichia coli in producing malaria proteins inducing IgG targeting PfEMP1 on infected erythrocytes

AU - Victor, Michala E

AU - Bengtsson, Anja

AU - Andersen, Gorm

AU - Bengtsson, Dominique

AU - Lusingu, John P

AU - Vestergaard, Lasse S

AU - Arnot, David E

AU - Theander, Thor G

AU - Joergensen, Louise

AU - Jensen, Anja T R

PY - 2010

Y1 - 2010

N2 - The PFD1235w Plasmodium falciparum erythrocyte membrane protein 1 (PfEMP1) antigen is associated with severe malaria in children and can be expressed on the surface of infected erythrocytes (IE) adhering to ICAM1. However, the exact three-dimensional structure of this PfEMP1 and its surface-exposed epitopes are unknown. An insect cell and Escherichia coli based system was used to express single and double domains encoded by the pfd1235w var gene. The resulting recombinant proteins have been evaluated for yield and purity and their ability to induce rat antibodies, which react with the native PFD1235w PfEMP1 antigen expressed on 3D7PFD1235w-IE. Their recognition by human anti-malaria antibodies from previously infected Tanzanian donors was also analysed.

AB - The PFD1235w Plasmodium falciparum erythrocyte membrane protein 1 (PfEMP1) antigen is associated with severe malaria in children and can be expressed on the surface of infected erythrocytes (IE) adhering to ICAM1. However, the exact three-dimensional structure of this PfEMP1 and its surface-exposed epitopes are unknown. An insect cell and Escherichia coli based system was used to express single and double domains encoded by the pfd1235w var gene. The resulting recombinant proteins have been evaluated for yield and purity and their ability to induce rat antibodies, which react with the native PFD1235w PfEMP1 antigen expressed on 3D7PFD1235w-IE. Their recognition by human anti-malaria antibodies from previously infected Tanzanian donors was also analysed.

KW - Adolescent

KW - Animals

KW - Antibodies, Protozoan

KW - Baculoviridae

KW - Cell Line

KW - Child

KW - Child, Preschool

KW - Escherichia coli

KW - Humans

KW - Immunoglobulin G

KW - Insects

KW - Malaria Vaccines

KW - Malaria, Falciparum

KW - Protozoan Proteins

KW - Rats

KW - Tanzania

KW - Vaccines, Synthetic

KW - Young Adult

U2 - 10.1186/1475-2875-9-325

DO - 10.1186/1475-2875-9-325

M3 - Journal article

C2 - 21078147

VL - 9

SP - 325

JO - Malaria Journal

JF - Malaria Journal

SN - 1475-2875

ER -

ID: 33149150