Immunomagnetic Selection of Plasmodium falciparum–Infected Erythrocytes Expressing Particular PfEMP1 Variants
Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review
Plasmodium falciparum expresses a broad range of proteins on the surface of infected erythrocytes (IEs), including members of the P. falciparum erythrocyte membrane protein 1 (PfEMP1) family. This protocol describes an immunomagnetic selection method using PfEMP1-specific antibodies to obtain a parasite clone homogenously expressing a particular PfEMP1 protein. The expression of the corresponding PfEMP1 is later tested by flow cytometry, and the selected parasites can be used for further analysis.
| Original language | English |
|---|---|
| Title of host publication | Methods in Molecular Biology : Targeting the Surface of Infected Erythrocytes |
| Number of pages | 10 |
| Publisher | Humana Press |
| Publication date | 2022 |
| Pages | 69-78 |
| ISBN (Electronic) | 978-1-0716-2189-9 |
| DOIs | |
| Publication status | Published - 2022 |
| Series | Methods in Molecular Biology |
|---|---|
| Volume | 2470 |
| ISSN | 1064-3745 |
Bibliographical note
Funding Information:
The authors’ research work is supported by the Danish International Development Agency—Danida (17-02-KU). Maiken Visti and Mette Madsen are thanked for their technical assistance. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Publisher Copyright:
© 2022, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.
- Antibodies, Antiserum, Flow cytometry, Immunomagnetic selection, Magnetic beads, PfEMP1
Research areas
ID: 320006441