IgG responses to the Plasmodium falciparum antigen VAR2CSA in Colombia are restricted to pregnancy and are not induced by exposure to Plasmodium vivax

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Standard

IgG responses to the Plasmodium falciparum antigen VAR2CSA in Colombia are restricted to pregnancy and are not induced by exposure to Plasmodium vivax. / Lopez-Perez, Mary; Larsen, Mads Delbo; Bayarri-Olmos, Rafael; Ampomah, Paulina; Stevenson, Liz; Arévalo-Herrera, Myriam; Herrera, Sócrates; Hviid, Lars.

In: Infection and Immunity, Vol. 86, No. 8, e00136-18, 2018.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Lopez-Perez, M, Larsen, MD, Bayarri-Olmos, R, Ampomah, P, Stevenson, L, Arévalo-Herrera, M, Herrera, S & Hviid, L 2018, 'IgG responses to the Plasmodium falciparum antigen VAR2CSA in Colombia are restricted to pregnancy and are not induced by exposure to Plasmodium vivax', Infection and Immunity, vol. 86, no. 8, e00136-18. https://doi.org/10.1128/IAI.00136-18

APA

Lopez-Perez, M., Larsen, M. D., Bayarri-Olmos, R., Ampomah, P., Stevenson, L., Arévalo-Herrera, M., Herrera, S., & Hviid, L. (2018). IgG responses to the Plasmodium falciparum antigen VAR2CSA in Colombia are restricted to pregnancy and are not induced by exposure to Plasmodium vivax. Infection and Immunity, 86(8), [e00136-18]. https://doi.org/10.1128/IAI.00136-18

Vancouver

Lopez-Perez M, Larsen MD, Bayarri-Olmos R, Ampomah P, Stevenson L, Arévalo-Herrera M et al. IgG responses to the Plasmodium falciparum antigen VAR2CSA in Colombia are restricted to pregnancy and are not induced by exposure to Plasmodium vivax. Infection and Immunity. 2018;86(8). e00136-18. https://doi.org/10.1128/IAI.00136-18

Author

Lopez-Perez, Mary ; Larsen, Mads Delbo ; Bayarri-Olmos, Rafael ; Ampomah, Paulina ; Stevenson, Liz ; Arévalo-Herrera, Myriam ; Herrera, Sócrates ; Hviid, Lars. / IgG responses to the Plasmodium falciparum antigen VAR2CSA in Colombia are restricted to pregnancy and are not induced by exposure to Plasmodium vivax. In: Infection and Immunity. 2018 ; Vol. 86, No. 8.

Bibtex

@article{9800f9c8aa7146478bafea67454ecf2d,
title = "IgG responses to the Plasmodium falciparum antigen VAR2CSA in Colombia are restricted to pregnancy and are not induced by exposure to Plasmodium vivax",
abstract = "Clinical immunity to malaria is associated with acquisition of IgG specific for members of the Plasmodium falciparum erythrocyte membrane protein 1 (PfEMP1) family of clonally variant antigens on the surface of infected erythrocytes (IEs). The VAR2CSA subtype of PfEMP1 mediates IE binding in the placenta. VAR2CSA-specific IgG is normally acquired only after exposure to placental parasites. However, it was recently reported that men and children from Colombia often have high levels of functional VAR2CSA-specific IgG. This potentially undermines the current understanding of malaria immunity in pregnant women, and we thus conducted a study to assess further the levels of VAR2CSA-specific IgG in pregnant and non-pregnant Colombians.We found that plasma IgG against two full-length recombinant PfEMP1 proteins (one VAR2CSA-type and one not) produced in baculovirus-transfected insect cells was frequent among Colombian men, children, and pregnant women with acute or previous malaria exposure. In contrast, IgG reactivity to a homologous full-length VAR2CSA-type protein expressed in Chinese hamster ovary (CHO) cells was low and infrequent among the Colombian plasma samples, as was reactivity to both corresponding native PfEMP1 proteins. Moreover, human and rabbit antibodies specific for P. vivax Duffy-binding protein (PvDBP), a protein with some homology to PfEMP1, did not react with VAR2CSA-type recombinant or native proteins, although the mouse monoclonal and PvDBP-specific antibody 3D10 was weakly reactive with recombinant proteins expressed in baculovirus-transfected insect cells.Our data indicate that the previously reported Colombian IgG reactivity to recombinant VAR2CSA is not malaria-specific, and that acquisition of VAR2CSA-specific IgG is restricted to pregnancy, in Colombia and elsewhere.",
author = "Mary Lopez-Perez and Larsen, {Mads Delbo} and Rafael Bayarri-Olmos and Paulina Ampomah and Liz Stevenson and Myriam Ar{\'e}valo-Herrera and S{\'o}crates Herrera and Lars Hviid",
note = "Copyright {\textcopyright} 2018 Lopez-Perez et al.",
year = "2018",
doi = "10.1128/IAI.00136-18",
language = "English",
volume = "86",
journal = "Infection and Immunity",
issn = "0019-9567",
publisher = "American Society for Microbiology",
number = "8",

}

RIS

TY - JOUR

T1 - IgG responses to the Plasmodium falciparum antigen VAR2CSA in Colombia are restricted to pregnancy and are not induced by exposure to Plasmodium vivax

AU - Lopez-Perez, Mary

AU - Larsen, Mads Delbo

AU - Bayarri-Olmos, Rafael

AU - Ampomah, Paulina

AU - Stevenson, Liz

AU - Arévalo-Herrera, Myriam

AU - Herrera, Sócrates

AU - Hviid, Lars

N1 - Copyright © 2018 Lopez-Perez et al.

PY - 2018

Y1 - 2018

N2 - Clinical immunity to malaria is associated with acquisition of IgG specific for members of the Plasmodium falciparum erythrocyte membrane protein 1 (PfEMP1) family of clonally variant antigens on the surface of infected erythrocytes (IEs). The VAR2CSA subtype of PfEMP1 mediates IE binding in the placenta. VAR2CSA-specific IgG is normally acquired only after exposure to placental parasites. However, it was recently reported that men and children from Colombia often have high levels of functional VAR2CSA-specific IgG. This potentially undermines the current understanding of malaria immunity in pregnant women, and we thus conducted a study to assess further the levels of VAR2CSA-specific IgG in pregnant and non-pregnant Colombians.We found that plasma IgG against two full-length recombinant PfEMP1 proteins (one VAR2CSA-type and one not) produced in baculovirus-transfected insect cells was frequent among Colombian men, children, and pregnant women with acute or previous malaria exposure. In contrast, IgG reactivity to a homologous full-length VAR2CSA-type protein expressed in Chinese hamster ovary (CHO) cells was low and infrequent among the Colombian plasma samples, as was reactivity to both corresponding native PfEMP1 proteins. Moreover, human and rabbit antibodies specific for P. vivax Duffy-binding protein (PvDBP), a protein with some homology to PfEMP1, did not react with VAR2CSA-type recombinant or native proteins, although the mouse monoclonal and PvDBP-specific antibody 3D10 was weakly reactive with recombinant proteins expressed in baculovirus-transfected insect cells.Our data indicate that the previously reported Colombian IgG reactivity to recombinant VAR2CSA is not malaria-specific, and that acquisition of VAR2CSA-specific IgG is restricted to pregnancy, in Colombia and elsewhere.

AB - Clinical immunity to malaria is associated with acquisition of IgG specific for members of the Plasmodium falciparum erythrocyte membrane protein 1 (PfEMP1) family of clonally variant antigens on the surface of infected erythrocytes (IEs). The VAR2CSA subtype of PfEMP1 mediates IE binding in the placenta. VAR2CSA-specific IgG is normally acquired only after exposure to placental parasites. However, it was recently reported that men and children from Colombia often have high levels of functional VAR2CSA-specific IgG. This potentially undermines the current understanding of malaria immunity in pregnant women, and we thus conducted a study to assess further the levels of VAR2CSA-specific IgG in pregnant and non-pregnant Colombians.We found that plasma IgG against two full-length recombinant PfEMP1 proteins (one VAR2CSA-type and one not) produced in baculovirus-transfected insect cells was frequent among Colombian men, children, and pregnant women with acute or previous malaria exposure. In contrast, IgG reactivity to a homologous full-length VAR2CSA-type protein expressed in Chinese hamster ovary (CHO) cells was low and infrequent among the Colombian plasma samples, as was reactivity to both corresponding native PfEMP1 proteins. Moreover, human and rabbit antibodies specific for P. vivax Duffy-binding protein (PvDBP), a protein with some homology to PfEMP1, did not react with VAR2CSA-type recombinant or native proteins, although the mouse monoclonal and PvDBP-specific antibody 3D10 was weakly reactive with recombinant proteins expressed in baculovirus-transfected insect cells.Our data indicate that the previously reported Colombian IgG reactivity to recombinant VAR2CSA is not malaria-specific, and that acquisition of VAR2CSA-specific IgG is restricted to pregnancy, in Colombia and elsewhere.

U2 - 10.1128/IAI.00136-18

DO - 10.1128/IAI.00136-18

M3 - Journal article

C2 - 29784859

VL - 86

JO - Infection and Immunity

JF - Infection and Immunity

SN - 0019-9567

IS - 8

M1 - e00136-18

ER -

ID: 199883598