Identification of a major rif transcript common to gametocytes and sporozoites of Plasmodium falciparum
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Identification of a major rif transcript common to gametocytes and sporozoites of Plasmodium falciparum. / Wang, Christian W; Mwakalinga, Steven B; Sutherland, Colin J; Schwank, Samana; Sharp, Sarah; Hermsen, Cornelus C; Sauerwein, Robert W; Theander, Thor G; Lavstsen, Thomas.
In: Malaria Journal, Vol. 9, No. 1, 2010, p. 147.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Identification of a major rif transcript common to gametocytes and sporozoites of Plasmodium falciparum
AU - Wang, Christian W
AU - Mwakalinga, Steven B
AU - Sutherland, Colin J
AU - Schwank, Samana
AU - Sharp, Sarah
AU - Hermsen, Cornelus C
AU - Sauerwein, Robert W
AU - Theander, Thor G
AU - Lavstsen, Thomas
PY - 2010
Y1 - 2010
N2 - ABSTRACT: BACKGROUND: The Plasmodium falciparum parasite is transmitted in its sexual gametocyte stage from man to mosquito and as asexual sporozoites from mosquito to man. Developing gametocytes sequester preferentially in the bone marrow, but mature stage gametocytes are released to the bloodstream. Sexual stage parasite surface proteins are of interest as candidate target antigens for transmission blocking vaccines. METHODS: In this study, the transcript profiles of rif and var genes, known to encode surface antigens in asexual blood stage parasites, were investigated at different stages of 3D7/NF54 gametocytogenesis and in sporozoites. RESULTS: Gametocytes exhibited a rif transcript profile unlinked to the rif and var transcript profile of the asexual progenitors. At stage V, mature gametocytes produced high levels of a single rif gene, PF13_0006, which also dominated the rif transcript profile of sporozoites. All var genes appeared to be silenced in sporozoites. CONCLUSIONS: The most prominent variant surface antigen transcribed in both gametocytes and sporozoites of 3D7/NF54 is a single variant of the RIFIN protein family. This discovery may lead to the identification of the parasites binding ligands responsible for the adhesion during sexual stages and potentially to novel vaccine candidates.
AB - ABSTRACT: BACKGROUND: The Plasmodium falciparum parasite is transmitted in its sexual gametocyte stage from man to mosquito and as asexual sporozoites from mosquito to man. Developing gametocytes sequester preferentially in the bone marrow, but mature stage gametocytes are released to the bloodstream. Sexual stage parasite surface proteins are of interest as candidate target antigens for transmission blocking vaccines. METHODS: In this study, the transcript profiles of rif and var genes, known to encode surface antigens in asexual blood stage parasites, were investigated at different stages of 3D7/NF54 gametocytogenesis and in sporozoites. RESULTS: Gametocytes exhibited a rif transcript profile unlinked to the rif and var transcript profile of the asexual progenitors. At stage V, mature gametocytes produced high levels of a single rif gene, PF13_0006, which also dominated the rif transcript profile of sporozoites. All var genes appeared to be silenced in sporozoites. CONCLUSIONS: The most prominent variant surface antigen transcribed in both gametocytes and sporozoites of 3D7/NF54 is a single variant of the RIFIN protein family. This discovery may lead to the identification of the parasites binding ligands responsible for the adhesion during sexual stages and potentially to novel vaccine candidates.
U2 - 10.1186/1475-2875-9-147
DO - 10.1186/1475-2875-9-147
M3 - Journal article
C2 - 20509952
VL - 9
SP - 147
JO - Malaria Journal
JF - Malaria Journal
SN - 1475-2875
IS - 1
ER -
ID: 20121851