Identification and localization of a soluble antigen, Ag2, of 136 kDa from Plasmodium falciparum in vitro cultures

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Identification and localization of a soluble antigen, Ag2, of 136 kDa from Plasmodium falciparum in vitro cultures. / Jakobsen, P H; Grellier, P; Theander, T G; Behrendt, N; Torii, M; Aikawa, M; Schrevel, J; Jepsen, S.

In: Acta Pathologica Microbiologica et Immunologica Scandinavica, Vol. 99, No. 2, 1991, p. 155-62.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Jakobsen, PH, Grellier, P, Theander, TG, Behrendt, N, Torii, M, Aikawa, M, Schrevel, J & Jepsen, S 1991, 'Identification and localization of a soluble antigen, Ag2, of 136 kDa from Plasmodium falciparum in vitro cultures', Acta Pathologica Microbiologica et Immunologica Scandinavica, vol. 99, no. 2, pp. 155-62.

APA

Jakobsen, P. H., Grellier, P., Theander, T. G., Behrendt, N., Torii, M., Aikawa, M., Schrevel, J., & Jepsen, S. (1991). Identification and localization of a soluble antigen, Ag2, of 136 kDa from Plasmodium falciparum in vitro cultures. Acta Pathologica Microbiologica et Immunologica Scandinavica, 99(2), 155-62.

Vancouver

Jakobsen PH, Grellier P, Theander TG, Behrendt N, Torii M, Aikawa M et al. Identification and localization of a soluble antigen, Ag2, of 136 kDa from Plasmodium falciparum in vitro cultures. Acta Pathologica Microbiologica et Immunologica Scandinavica. 1991;99(2):155-62.

Author

Jakobsen, P H ; Grellier, P ; Theander, T G ; Behrendt, N ; Torii, M ; Aikawa, M ; Schrevel, J ; Jepsen, S. / Identification and localization of a soluble antigen, Ag2, of 136 kDa from Plasmodium falciparum in vitro cultures. In: Acta Pathologica Microbiologica et Immunologica Scandinavica. 1991 ; Vol. 99, No. 2. pp. 155-62.

Bibtex

@article{07222370a0dc11dd86a6000ea68e967b,
title = "Identification and localization of a soluble antigen, Ag2, of 136 kDa from Plasmodium falciparum in vitro cultures",
abstract = "The soluble antigens, antigen 2 (Ag2) and antigen 6 (Ag6), were copurified from supernatants of P. falciparum in vitro cultures by affinity chromatography and Fast Protein Liquid Chromatography. Rabbit antibodies to Ag2 were raised and characterized by crossed immunoelectrophoresis. Ag2 appeared as a duplet with molecular masses of 136 and 120 kDa when tested by immunoblotting. Immunoprecipitation experiments on Triton X-100 extracted antigens from synchronized cultures showed that the antigen was synthesized in the schizont stage. Ag2 was located near the surface of schizonts in the parasitophorous vacuole and in clefts in the infected erythrocyte cytoplasma as shown by immunogold electron microscopy.",
author = "Jakobsen, {P H} and P Grellier and Theander, {T G} and N Behrendt and M Torii and M Aikawa and J Schrevel and S Jepsen",
note = "Keywords: Adult; Animals; Antigens, Protozoan; Counterimmunoelectrophoresis; Humans; Immunoblotting; Plasmodium falciparum; Solubility",
year = "1991",
language = "English",
volume = "99",
pages = "155--62",
journal = "A P M I S. Acta Pathologica, Microbiologica et Immunologica Scandinavica",
issn = "0903-4641",
publisher = "Wiley Online",
number = "2",

}

RIS

TY - JOUR

T1 - Identification and localization of a soluble antigen, Ag2, of 136 kDa from Plasmodium falciparum in vitro cultures

AU - Jakobsen, P H

AU - Grellier, P

AU - Theander, T G

AU - Behrendt, N

AU - Torii, M

AU - Aikawa, M

AU - Schrevel, J

AU - Jepsen, S

N1 - Keywords: Adult; Animals; Antigens, Protozoan; Counterimmunoelectrophoresis; Humans; Immunoblotting; Plasmodium falciparum; Solubility

PY - 1991

Y1 - 1991

N2 - The soluble antigens, antigen 2 (Ag2) and antigen 6 (Ag6), were copurified from supernatants of P. falciparum in vitro cultures by affinity chromatography and Fast Protein Liquid Chromatography. Rabbit antibodies to Ag2 were raised and characterized by crossed immunoelectrophoresis. Ag2 appeared as a duplet with molecular masses of 136 and 120 kDa when tested by immunoblotting. Immunoprecipitation experiments on Triton X-100 extracted antigens from synchronized cultures showed that the antigen was synthesized in the schizont stage. Ag2 was located near the surface of schizonts in the parasitophorous vacuole and in clefts in the infected erythrocyte cytoplasma as shown by immunogold electron microscopy.

AB - The soluble antigens, antigen 2 (Ag2) and antigen 6 (Ag6), were copurified from supernatants of P. falciparum in vitro cultures by affinity chromatography and Fast Protein Liquid Chromatography. Rabbit antibodies to Ag2 were raised and characterized by crossed immunoelectrophoresis. Ag2 appeared as a duplet with molecular masses of 136 and 120 kDa when tested by immunoblotting. Immunoprecipitation experiments on Triton X-100 extracted antigens from synchronized cultures showed that the antigen was synthesized in the schizont stage. Ag2 was located near the surface of schizonts in the parasitophorous vacuole and in clefts in the infected erythrocyte cytoplasma as shown by immunogold electron microscopy.

M3 - Journal article

C2 - 2001282

VL - 99

SP - 155

EP - 162

JO - A P M I S. Acta Pathologica, Microbiologica et Immunologica Scandinavica

JF - A P M I S. Acta Pathologica, Microbiologica et Immunologica Scandinavica

SN - 0903-4641

IS - 2

ER -

ID: 6766936