Hospital epidemiology of methicillin-resistant Staphylococcus aureus in a tertiary care hospital in Moshi, Tanzania, as determined by whole genome sequencing

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Hospital epidemiology of methicillin-resistant Staphylococcus aureus in a tertiary care hospital in Moshi, Tanzania, as determined by whole genome sequencing. / Kumburu, Happiness H.; Sonda, Tolbert; Leekitcharoenphon, Pimlapas; van Zwetselaar, Marco; Lukjancenko, Oksana; Alifrangis, Michael; Lund, Ole; Mmbaga, Blandina T.; Kibiki, Gibson; Aarestrup, Frank M.

In: Journal of Biomedicine and Biotechnology, Vol. 2018, 2087693, 2018.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Kumburu, HH, Sonda, T, Leekitcharoenphon, P, van Zwetselaar, M, Lukjancenko, O, Alifrangis, M, Lund, O, Mmbaga, BT, Kibiki, G & Aarestrup, FM 2018, 'Hospital epidemiology of methicillin-resistant Staphylococcus aureus in a tertiary care hospital in Moshi, Tanzania, as determined by whole genome sequencing', Journal of Biomedicine and Biotechnology, vol. 2018, 2087693. https://doi.org/10.1155/2018/2087693

APA

Kumburu, H. H., Sonda, T., Leekitcharoenphon, P., van Zwetselaar, M., Lukjancenko, O., Alifrangis, M., Lund, O., Mmbaga, B. T., Kibiki, G., & Aarestrup, F. M. (2018). Hospital epidemiology of methicillin-resistant Staphylococcus aureus in a tertiary care hospital in Moshi, Tanzania, as determined by whole genome sequencing. Journal of Biomedicine and Biotechnology, 2018, [2087693]. https://doi.org/10.1155/2018/2087693

Vancouver

Kumburu HH, Sonda T, Leekitcharoenphon P, van Zwetselaar M, Lukjancenko O, Alifrangis M et al. Hospital epidemiology of methicillin-resistant Staphylococcus aureus in a tertiary care hospital in Moshi, Tanzania, as determined by whole genome sequencing. Journal of Biomedicine and Biotechnology. 2018;2018. 2087693. https://doi.org/10.1155/2018/2087693

Author

Kumburu, Happiness H. ; Sonda, Tolbert ; Leekitcharoenphon, Pimlapas ; van Zwetselaar, Marco ; Lukjancenko, Oksana ; Alifrangis, Michael ; Lund, Ole ; Mmbaga, Blandina T. ; Kibiki, Gibson ; Aarestrup, Frank M. / Hospital epidemiology of methicillin-resistant Staphylococcus aureus in a tertiary care hospital in Moshi, Tanzania, as determined by whole genome sequencing. In: Journal of Biomedicine and Biotechnology. 2018 ; Vol. 2018.

Bibtex

@article{8228dbd6dd0e4ce5bfb02bda5fa5ac47,
title = "Hospital epidemiology of methicillin-resistant Staphylococcus aureus in a tertiary care hospital in Moshi, Tanzania, as determined by whole genome sequencing",
abstract = "Objective. To determine molecular epidemiology of methicillin-resistant S. aureus in Tanzania using whole genome sequencing. Methods. DNA from 33 Staphylococcus species was recovered from subcultured archived Staphylococcus isolates. Whole genome sequencing was performed on Illumina Miseq using paired-end  bp protocol. Raw sequence data were analyzed using online tools. Results. Full susceptibility to vancomycin and chloramphenicol was observed. Thirteen isolates (43.3%) resisted cefoxitin and other antimicrobials tested. Multilocus sequence typing revealed 13 different sequence types among the 30 S. aureus isolates, with ST-8 ( = seven, 23%) being the most common. Gene detection in S. aureus stains were as follows: mecA, 10 (33.3%); pvl, 5 (16.7%); tst, 2 (6.7%). The SNP difference among the six Tanzanian ST-8 MRSA isolates ranged from 24 to 196 SNPs and from 16 to 446 SNPs when using the USA300_FPR3757 or the USA500_2395 as a reference, respectively. The mutation rate was SNPs/site/year or SNPs/site/year as estimated by USA300_FPR3757 or the USA500_2395, respectively. Conclusion. S. aureus isolates causing infections in hospitalized patients in Moshi are highly diverse and epidemiologically unrelated. Temporal phylogenetic analysis provided better resolution on transmission and introduction of MRSA and it may be important to include this in future routines.",
author = "Kumburu, {Happiness H.} and Tolbert Sonda and Pimlapas Leekitcharoenphon and {van Zwetselaar}, Marco and Oksana Lukjancenko and Michael Alifrangis and Ole Lund and Mmbaga, {Blandina T.} and Gibson Kibiki and Aarestrup, {Frank M.}",
year = "2018",
doi = "10.1155/2018/2087693",
language = "English",
volume = "2018",
journal = "BioMed Research International",
issn = "2314-6133",
publisher = "Hindawi Publishing Corporation",

}

RIS

TY - JOUR

T1 - Hospital epidemiology of methicillin-resistant Staphylococcus aureus in a tertiary care hospital in Moshi, Tanzania, as determined by whole genome sequencing

AU - Kumburu, Happiness H.

AU - Sonda, Tolbert

AU - Leekitcharoenphon, Pimlapas

AU - van Zwetselaar, Marco

AU - Lukjancenko, Oksana

AU - Alifrangis, Michael

AU - Lund, Ole

AU - Mmbaga, Blandina T.

AU - Kibiki, Gibson

AU - Aarestrup, Frank M.

PY - 2018

Y1 - 2018

N2 - Objective. To determine molecular epidemiology of methicillin-resistant S. aureus in Tanzania using whole genome sequencing. Methods. DNA from 33 Staphylococcus species was recovered from subcultured archived Staphylococcus isolates. Whole genome sequencing was performed on Illumina Miseq using paired-end  bp protocol. Raw sequence data were analyzed using online tools. Results. Full susceptibility to vancomycin and chloramphenicol was observed. Thirteen isolates (43.3%) resisted cefoxitin and other antimicrobials tested. Multilocus sequence typing revealed 13 different sequence types among the 30 S. aureus isolates, with ST-8 ( = seven, 23%) being the most common. Gene detection in S. aureus stains were as follows: mecA, 10 (33.3%); pvl, 5 (16.7%); tst, 2 (6.7%). The SNP difference among the six Tanzanian ST-8 MRSA isolates ranged from 24 to 196 SNPs and from 16 to 446 SNPs when using the USA300_FPR3757 or the USA500_2395 as a reference, respectively. The mutation rate was SNPs/site/year or SNPs/site/year as estimated by USA300_FPR3757 or the USA500_2395, respectively. Conclusion. S. aureus isolates causing infections in hospitalized patients in Moshi are highly diverse and epidemiologically unrelated. Temporal phylogenetic analysis provided better resolution on transmission and introduction of MRSA and it may be important to include this in future routines.

AB - Objective. To determine molecular epidemiology of methicillin-resistant S. aureus in Tanzania using whole genome sequencing. Methods. DNA from 33 Staphylococcus species was recovered from subcultured archived Staphylococcus isolates. Whole genome sequencing was performed on Illumina Miseq using paired-end  bp protocol. Raw sequence data were analyzed using online tools. Results. Full susceptibility to vancomycin and chloramphenicol was observed. Thirteen isolates (43.3%) resisted cefoxitin and other antimicrobials tested. Multilocus sequence typing revealed 13 different sequence types among the 30 S. aureus isolates, with ST-8 ( = seven, 23%) being the most common. Gene detection in S. aureus stains were as follows: mecA, 10 (33.3%); pvl, 5 (16.7%); tst, 2 (6.7%). The SNP difference among the six Tanzanian ST-8 MRSA isolates ranged from 24 to 196 SNPs and from 16 to 446 SNPs when using the USA300_FPR3757 or the USA500_2395 as a reference, respectively. The mutation rate was SNPs/site/year or SNPs/site/year as estimated by USA300_FPR3757 or the USA500_2395, respectively. Conclusion. S. aureus isolates causing infections in hospitalized patients in Moshi are highly diverse and epidemiologically unrelated. Temporal phylogenetic analysis provided better resolution on transmission and introduction of MRSA and it may be important to include this in future routines.

U2 - 10.1155/2018/2087693

DO - 10.1155/2018/2087693

M3 - Journal article

C2 - 29487865

VL - 2018

JO - BioMed Research International

JF - BioMed Research International

SN - 2314-6133

M1 - 2087693

ER -

ID: 188481157