Genetic polymorphisms in the glutamate-rich protein of Plasmodium falciparum field isolates from a malaria-endemic area of Brazil

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Standard

Genetic polymorphisms in the glutamate-rich protein of Plasmodium falciparum field isolates from a malaria-endemic area of Brazil. / Pratt-Riccio, Lilian Rose; Perce-da-Silva, Daiana de Souza; Lima-Junior, Josué da Costa; Theisen, Michael; Santos, Fátima; Daniel-Ribeiro, Cláudio Tadeu; de Oliveira-Ferreira, Joseli; Banic, Dalma Maria.

In: Memórias do Instituto Oswaldo Cruz, Vol. 108, No. 4, 06.2013, p. 523-8.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Pratt-Riccio, LR, Perce-da-Silva, DDS, Lima-Junior, JDC, Theisen, M, Santos, F, Daniel-Ribeiro, CT, de Oliveira-Ferreira, J & Banic, DM 2013, 'Genetic polymorphisms in the glutamate-rich protein of Plasmodium falciparum field isolates from a malaria-endemic area of Brazil', Memórias do Instituto Oswaldo Cruz, vol. 108, no. 4, pp. 523-8. https://doi.org/10.1590/S0074-02762013000400022

APA

Pratt-Riccio, L. R., Perce-da-Silva, D. D. S., Lima-Junior, J. D. C., Theisen, M., Santos, F., Daniel-Ribeiro, C. T., de Oliveira-Ferreira, J., & Banic, D. M. (2013). Genetic polymorphisms in the glutamate-rich protein of Plasmodium falciparum field isolates from a malaria-endemic area of Brazil. Memórias do Instituto Oswaldo Cruz, 108(4), 523-8. https://doi.org/10.1590/S0074-02762013000400022

Vancouver

Pratt-Riccio LR, Perce-da-Silva DDS, Lima-Junior JDC, Theisen M, Santos F, Daniel-Ribeiro CT et al. Genetic polymorphisms in the glutamate-rich protein of Plasmodium falciparum field isolates from a malaria-endemic area of Brazil. Memórias do Instituto Oswaldo Cruz. 2013 Jun;108(4):523-8. https://doi.org/10.1590/S0074-02762013000400022

Author

Pratt-Riccio, Lilian Rose ; Perce-da-Silva, Daiana de Souza ; Lima-Junior, Josué da Costa ; Theisen, Michael ; Santos, Fátima ; Daniel-Ribeiro, Cláudio Tadeu ; de Oliveira-Ferreira, Joseli ; Banic, Dalma Maria. / Genetic polymorphisms in the glutamate-rich protein of Plasmodium falciparum field isolates from a malaria-endemic area of Brazil. In: Memórias do Instituto Oswaldo Cruz. 2013 ; Vol. 108, No. 4. pp. 523-8.

Bibtex

@article{342898e2b9c444dba7caad4481989dd1,
title = "Genetic polymorphisms in the glutamate-rich protein of Plasmodium falciparum field isolates from a malaria-endemic area of Brazil",
abstract = "The genetic diversity displayed by Plasmodium falciparum, the most deadly Plasmodium species, is a significant obstacle for effective malaria vaccine development. In this study, we identified genetic polymorphisms in P. falciparum glutamate-rich protein (GLURP), which is currently being tested in clinical trials as a malaria vaccine candidate, from isolates found circulating in the Brazilian Amazon at variable transmission levels. The study was performed using samples collected in 1993 and 2008 from rural villages situated near Porto Velho, in the state of Rond{\^o}nia. DNA was extracted from 126 P. falciparum-positive thick blood smears using the phenol-chloroform method and subjected to a nested polymerase chain reaction protocol with specific primers against two immunodominant regions of GLURP, R0 and R2. Only one R0 fragment and four variants of the R2 fragment were detected. No differences were observed between the two time points with regard to the frequencies of the fragment variants. Mixed infections were uncommon. Our results demonstrate conservation of GLURP-R0 and limited polymorphic variation of GLURP-R2 in P. falciparum isolates from individuals living in Porto Velho. This is an important finding, as genetic polymorphisms in B and T-cell epitopes could have implications for the immunological properties of the antigen.",
author = "Pratt-Riccio, {Lilian Rose} and Perce-da-Silva, {Daiana de Souza} and Lima-Junior, {Josu{\'e} da Costa} and Michael Theisen and F{\'a}tima Santos and Daniel-Ribeiro, {Cl{\'a}udio Tadeu} and {de Oliveira-Ferreira}, Joseli and Banic, {Dalma Maria}",
year = "2013",
month = jun,
doi = "10.1590/S0074-02762013000400022",
language = "English",
volume = "108",
pages = "523--8",
journal = "Mem{\'o}rias do Instituto Oswaldo Cruz",
issn = "0074-0276",
publisher = "Instituto Oswaldo Cruz, Minist{\'e}rio da Sa{\'u}de",
number = "4",

}

RIS

TY - JOUR

T1 - Genetic polymorphisms in the glutamate-rich protein of Plasmodium falciparum field isolates from a malaria-endemic area of Brazil

AU - Pratt-Riccio, Lilian Rose

AU - Perce-da-Silva, Daiana de Souza

AU - Lima-Junior, Josué da Costa

AU - Theisen, Michael

AU - Santos, Fátima

AU - Daniel-Ribeiro, Cláudio Tadeu

AU - de Oliveira-Ferreira, Joseli

AU - Banic, Dalma Maria

PY - 2013/6

Y1 - 2013/6

N2 - The genetic diversity displayed by Plasmodium falciparum, the most deadly Plasmodium species, is a significant obstacle for effective malaria vaccine development. In this study, we identified genetic polymorphisms in P. falciparum glutamate-rich protein (GLURP), which is currently being tested in clinical trials as a malaria vaccine candidate, from isolates found circulating in the Brazilian Amazon at variable transmission levels. The study was performed using samples collected in 1993 and 2008 from rural villages situated near Porto Velho, in the state of Rondônia. DNA was extracted from 126 P. falciparum-positive thick blood smears using the phenol-chloroform method and subjected to a nested polymerase chain reaction protocol with specific primers against two immunodominant regions of GLURP, R0 and R2. Only one R0 fragment and four variants of the R2 fragment were detected. No differences were observed between the two time points with regard to the frequencies of the fragment variants. Mixed infections were uncommon. Our results demonstrate conservation of GLURP-R0 and limited polymorphic variation of GLURP-R2 in P. falciparum isolates from individuals living in Porto Velho. This is an important finding, as genetic polymorphisms in B and T-cell epitopes could have implications for the immunological properties of the antigen.

AB - The genetic diversity displayed by Plasmodium falciparum, the most deadly Plasmodium species, is a significant obstacle for effective malaria vaccine development. In this study, we identified genetic polymorphisms in P. falciparum glutamate-rich protein (GLURP), which is currently being tested in clinical trials as a malaria vaccine candidate, from isolates found circulating in the Brazilian Amazon at variable transmission levels. The study was performed using samples collected in 1993 and 2008 from rural villages situated near Porto Velho, in the state of Rondônia. DNA was extracted from 126 P. falciparum-positive thick blood smears using the phenol-chloroform method and subjected to a nested polymerase chain reaction protocol with specific primers against two immunodominant regions of GLURP, R0 and R2. Only one R0 fragment and four variants of the R2 fragment were detected. No differences were observed between the two time points with regard to the frequencies of the fragment variants. Mixed infections were uncommon. Our results demonstrate conservation of GLURP-R0 and limited polymorphic variation of GLURP-R2 in P. falciparum isolates from individuals living in Porto Velho. This is an important finding, as genetic polymorphisms in B and T-cell epitopes could have implications for the immunological properties of the antigen.

U2 - 10.1590/S0074-02762013000400022

DO - 10.1590/S0074-02762013000400022

M3 - Journal article

C2 - 23828006

VL - 108

SP - 523

EP - 528

JO - Memórias do Instituto Oswaldo Cruz

JF - Memórias do Instituto Oswaldo Cruz

SN - 0074-0276

IS - 4

ER -

ID: 80640446