Development and evaluation of a multiplex screening assay for Plasmodium falciparum exposure

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Standard

Development and evaluation of a multiplex screening assay for Plasmodium falciparum exposure. / Jepsen, Micha Phill Grønholm; Röser, Dennis; Christiansen, Michael; Larsen, Severin Olesen; Cavanagh, David R; Dhanasarnsombut, Kelwalin; Bygbjerg, Ib; Dodoo, Daniel; Remarque, Edmond J; Dziegiel, Morten; Jepsen, Søren; Mordmüller, Benjamin; Theisen, Michael.

In: Journal of Immunological Methods, Vol. 384, No. 1-2, 2012, p. 62-70.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Jepsen, MPG, Röser, D, Christiansen, M, Larsen, SO, Cavanagh, DR, Dhanasarnsombut, K, Bygbjerg, I, Dodoo, D, Remarque, EJ, Dziegiel, M, Jepsen, S, Mordmüller, B & Theisen, M 2012, 'Development and evaluation of a multiplex screening assay for Plasmodium falciparum exposure', Journal of Immunological Methods, vol. 384, no. 1-2, pp. 62-70. https://doi.org/10.1016/j.jim.2012.07.009

APA

Jepsen, M. P. G., Röser, D., Christiansen, M., Larsen, S. O., Cavanagh, D. R., Dhanasarnsombut, K., Bygbjerg, I., Dodoo, D., Remarque, E. J., Dziegiel, M., Jepsen, S., Mordmüller, B., & Theisen, M. (2012). Development and evaluation of a multiplex screening assay for Plasmodium falciparum exposure. Journal of Immunological Methods, 384(1-2), 62-70. https://doi.org/10.1016/j.jim.2012.07.009

Vancouver

Jepsen MPG, Röser D, Christiansen M, Larsen SO, Cavanagh DR, Dhanasarnsombut K et al. Development and evaluation of a multiplex screening assay for Plasmodium falciparum exposure. Journal of Immunological Methods. 2012;384(1-2):62-70. https://doi.org/10.1016/j.jim.2012.07.009

Author

Jepsen, Micha Phill Grønholm ; Röser, Dennis ; Christiansen, Michael ; Larsen, Severin Olesen ; Cavanagh, David R ; Dhanasarnsombut, Kelwalin ; Bygbjerg, Ib ; Dodoo, Daniel ; Remarque, Edmond J ; Dziegiel, Morten ; Jepsen, Søren ; Mordmüller, Benjamin ; Theisen, Michael. / Development and evaluation of a multiplex screening assay for Plasmodium falciparum exposure. In: Journal of Immunological Methods. 2012 ; Vol. 384, No. 1-2. pp. 62-70.

Bibtex

@article{b4e9a6372a6343f3a84a7d2d4bc9cc86,
title = "Development and evaluation of a multiplex screening assay for Plasmodium falciparum exposure",
abstract = "Transfusion transmitted malaria (TTM) in non-endemic countries is reduced by questioning blood donors and screening of donated blood. Conventional screening is performed by Indirect Fluorescence Antibody Test (IFAT). This method is manual and difficult to standardize. Here we study the diagnostic performance of a multiplex assay for detection of antibodies against Plasmodium falciparum in donor blood using IFAT as a comparator. A multiplex assay (MPA) containing the antigens GLURP-R0, GLURP-R2, MSP3, MSP1 hybrid and AMA1 was constructed using xMAP{\textregistered} technology. A discrimination index for exposure to P. falciparum malaria was calculated by comparing travelers with clinical malaria (n=52) and non-exposed blood donors (n=119). The index was evaluated on blood donors with suspected malaria exposure (n=249) and compared to the diagnostic performance of IFAT. At a specificity of 95.8 %, the MPA discrimination index exhibited a diagnostic sensitivity of 90.4 % in travelers hospitalized with malaria. Percent agreement with IFAT was 92.3 %. Screening plasma from blood donors with suspected malaria exposure, we found 4.8 % to be positive by IFAT and 5.2 % by MPA with an agreement of 93.2 %. The calculated index from the MPA exhibits similar diagnostic performance as IFAT for detection of P. falciparum malaria. Combining the antibody response against multiple antigens in a discrimination index increased the sensitivity of the MPA and reduced the readout to a single value.",
author = "Jepsen, {Micha Phill Gr{\o}nholm} and Dennis R{\"o}ser and Michael Christiansen and Larsen, {Severin Olesen} and Cavanagh, {David R} and Kelwalin Dhanasarnsombut and Ib Bygbjerg and Daniel Dodoo and Remarque, {Edmond J} and Morten Dziegiel and S{\o}ren Jepsen and Benjamin Mordm{\"u}ller and Michael Theisen",
note = "Copyright {\textcopyright} 2012 Elsevier B.V. All rights reserved.",
year = "2012",
doi = "10.1016/j.jim.2012.07.009",
language = "English",
volume = "384",
pages = "62--70",
journal = "Journal of Immunological Methods",
issn = "0022-1759",
publisher = "Elsevier",
number = "1-2",

}

RIS

TY - JOUR

T1 - Development and evaluation of a multiplex screening assay for Plasmodium falciparum exposure

AU - Jepsen, Micha Phill Grønholm

AU - Röser, Dennis

AU - Christiansen, Michael

AU - Larsen, Severin Olesen

AU - Cavanagh, David R

AU - Dhanasarnsombut, Kelwalin

AU - Bygbjerg, Ib

AU - Dodoo, Daniel

AU - Remarque, Edmond J

AU - Dziegiel, Morten

AU - Jepsen, Søren

AU - Mordmüller, Benjamin

AU - Theisen, Michael

N1 - Copyright © 2012 Elsevier B.V. All rights reserved.

PY - 2012

Y1 - 2012

N2 - Transfusion transmitted malaria (TTM) in non-endemic countries is reduced by questioning blood donors and screening of donated blood. Conventional screening is performed by Indirect Fluorescence Antibody Test (IFAT). This method is manual and difficult to standardize. Here we study the diagnostic performance of a multiplex assay for detection of antibodies against Plasmodium falciparum in donor blood using IFAT as a comparator. A multiplex assay (MPA) containing the antigens GLURP-R0, GLURP-R2, MSP3, MSP1 hybrid and AMA1 was constructed using xMAP® technology. A discrimination index for exposure to P. falciparum malaria was calculated by comparing travelers with clinical malaria (n=52) and non-exposed blood donors (n=119). The index was evaluated on blood donors with suspected malaria exposure (n=249) and compared to the diagnostic performance of IFAT. At a specificity of 95.8 %, the MPA discrimination index exhibited a diagnostic sensitivity of 90.4 % in travelers hospitalized with malaria. Percent agreement with IFAT was 92.3 %. Screening plasma from blood donors with suspected malaria exposure, we found 4.8 % to be positive by IFAT and 5.2 % by MPA with an agreement of 93.2 %. The calculated index from the MPA exhibits similar diagnostic performance as IFAT for detection of P. falciparum malaria. Combining the antibody response against multiple antigens in a discrimination index increased the sensitivity of the MPA and reduced the readout to a single value.

AB - Transfusion transmitted malaria (TTM) in non-endemic countries is reduced by questioning blood donors and screening of donated blood. Conventional screening is performed by Indirect Fluorescence Antibody Test (IFAT). This method is manual and difficult to standardize. Here we study the diagnostic performance of a multiplex assay for detection of antibodies against Plasmodium falciparum in donor blood using IFAT as a comparator. A multiplex assay (MPA) containing the antigens GLURP-R0, GLURP-R2, MSP3, MSP1 hybrid and AMA1 was constructed using xMAP® technology. A discrimination index for exposure to P. falciparum malaria was calculated by comparing travelers with clinical malaria (n=52) and non-exposed blood donors (n=119). The index was evaluated on blood donors with suspected malaria exposure (n=249) and compared to the diagnostic performance of IFAT. At a specificity of 95.8 %, the MPA discrimination index exhibited a diagnostic sensitivity of 90.4 % in travelers hospitalized with malaria. Percent agreement with IFAT was 92.3 %. Screening plasma from blood donors with suspected malaria exposure, we found 4.8 % to be positive by IFAT and 5.2 % by MPA with an agreement of 93.2 %. The calculated index from the MPA exhibits similar diagnostic performance as IFAT for detection of P. falciparum malaria. Combining the antibody response against multiple antigens in a discrimination index increased the sensitivity of the MPA and reduced the readout to a single value.

U2 - 10.1016/j.jim.2012.07.009

DO - 10.1016/j.jim.2012.07.009

M3 - Journal article

C2 - 22835432

VL - 384

SP - 62

EP - 70

JO - Journal of Immunological Methods

JF - Journal of Immunological Methods

SN - 0022-1759

IS - 1-2

ER -

ID: 40479290