Cryo scanning electron microscopy of Plasmodium falciparum-infected erythrocytes

Research output: Contribution to journal › Journal article › Research › peer-review

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Cryo scanning electron microscopy of Plasmodium falciparum-infected erythrocytes. / Hempel, Casper.

In: APMIS : acta pathologica, microbiologica, et immunologica Scandinavica, Vol. 125, No. 7, 2017, p. 650-654.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Hempel, C 2017, 'Cryo scanning electron microscopy of Plasmodium falciparum-infected erythrocytes', APMIS : acta pathologica, microbiologica, et immunologica Scandinavica, vol. 125, no. 7, pp. 650-654. https://doi.org/10.1111/apm.12699

APA

Hempel, C. (2017). Cryo scanning electron microscopy of Plasmodium falciparum-infected erythrocytes. APMIS : acta pathologica, microbiologica, et immunologica Scandinavica, 125(7), 650-654. https://doi.org/10.1111/apm.12699

Vancouver

Hempel C. Cryo scanning electron microscopy of Plasmodium falciparum-infected erythrocytes. APMIS : acta pathologica, microbiologica, et immunologica Scandinavica. 2017;125(7):650-654. https://doi.org/10.1111/apm.12699

Author

Hempel, Casper. / Cryo scanning electron microscopy of Plasmodium falciparum-infected erythrocytes. In: APMIS : acta pathologica, microbiologica, et immunologica Scandinavica. 2017 ; Vol. 125, No. 7. pp. 650-654.

Bibtex

@article{2b601fb9fe9b4c6ba97f899afc98d956,
title = "Cryo scanning electron microscopy of Plasmodium falciparum-infected erythrocytes",
abstract = "Plasmodium falciparum invades erythrocytes as an essential part of their life cycle. While living inside erythrocytes, the parasite remodels the cell's intracellular organization as well as its outer surface. Late trophozoite-stage parasites and schizonts introduce numerous small protrusions on the erythrocyte surface, called knobs. Current methods for studying these knobs include atomic force microscopy and electron microscopy. Standard electron microscopy methods rely on chemical fixation and dehydration modifying cell size. Here, a novel method is presented using rapid freezing and scanning electron microscopy under cryogenic conditions allowing for high resolution and magnification of erythrocytes. This novel technique can be used for precise estimates of knob density and for studies on cytoadhesion.",
author = "Casper Hempel",
note = "{\textcopyright} 2017 APMIS. Published by John Wiley & Sons Ltd.",
year = "2017",
doi = "10.1111/apm.12699",
language = "English",
volume = "125",
pages = "650--654",
journal = "A P M I S. Acta Pathologica, Microbiologica et Immunologica Scandinavica",
issn = "0903-4641",
publisher = "Wiley Online",
number = "7",

}

RIS

TY - JOUR

T1 - Cryo scanning electron microscopy of Plasmodium falciparum-infected erythrocytes

AU - Hempel, Casper

N1 - © 2017 APMIS. Published by John Wiley & Sons Ltd.

PY - 2017

Y1 - 2017

N2 - Plasmodium falciparum invades erythrocytes as an essential part of their life cycle. While living inside erythrocytes, the parasite remodels the cell's intracellular organization as well as its outer surface. Late trophozoite-stage parasites and schizonts introduce numerous small protrusions on the erythrocyte surface, called knobs. Current methods for studying these knobs include atomic force microscopy and electron microscopy. Standard electron microscopy methods rely on chemical fixation and dehydration modifying cell size. Here, a novel method is presented using rapid freezing and scanning electron microscopy under cryogenic conditions allowing for high resolution and magnification of erythrocytes. This novel technique can be used for precise estimates of knob density and for studies on cytoadhesion.

AB - Plasmodium falciparum invades erythrocytes as an essential part of their life cycle. While living inside erythrocytes, the parasite remodels the cell's intracellular organization as well as its outer surface. Late trophozoite-stage parasites and schizonts introduce numerous small protrusions on the erythrocyte surface, called knobs. Current methods for studying these knobs include atomic force microscopy and electron microscopy. Standard electron microscopy methods rely on chemical fixation and dehydration modifying cell size. Here, a novel method is presented using rapid freezing and scanning electron microscopy under cryogenic conditions allowing for high resolution and magnification of erythrocytes. This novel technique can be used for precise estimates of knob density and for studies on cytoadhesion.

U2 - 10.1111/apm.12699

DO - 10.1111/apm.12699

M3 - Journal article

C2 - 28493454

VL - 125

SP - 650

EP - 654

JO - A P M I S. Acta Pathologica, Microbiologica et Immunologica Scandinavica

JF - A P M I S. Acta Pathologica, Microbiologica et Immunologica Scandinavica

SN - 0903-4641

IS - 7

ER -

ID: 179522016