Computer-assisted image analysis assay of human neutrophil chemotaxis in vitro
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Computer-assisted image analysis assay of human neutrophil chemotaxis in vitro. / Jensen, P; Kharazmi, A.
In: Journal of Immunological Methods, Vol. 144, No. 1, 1991, p. 43-8.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Computer-assisted image analysis assay of human neutrophil chemotaxis in vitro
AU - Jensen, P
AU - Kharazmi, A
N1 - Keywords: Chemotaxis, Leukocyte; Humans; Image Processing, Computer-Assisted; N-Formylmethionine Leucyl-Phenylalanine; Neutrophils; Zymosan
PY - 1991
Y1 - 1991
N2 - We have developed a computer-based image analysis system to measure in-filter migration of human neutrophils in the Boyden chamber. This method is compared with the conventional manual counting techniques. Neutrophils from healthy individuals and from patients with reduced chemotactic activity were used. The cells migrating into the filter were counted automatically at depths 20 microns apart commencing from the upper surface on the filter. The major advantages of this method are reproducibility and the counting of many fields which provides better data for statistical analysis of the results. Another advantage of the assay is that it can be used to show the migration pattern of different populations of neutrophils from both healthy individuals and patients.
AB - We have developed a computer-based image analysis system to measure in-filter migration of human neutrophils in the Boyden chamber. This method is compared with the conventional manual counting techniques. Neutrophils from healthy individuals and from patients with reduced chemotactic activity were used. The cells migrating into the filter were counted automatically at depths 20 microns apart commencing from the upper surface on the filter. The major advantages of this method are reproducibility and the counting of many fields which provides better data for statistical analysis of the results. Another advantage of the assay is that it can be used to show the migration pattern of different populations of neutrophils from both healthy individuals and patients.
M3 - Journal article
C2 - 1960405
VL - 144
SP - 43
EP - 48
JO - Journal of Immunological Methods
JF - Journal of Immunological Methods
SN - 0022-1759
IS - 1
ER -
ID: 18177830