Antibodies against the Plasmodium falciparum glutamate-rich protein from naturally exposed individuals living in a Brazilian malaria-endemic area can inhibit in vitro parasite growth

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Antibodies against the Plasmodium falciparum glutamate-rich protein from naturally exposed individuals living in a Brazilian malaria-endemic area can inhibit in vitro parasite growth. / Pratt-Riccio, Lilian Rose; Bianco, Cesare; Totino, Paulo Renato Rivas; Perce-Da-Silva, Daiana De Souza; Silva, Luciene Aquino; Riccio, Evelyn Kety Pratt; Ennes-Vidal, Vítor; Neves-Ferreira, Ana Gisele Costa; Perales, Jonas; Da Rocha, Surza Lucia Gonçalves; Dias-Da-Silva, Fabrício; Ferreira-da-Cruz, Maria de Fátima; Daniel-Ribeiro, Cláudio Tadeu; De Oliveira-Ferreira, Joseli; Theisen, Michael; Carvalho, Leonardo José De Moura; Banic, Dalma Maria.

In: Memórias do Instituto Oswaldo Cruz, Vol. 106 Suppl 1, 2011, p. 34-43.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Pratt-Riccio, LR, Bianco, C, Totino, PRR, Perce-Da-Silva, DDS, Silva, LA, Riccio, EKP, Ennes-Vidal, V, Neves-Ferreira, AGC, Perales, J, Da Rocha, SLG, Dias-Da-Silva, F, Ferreira-da-Cruz, MDF, Daniel-Ribeiro, CT, De Oliveira-Ferreira, J, Theisen, M, Carvalho, LJDM & Banic, DM 2011, 'Antibodies against the Plasmodium falciparum glutamate-rich protein from naturally exposed individuals living in a Brazilian malaria-endemic area can inhibit in vitro parasite growth', Memórias do Instituto Oswaldo Cruz, vol. 106 Suppl 1, pp. 34-43.

APA

Pratt-Riccio, L. R., Bianco, C., Totino, P. R. R., Perce-Da-Silva, D. D. S., Silva, L. A., Riccio, E. K. P., Ennes-Vidal, V., Neves-Ferreira, A. G. C., Perales, J., Da Rocha, S. L. G., Dias-Da-Silva, F., Ferreira-da-Cruz, M. D. F., Daniel-Ribeiro, C. T., De Oliveira-Ferreira, J., Theisen, M., Carvalho, L. J. D. M., & Banic, D. M. (2011). Antibodies against the Plasmodium falciparum glutamate-rich protein from naturally exposed individuals living in a Brazilian malaria-endemic area can inhibit in vitro parasite growth. Memórias do Instituto Oswaldo Cruz, 106 Suppl 1, 34-43.

Vancouver

Pratt-Riccio LR, Bianco C, Totino PRR, Perce-Da-Silva DDS, Silva LA, Riccio EKP et al. Antibodies against the Plasmodium falciparum glutamate-rich protein from naturally exposed individuals living in a Brazilian malaria-endemic area can inhibit in vitro parasite growth. Memórias do Instituto Oswaldo Cruz. 2011;106 Suppl 1:34-43.

Author

Pratt-Riccio, Lilian Rose ; Bianco, Cesare ; Totino, Paulo Renato Rivas ; Perce-Da-Silva, Daiana De Souza ; Silva, Luciene Aquino ; Riccio, Evelyn Kety Pratt ; Ennes-Vidal, Vítor ; Neves-Ferreira, Ana Gisele Costa ; Perales, Jonas ; Da Rocha, Surza Lucia Gonçalves ; Dias-Da-Silva, Fabrício ; Ferreira-da-Cruz, Maria de Fátima ; Daniel-Ribeiro, Cláudio Tadeu ; De Oliveira-Ferreira, Joseli ; Theisen, Michael ; Carvalho, Leonardo José De Moura ; Banic, Dalma Maria. / Antibodies against the Plasmodium falciparum glutamate-rich protein from naturally exposed individuals living in a Brazilian malaria-endemic area can inhibit in vitro parasite growth. In: Memórias do Instituto Oswaldo Cruz. 2011 ; Vol. 106 Suppl 1. pp. 34-43.

Bibtex

@article{e32eb934cca247cd892750ab3af24848,
title = "Antibodies against the Plasmodium falciparum glutamate-rich protein from naturally exposed individuals living in a Brazilian malaria-endemic area can inhibit in vitro parasite growth",
abstract = "The glutamate-rich protein (GLURP) is an exoantigen expressed in all stages of the Plasmodium falciparum life cycle in humans. Anti-GLURP antibodies can inhibit parasite growth in the presence of monocytes via antibody-dependent cellular inhibition (ADCI), and a major parasite-inhibitory region has been found in the N-terminal R0 region of the protein. Herein, we describe the antiplasmodial activity of anti-GLURP antibodies present in the sera from individuals naturally exposed to malaria in a Brazilian malaria-endemic area. The anti-R0 antibodies showed a potent inhibitory effect on the growth of P. falciparum in vitro, both in the presence (ADCI) and absence (GI) of monocytes. The inhibitory effect on parasite growth was comparable to the effect of IgGs purified from pooled sera from hyperimmune African individuals. Interestingly, in the ADCI test, higher levels of tumour necrosis factor alpha (TNF-a) were observed in the supernatant from cultures with higher parasitemias. Our data suggest that the antibody response induced by GLURP-R0 in naturally exposed individuals may have an important role in controlling parasitemia because these antibodies are able to inhibit the in vitro growth of P. falciparum with or without the cooperation from monocytes. Our results also indicate that TNF-a may not be relevant for the inhibitory effect on P. falciparum in vitro growth.",
keywords = "Adolescent, Adult, Aged, Antibodies, Protozoan, Endemic Diseases, Enzyme-Linked Immunosorbent Assay, Fluorescent Antibody Technique, Indirect, Humans, Immunoglobulin G, Malaria, Falciparum, Middle Aged, Parasitemia, Plasmodium falciparum, Protozoan Proteins, Tumor Necrosis Factor-alpha, Young Adult",
author = "Pratt-Riccio, {Lilian Rose} and Cesare Bianco and Totino, {Paulo Renato Rivas} and Perce-Da-Silva, {Daiana De Souza} and Silva, {Luciene Aquino} and Riccio, {Evelyn Kety Pratt} and V{\'i}tor Ennes-Vidal and Neves-Ferreira, {Ana Gisele Costa} and Jonas Perales and {Da Rocha}, {Surza Lucia Gon{\c c}alves} and Fabr{\'i}cio Dias-Da-Silva and Ferreira-da-Cruz, {Maria de F{\'a}tima} and Daniel-Ribeiro, {Cl{\'a}udio Tadeu} and {De Oliveira-Ferreira}, Joseli and Michael Theisen and Carvalho, {Leonardo Jos{\'e} De Moura} and Banic, {Dalma Maria}",
year = "2011",
language = "English",
volume = "106 Suppl 1",
pages = "34--43",
journal = "Mem{\'o}rias do Instituto Oswaldo Cruz",
issn = "0074-0276",
publisher = "Instituto Oswaldo Cruz, Minist{\'e}rio da Sa{\'u}de",

}

RIS

TY - JOUR

T1 - Antibodies against the Plasmodium falciparum glutamate-rich protein from naturally exposed individuals living in a Brazilian malaria-endemic area can inhibit in vitro parasite growth

AU - Pratt-Riccio, Lilian Rose

AU - Bianco, Cesare

AU - Totino, Paulo Renato Rivas

AU - Perce-Da-Silva, Daiana De Souza

AU - Silva, Luciene Aquino

AU - Riccio, Evelyn Kety Pratt

AU - Ennes-Vidal, Vítor

AU - Neves-Ferreira, Ana Gisele Costa

AU - Perales, Jonas

AU - Da Rocha, Surza Lucia Gonçalves

AU - Dias-Da-Silva, Fabrício

AU - Ferreira-da-Cruz, Maria de Fátima

AU - Daniel-Ribeiro, Cláudio Tadeu

AU - De Oliveira-Ferreira, Joseli

AU - Theisen, Michael

AU - Carvalho, Leonardo José De Moura

AU - Banic, Dalma Maria

PY - 2011

Y1 - 2011

N2 - The glutamate-rich protein (GLURP) is an exoantigen expressed in all stages of the Plasmodium falciparum life cycle in humans. Anti-GLURP antibodies can inhibit parasite growth in the presence of monocytes via antibody-dependent cellular inhibition (ADCI), and a major parasite-inhibitory region has been found in the N-terminal R0 region of the protein. Herein, we describe the antiplasmodial activity of anti-GLURP antibodies present in the sera from individuals naturally exposed to malaria in a Brazilian malaria-endemic area. The anti-R0 antibodies showed a potent inhibitory effect on the growth of P. falciparum in vitro, both in the presence (ADCI) and absence (GI) of monocytes. The inhibitory effect on parasite growth was comparable to the effect of IgGs purified from pooled sera from hyperimmune African individuals. Interestingly, in the ADCI test, higher levels of tumour necrosis factor alpha (TNF-a) were observed in the supernatant from cultures with higher parasitemias. Our data suggest that the antibody response induced by GLURP-R0 in naturally exposed individuals may have an important role in controlling parasitemia because these antibodies are able to inhibit the in vitro growth of P. falciparum with or without the cooperation from monocytes. Our results also indicate that TNF-a may not be relevant for the inhibitory effect on P. falciparum in vitro growth.

AB - The glutamate-rich protein (GLURP) is an exoantigen expressed in all stages of the Plasmodium falciparum life cycle in humans. Anti-GLURP antibodies can inhibit parasite growth in the presence of monocytes via antibody-dependent cellular inhibition (ADCI), and a major parasite-inhibitory region has been found in the N-terminal R0 region of the protein. Herein, we describe the antiplasmodial activity of anti-GLURP antibodies present in the sera from individuals naturally exposed to malaria in a Brazilian malaria-endemic area. The anti-R0 antibodies showed a potent inhibitory effect on the growth of P. falciparum in vitro, both in the presence (ADCI) and absence (GI) of monocytes. The inhibitory effect on parasite growth was comparable to the effect of IgGs purified from pooled sera from hyperimmune African individuals. Interestingly, in the ADCI test, higher levels of tumour necrosis factor alpha (TNF-a) were observed in the supernatant from cultures with higher parasitemias. Our data suggest that the antibody response induced by GLURP-R0 in naturally exposed individuals may have an important role in controlling parasitemia because these antibodies are able to inhibit the in vitro growth of P. falciparum with or without the cooperation from monocytes. Our results also indicate that TNF-a may not be relevant for the inhibitory effect on P. falciparum in vitro growth.

KW - Adolescent

KW - Adult

KW - Aged

KW - Antibodies, Protozoan

KW - Endemic Diseases

KW - Enzyme-Linked Immunosorbent Assay

KW - Fluorescent Antibody Technique, Indirect

KW - Humans

KW - Immunoglobulin G

KW - Malaria, Falciparum

KW - Middle Aged

KW - Parasitemia

KW - Plasmodium falciparum

KW - Protozoan Proteins

KW - Tumor Necrosis Factor-alpha

KW - Young Adult

M3 - Journal article

C2 - 21881755

VL - 106 Suppl 1

SP - 34

EP - 43

JO - Memórias do Instituto Oswaldo Cruz

JF - Memórias do Instituto Oswaldo Cruz

SN - 0074-0276

ER -

ID: 37451778