Analysis of var gene transcript patterns by quantitative real-time PCR

Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review

Standard

Analysis of var gene transcript patterns by quantitative real-time PCR. / Bachmann, Anna; Lavstsen, Thomas.

Malaria Immunology: Targeting the Surface of Infected Erythrocytes. Vol. 2470 Humana Press, 2022. p. 149–171 (Methods in molecular biology (Clifton, N.J.)).

Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review

Harvard

Bachmann, A & Lavstsen, T 2022, Analysis of var gene transcript patterns by quantitative real-time PCR. in Malaria Immunology: Targeting the Surface of Infected Erythrocytes. vol. 2470, Humana Press, Methods in molecular biology (Clifton, N.J.), pp. 149–171. https://doi.org/10.1007/978-1-0716-2189-9_13

APA

Bachmann, A., & Lavstsen, T. (2022). Analysis of var gene transcript patterns by quantitative real-time PCR. In Malaria Immunology: Targeting the Surface of Infected Erythrocytes (Vol. 2470, pp. 149–171). Humana Press. Methods in molecular biology (Clifton, N.J.) https://doi.org/10.1007/978-1-0716-2189-9_13

Vancouver

Bachmann A, Lavstsen T. Analysis of var gene transcript patterns by quantitative real-time PCR. In Malaria Immunology: Targeting the Surface of Infected Erythrocytes. Vol. 2470. Humana Press. 2022. p. 149–171. (Methods in molecular biology (Clifton, N.J.)). https://doi.org/10.1007/978-1-0716-2189-9_13

Author

Bachmann, Anna ; Lavstsen, Thomas. / Analysis of var gene transcript patterns by quantitative real-time PCR. Malaria Immunology: Targeting the Surface of Infected Erythrocytes. Vol. 2470 Humana Press, 2022. pp. 149–171 (Methods in molecular biology (Clifton, N.J.)).

Bibtex

@inbook{1c3b5ec840f44c0f89f0a9c1218aeab8,
title = "Analysis of var gene transcript patterns by quantitative real-time PCR",
abstract = "Quantitative real-time PCR (qPCR) is a simple and sensitive method for determining the amount of a specific target DNA sequence present in a sample. Compared to RNA-seq, reverse transcription qPCR (RT-qPCR) is fast, requires only low input material and is easy to analyze. Therefore, qPCR is widely used to analyze gene expression in P. falciparum, including analyses of the multicopy gene families encoding variant surface antigens (VSAs), whose expression is clonally variant and prone to changes over time. In the recent years, several P. falciparum genomes of culture-adapted strains have been sequenced, providing the knowledge to design variable gene family-specific qPCR primers for each P. falciparum genetic background. Here, we describe the required materials, methods and key factors to perform RT-qPCR experiments to determine VSA transcript abundances in the P. falciparum clones 3D7/NF54, IT4, HB3, and 7G8.",
keywords = "Genes, Protozoan, Humans, Malaria, Falciparum, Plasmodium falciparum/metabolism, Protozoan Proteins/genetics, Real-Time Polymerase Chain Reaction",
author = "Anna Bachmann and Thomas Lavstsen",
note = "{\textcopyright} 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2022",
doi = "10.1007/978-1-0716-2189-9_13",
language = "English",
volume = "2470",
series = "Methods in molecular biology (Clifton, N.J.)",
publisher = "Humana Press",
pages = "149–171",
booktitle = "Malaria Immunology",
address = "United States",

}

RIS

TY - CHAP

T1 - Analysis of var gene transcript patterns by quantitative real-time PCR

AU - Bachmann, Anna

AU - Lavstsen, Thomas

N1 - © 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

PY - 2022

Y1 - 2022

N2 - Quantitative real-time PCR (qPCR) is a simple and sensitive method for determining the amount of a specific target DNA sequence present in a sample. Compared to RNA-seq, reverse transcription qPCR (RT-qPCR) is fast, requires only low input material and is easy to analyze. Therefore, qPCR is widely used to analyze gene expression in P. falciparum, including analyses of the multicopy gene families encoding variant surface antigens (VSAs), whose expression is clonally variant and prone to changes over time. In the recent years, several P. falciparum genomes of culture-adapted strains have been sequenced, providing the knowledge to design variable gene family-specific qPCR primers for each P. falciparum genetic background. Here, we describe the required materials, methods and key factors to perform RT-qPCR experiments to determine VSA transcript abundances in the P. falciparum clones 3D7/NF54, IT4, HB3, and 7G8.

AB - Quantitative real-time PCR (qPCR) is a simple and sensitive method for determining the amount of a specific target DNA sequence present in a sample. Compared to RNA-seq, reverse transcription qPCR (RT-qPCR) is fast, requires only low input material and is easy to analyze. Therefore, qPCR is widely used to analyze gene expression in P. falciparum, including analyses of the multicopy gene families encoding variant surface antigens (VSAs), whose expression is clonally variant and prone to changes over time. In the recent years, several P. falciparum genomes of culture-adapted strains have been sequenced, providing the knowledge to design variable gene family-specific qPCR primers for each P. falciparum genetic background. Here, we describe the required materials, methods and key factors to perform RT-qPCR experiments to determine VSA transcript abundances in the P. falciparum clones 3D7/NF54, IT4, HB3, and 7G8.

KW - Genes, Protozoan

KW - Humans

KW - Malaria, Falciparum

KW - Plasmodium falciparum/metabolism

KW - Protozoan Proteins/genetics

KW - Real-Time Polymerase Chain Reaction

U2 - 10.1007/978-1-0716-2189-9_13

DO - 10.1007/978-1-0716-2189-9_13

M3 - Book chapter

C2 - 35881345

VL - 2470

T3 - Methods in molecular biology (Clifton, N.J.)

SP - 149

EP - 171

BT - Malaria Immunology

PB - Humana Press

ER -

ID: 320649062