Analysis of antibody inhibition of PfEMP1 binding by competition ELISA

Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review

Plasmodium falciparum express variant antigens on the surface of infected erythrocytes (IEs). These include proteins of the P. falciparum erythrocyte membrane protein 1 (PfEMP1) family. PfEMP1 proteins mediate binding of IEs to various human endothelial receptors and induce antibodies response during natural infections. In this chapter, we describe a competition ELISA that is an assay for antibodies neutralizing binding of PfEMP1 proteins to their cognate receptor.

Original languageEnglish
Title of host publicationMalaria Immunology : Targeting the Surface of Infected Erythrocytes
Number of pages7
Volume2470
PublisherHumana Press
Publication date2022
Pages485-491
ISBN (Electronic)978-1-0716-2189-9
DOIs
Publication statusPublished - 2022
SeriesMethods in molecular biology (Clifton, N.J.)
ISSN1064-3745

Bibliographical note

© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

    Research areas

  • Antibodies, Protozoan, Antigens, Protozoan, Enzyme-Linked Immunosorbent Assay, Erythrocytes/metabolism, Humans, Malaria, Falciparum, Plasmodium falciparum/metabolism, Protein Binding, Protozoan Proteins/metabolism

ID: 320649348