Afucosylated Plasmodium falciparum-specific IgG is induced by infection but not by subunit vaccination

Research output: Contribution to journal › Journal article › Research › peer-review

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Afucosylated Plasmodium falciparum-specific IgG is induced by infection but not by subunit vaccination. / Larsen, Mads Delbo; Lopez-Perez, Mary; Dickson, Emmanuel Kakra; Ampomah, Paulina; Tuikue Ndam, Nicaise; Nouta, Jan; Koeleman, Carolien A M; Ederveen, Agnes L Hipgrave; Mordmüller, Benjamin; Salanti, Ali; Nielsen, Morten Agertoug; Massougbodji, Achille; van der Schoot, C Ellen; Ofori, Michael F; Wuhrer, Manfred; Hviid, Lars; Vidarsson, Gestur.

In: Nature Communications, Vol. 12, 5838, 2021.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Larsen, MD, Lopez-Perez, M, Dickson, EK, Ampomah, P, Tuikue Ndam, N, Nouta, J, Koeleman, CAM, Ederveen, ALH, Mordmüller, B, Salanti, A, Nielsen, MA, Massougbodji, A, van der Schoot, CE, Ofori, MF, Wuhrer, M, Hviid, L & Vidarsson, G 2021, 'Afucosylated Plasmodium falciparum-specific IgG is induced by infection but not by subunit vaccination', Nature Communications, vol. 12, 5838. https://doi.org/10.1038/s41467-021-26118-w

APA

Larsen, M. D., Lopez-Perez, M., Dickson, E. K., Ampomah, P., Tuikue Ndam, N., Nouta, J., Koeleman, C. A. M., Ederveen, A. L. H., Mordmüller, B., Salanti, A., Nielsen, M. A., Massougbodji, A., van der Schoot, C. E., Ofori, M. F., Wuhrer, M., Hviid, L., & Vidarsson, G. (2021). Afucosylated Plasmodium falciparum-specific IgG is induced by infection but not by subunit vaccination. Nature Communications, 12, [5838]. https://doi.org/10.1038/s41467-021-26118-w

Vancouver

Larsen MD, Lopez-Perez M, Dickson EK, Ampomah P, Tuikue Ndam N, Nouta J et al. Afucosylated Plasmodium falciparum-specific IgG is induced by infection but not by subunit vaccination. Nature Communications. 2021;12. 5838. https://doi.org/10.1038/s41467-021-26118-w

Author

Larsen, Mads Delbo ; Lopez-Perez, Mary ; Dickson, Emmanuel Kakra ; Ampomah, Paulina ; Tuikue Ndam, Nicaise ; Nouta, Jan ; Koeleman, Carolien A M ; Ederveen, Agnes L Hipgrave ; Mordmüller, Benjamin ; Salanti, Ali ; Nielsen, Morten Agertoug ; Massougbodji, Achille ; van der Schoot, C Ellen ; Ofori, Michael F ; Wuhrer, Manfred ; Hviid, Lars ; Vidarsson, Gestur. / Afucosylated Plasmodium falciparum-specific IgG is induced by infection but not by subunit vaccination. In: Nature Communications. 2021 ; Vol. 12.

Bibtex

@article{e7c69d7c584445f2b64ee6bd9fe62768,
title = "Afucosylated Plasmodium falciparum-specific IgG is induced by infection but not by subunit vaccination",
abstract = "Plasmodium falciparum erythrocyte membrane protein 1 (PfEMP1) family members mediate receptor- and tissue-specific sequestration of infected erythrocytes (IEs) in malaria. Antibody responses are a central component of naturally acquired malaria immunity. PfEMP1-specific IgG likely protects by inhibiting IE sequestration and through IgG-Fc Receptor (FcγR) mediated phagocytosis and killing of antibody-opsonized IEs. The affinity of afucosylated IgG to FcγRIIIa is up to 40-fold higher than fucosylated IgG, resulting in enhanced antibody-dependent cellular cytotoxicity. Most IgG in plasma is fully fucosylated, but afucosylated IgG is elicited in response to enveloped viruses and to paternal alloantigens during pregnancy. Here we show that naturally acquired PfEMP1-specific IgG is strongly afucosylated in a stable and exposure-dependent manner, and efficiently induces FcγRIIIa-dependent natural killer (NK) cell degranulation. In contrast, immunization with a subunit PfEMP1 (VAR2CSA) vaccine results in fully fucosylated specific IgG. These results have implications for understanding protective natural- and vaccine-induced immunity to malaria.",
author = "Larsen, {Mads Delbo} and Mary Lopez-Perez and Dickson, {Emmanuel Kakra} and Paulina Ampomah and {Tuikue Ndam}, Nicaise and Jan Nouta and Koeleman, {Carolien A M} and Ederveen, {Agnes L Hipgrave} and Benjamin Mordm{\"u}ller and Ali Salanti and Nielsen, {Morten Agertoug} and Achille Massougbodji and {van der Schoot}, {C Ellen} and Ofori, {Michael F} and Manfred Wuhrer and Lars Hviid and Gestur Vidarsson",
note = "{\textcopyright} 2021. The Author(s).",
year = "2021",
doi = "10.1038/s41467-021-26118-w",
language = "English",
volume = "12",
journal = "Nature Communications",
issn = "2041-1723",
publisher = "nature publishing group",

}

RIS

TY - JOUR

T1 - Afucosylated Plasmodium falciparum-specific IgG is induced by infection but not by subunit vaccination

AU - Larsen, Mads Delbo

AU - Lopez-Perez, Mary

AU - Dickson, Emmanuel Kakra

AU - Ampomah, Paulina

AU - Tuikue Ndam, Nicaise

AU - Nouta, Jan

AU - Koeleman, Carolien A M

AU - Ederveen, Agnes L Hipgrave

AU - Mordmüller, Benjamin

AU - Salanti, Ali

AU - Nielsen, Morten Agertoug

AU - Massougbodji, Achille

AU - van der Schoot, C Ellen

AU - Ofori, Michael F

AU - Wuhrer, Manfred

AU - Hviid, Lars

AU - Vidarsson, Gestur

N1 - © 2021. The Author(s).

PY - 2021

Y1 - 2021

N2 - Plasmodium falciparum erythrocyte membrane protein 1 (PfEMP1) family members mediate receptor- and tissue-specific sequestration of infected erythrocytes (IEs) in malaria. Antibody responses are a central component of naturally acquired malaria immunity. PfEMP1-specific IgG likely protects by inhibiting IE sequestration and through IgG-Fc Receptor (FcγR) mediated phagocytosis and killing of antibody-opsonized IEs. The affinity of afucosylated IgG to FcγRIIIa is up to 40-fold higher than fucosylated IgG, resulting in enhanced antibody-dependent cellular cytotoxicity. Most IgG in plasma is fully fucosylated, but afucosylated IgG is elicited in response to enveloped viruses and to paternal alloantigens during pregnancy. Here we show that naturally acquired PfEMP1-specific IgG is strongly afucosylated in a stable and exposure-dependent manner, and efficiently induces FcγRIIIa-dependent natural killer (NK) cell degranulation. In contrast, immunization with a subunit PfEMP1 (VAR2CSA) vaccine results in fully fucosylated specific IgG. These results have implications for understanding protective natural- and vaccine-induced immunity to malaria.

AB - Plasmodium falciparum erythrocyte membrane protein 1 (PfEMP1) family members mediate receptor- and tissue-specific sequestration of infected erythrocytes (IEs) in malaria. Antibody responses are a central component of naturally acquired malaria immunity. PfEMP1-specific IgG likely protects by inhibiting IE sequestration and through IgG-Fc Receptor (FcγR) mediated phagocytosis and killing of antibody-opsonized IEs. The affinity of afucosylated IgG to FcγRIIIa is up to 40-fold higher than fucosylated IgG, resulting in enhanced antibody-dependent cellular cytotoxicity. Most IgG in plasma is fully fucosylated, but afucosylated IgG is elicited in response to enveloped viruses and to paternal alloantigens during pregnancy. Here we show that naturally acquired PfEMP1-specific IgG is strongly afucosylated in a stable and exposure-dependent manner, and efficiently induces FcγRIIIa-dependent natural killer (NK) cell degranulation. In contrast, immunization with a subunit PfEMP1 (VAR2CSA) vaccine results in fully fucosylated specific IgG. These results have implications for understanding protective natural- and vaccine-induced immunity to malaria.

U2 - 10.1038/s41467-021-26118-w

DO - 10.1038/s41467-021-26118-w

M3 - Journal article

C2 - 34611164

VL - 12

JO - Nature Communications

JF - Nature Communications

SN - 2041-1723

M1 - 5838

ER -

ID: 281284752