A synthetic TLR4 agonist formulated in an emulsion enhances humoral and Type 1 cellular immune responses against GMZ2 - A GLURP-MSP3 fusion protein malaria vaccine candidate

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

A synthetic TLR4 agonist formulated in an emulsion enhances humoral and Type 1 cellular immune responses against GMZ2 - A GLURP-MSP3 fusion protein malaria vaccine candidate. / Lousada-Dietrich, Susana; Jogdand, Prajakta S; Jepsen, Søren; Valadão Vaz dos Santos Pinto, Vera Manuel; Ditlev, Sisse B; Christiansen, Michael; Larsen, Severin Olesen; Fox, Christopher B; Raman, Vanitha S; Howard, Randall F; Vedvick, Thomas S; Ireton, Gregory; Carter, Darrick; Reed, Steven G; Theisen, Michael.

In: Vaccine, Vol. 29, No. 17, 12.04.2011, p. 3284-92.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Lousada-Dietrich, S, Jogdand, PS, Jepsen, S, Valadão Vaz dos Santos Pinto, VM, Ditlev, SB, Christiansen, M, Larsen, SO, Fox, CB, Raman, VS, Howard, RF, Vedvick, TS, Ireton, G, Carter, D, Reed, SG & Theisen, M 2011, 'A synthetic TLR4 agonist formulated in an emulsion enhances humoral and Type 1 cellular immune responses against GMZ2 - A GLURP-MSP3 fusion protein malaria vaccine candidate', Vaccine, vol. 29, no. 17, pp. 3284-92. https://doi.org/10.1016/j.vaccine.2011.02.022

APA

Lousada-Dietrich, S., Jogdand, P. S., Jepsen, S., Valadão Vaz dos Santos Pinto, V. M., Ditlev, S. B., Christiansen, M., Larsen, S. O., Fox, C. B., Raman, V. S., Howard, R. F., Vedvick, T. S., Ireton, G., Carter, D., Reed, S. G., & Theisen, M. (2011). A synthetic TLR4 agonist formulated in an emulsion enhances humoral and Type 1 cellular immune responses against GMZ2 - A GLURP-MSP3 fusion protein malaria vaccine candidate. Vaccine, 29(17), 3284-92. https://doi.org/10.1016/j.vaccine.2011.02.022

Vancouver

Lousada-Dietrich S, Jogdand PS, Jepsen S, Valadão Vaz dos Santos Pinto VM, Ditlev SB, Christiansen M et al. A synthetic TLR4 agonist formulated in an emulsion enhances humoral and Type 1 cellular immune responses against GMZ2 - A GLURP-MSP3 fusion protein malaria vaccine candidate. Vaccine. 2011 Apr 12;29(17):3284-92. https://doi.org/10.1016/j.vaccine.2011.02.022

Author

Lousada-Dietrich, Susana ; Jogdand, Prajakta S ; Jepsen, Søren ; Valadão Vaz dos Santos Pinto, Vera Manuel ; Ditlev, Sisse B ; Christiansen, Michael ; Larsen, Severin Olesen ; Fox, Christopher B ; Raman, Vanitha S ; Howard, Randall F ; Vedvick, Thomas S ; Ireton, Gregory ; Carter, Darrick ; Reed, Steven G ; Theisen, Michael. / A synthetic TLR4 agonist formulated in an emulsion enhances humoral and Type 1 cellular immune responses against GMZ2 - A GLURP-MSP3 fusion protein malaria vaccine candidate. In: Vaccine. 2011 ; Vol. 29, No. 17. pp. 3284-92.

Bibtex

@article{f8ec19619ca64a05bb68e94bd491c726,
title = "A synthetic TLR4 agonist formulated in an emulsion enhances humoral and Type 1 cellular immune responses against GMZ2 - A GLURP-MSP3 fusion protein malaria vaccine candidate",
abstract = "GMZ2 adjuvanted by aluminum hydroxide is a candidate malaria vaccine that has successfully passed phase 1 clinical testing in adult German and Gabonese volunteers and Gabonese children under five. Here we report a preclinical study screening a series of adjuvant vehicles and Toll-like receptor (TLR) agonists in CB6F1 mice to identify an improved formulation of GMZ2 suitable for further human clinical studies. GMZ2 formulated in an oil-in-water emulsion plus the synthetic TLR4 agonist GLA elicits the highest (a) vaccine-specific IgG2a and total IgG titers, (b) parasite-specific IFA titers, (c) levels of Type 1 cytokine responses (IFN-¿), and (d) number of long-lived-plasma cells (LLPC) secreting antibodies against both the GMZ2 fusion and its two components. Thus, GLA helps to elicit a vaccine-specific Type 1 antibody profile together with high levels of LLPC, both of which are thought to be essential for the development of long-term protective immunity against clinical malaria.",
author = "Susana Lousada-Dietrich and Jogdand, {Prajakta S} and S{\o}ren Jepsen and {Valad{\~a}o Vaz dos Santos Pinto}, {Vera Manuel} and Ditlev, {Sisse B} and Michael Christiansen and Larsen, {Severin Olesen} and Fox, {Christopher B} and Raman, {Vanitha S} and Howard, {Randall F} and Vedvick, {Thomas S} and Gregory Ireton and Darrick Carter and Reed, {Steven G} and Michael Theisen",
note = "Copyright {\textcopyright} 2011 Elsevier Ltd. All rights reserved.",
year = "2011",
month = apr,
day = "12",
doi = "10.1016/j.vaccine.2011.02.022",
language = "English",
volume = "29",
pages = "3284--92",
journal = "Vaccine",
issn = "0264-410X",
publisher = "Elsevier",
number = "17",

}

RIS

TY - JOUR

T1 - A synthetic TLR4 agonist formulated in an emulsion enhances humoral and Type 1 cellular immune responses against GMZ2 - A GLURP-MSP3 fusion protein malaria vaccine candidate

AU - Lousada-Dietrich, Susana

AU - Jogdand, Prajakta S

AU - Jepsen, Søren

AU - Valadão Vaz dos Santos Pinto, Vera Manuel

AU - Ditlev, Sisse B

AU - Christiansen, Michael

AU - Larsen, Severin Olesen

AU - Fox, Christopher B

AU - Raman, Vanitha S

AU - Howard, Randall F

AU - Vedvick, Thomas S

AU - Ireton, Gregory

AU - Carter, Darrick

AU - Reed, Steven G

AU - Theisen, Michael

N1 - Copyright © 2011 Elsevier Ltd. All rights reserved.

PY - 2011/4/12

Y1 - 2011/4/12

N2 - GMZ2 adjuvanted by aluminum hydroxide is a candidate malaria vaccine that has successfully passed phase 1 clinical testing in adult German and Gabonese volunteers and Gabonese children under five. Here we report a preclinical study screening a series of adjuvant vehicles and Toll-like receptor (TLR) agonists in CB6F1 mice to identify an improved formulation of GMZ2 suitable for further human clinical studies. GMZ2 formulated in an oil-in-water emulsion plus the synthetic TLR4 agonist GLA elicits the highest (a) vaccine-specific IgG2a and total IgG titers, (b) parasite-specific IFA titers, (c) levels of Type 1 cytokine responses (IFN-¿), and (d) number of long-lived-plasma cells (LLPC) secreting antibodies against both the GMZ2 fusion and its two components. Thus, GLA helps to elicit a vaccine-specific Type 1 antibody profile together with high levels of LLPC, both of which are thought to be essential for the development of long-term protective immunity against clinical malaria.

AB - GMZ2 adjuvanted by aluminum hydroxide is a candidate malaria vaccine that has successfully passed phase 1 clinical testing in adult German and Gabonese volunteers and Gabonese children under five. Here we report a preclinical study screening a series of adjuvant vehicles and Toll-like receptor (TLR) agonists in CB6F1 mice to identify an improved formulation of GMZ2 suitable for further human clinical studies. GMZ2 formulated in an oil-in-water emulsion plus the synthetic TLR4 agonist GLA elicits the highest (a) vaccine-specific IgG2a and total IgG titers, (b) parasite-specific IFA titers, (c) levels of Type 1 cytokine responses (IFN-¿), and (d) number of long-lived-plasma cells (LLPC) secreting antibodies against both the GMZ2 fusion and its two components. Thus, GLA helps to elicit a vaccine-specific Type 1 antibody profile together with high levels of LLPC, both of which are thought to be essential for the development of long-term protective immunity against clinical malaria.

U2 - 10.1016/j.vaccine.2011.02.022

DO - 10.1016/j.vaccine.2011.02.022

M3 - Journal article

C2 - 21349366

VL - 29

SP - 3284

EP - 3292

JO - Vaccine

JF - Vaccine

SN - 0264-410X

IS - 17

ER -

ID: 33325738