3D organoid assay of the impact of infected erythrocyte adhesion on the blood-brain barrier

Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review

Standard

3D organoid assay of the impact of infected erythrocyte adhesion on the blood-brain barrier. / Adams, Yvonne; Jensen, Anja Ramstedt.

Malaria Immunology: Targeting the Surface of Infected Erythrocytes. Vol. 2470 Humana Press, 2022. p. 587-599 (Methods in molecular biology (Clifton, N.J.)).

Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review

Harvard

Adams, Y & Jensen, AR 2022, 3D organoid assay of the impact of infected erythrocyte adhesion on the blood-brain barrier. in Malaria Immunology: Targeting the Surface of Infected Erythrocytes. vol. 2470, Humana Press, Methods in molecular biology (Clifton, N.J.), pp. 587-599. https://doi.org/10.1007/978-1-0716-2189-9_44

APA

Adams, Y., & Jensen, A. R. (2022). 3D organoid assay of the impact of infected erythrocyte adhesion on the blood-brain barrier. In Malaria Immunology: Targeting the Surface of Infected Erythrocytes (Vol. 2470, pp. 587-599). Humana Press. Methods in molecular biology (Clifton, N.J.) https://doi.org/10.1007/978-1-0716-2189-9_44

Vancouver

Adams Y, Jensen AR. 3D organoid assay of the impact of infected erythrocyte adhesion on the blood-brain barrier. In Malaria Immunology: Targeting the Surface of Infected Erythrocytes. Vol. 2470. Humana Press. 2022. p. 587-599. (Methods in molecular biology (Clifton, N.J.)). https://doi.org/10.1007/978-1-0716-2189-9_44

Author

Adams, Yvonne ; Jensen, Anja Ramstedt. / 3D organoid assay of the impact of infected erythrocyte adhesion on the blood-brain barrier. Malaria Immunology: Targeting the Surface of Infected Erythrocytes. Vol. 2470 Humana Press, 2022. pp. 587-599 (Methods in molecular biology (Clifton, N.J.)).

Bibtex

@inbook{835df23cac434f1eb8f900d9ca134cbf,
title = "3D organoid assay of the impact of infected erythrocyte adhesion on the blood-brain barrier",
abstract = "Mass sequestration of Plasmodium falciparum parasites in the brain microvasculature can lead to cerebral malaria (CM), characterized by inflammation, vessel occlusion, and brain swelling. To date, only single-cell-type, monolayer assays have been used to investigate the effect of infected erythrocytes (IEs) on the human blood-brain barrier (BBB) and the underlying parenchyma. Here we present a human-derived 3D model of the BBB comprised of endothelial cells, pericytes, and astrocytes in direct contact with each other. The organoids readily self-assemble and can easily be grown in 96-well plates, allowing for high-throughput analysis. These organoids allow for the assessment of parasite adhesion, and analysis of barrier function, and gross morphological changes in response to parasite exposure.",
keywords = "Blood-Brain Barrier/metabolism, Cell Adhesion/physiology, Endothelial Cells/metabolism, Erythrocytes/metabolism, Humans, Malaria, Falciparum/parasitology, Organoids/metabolism, Plasmodium falciparum/metabolism, Protozoan Proteins/metabolism",
author = "Yvonne Adams and Jensen, {Anja Ramstedt}",
note = "{\textcopyright} 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2022",
doi = "10.1007/978-1-0716-2189-9_44",
language = "English",
volume = "2470",
series = "Methods in molecular biology (Clifton, N.J.)",
publisher = "Humana Press",
pages = "587--599",
booktitle = "Malaria Immunology",
address = "United States",

}

RIS

TY - CHAP

T1 - 3D organoid assay of the impact of infected erythrocyte adhesion on the blood-brain barrier

AU - Adams, Yvonne

AU - Jensen, Anja Ramstedt

N1 - © 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

PY - 2022

Y1 - 2022

N2 - Mass sequestration of Plasmodium falciparum parasites in the brain microvasculature can lead to cerebral malaria (CM), characterized by inflammation, vessel occlusion, and brain swelling. To date, only single-cell-type, monolayer assays have been used to investigate the effect of infected erythrocytes (IEs) on the human blood-brain barrier (BBB) and the underlying parenchyma. Here we present a human-derived 3D model of the BBB comprised of endothelial cells, pericytes, and astrocytes in direct contact with each other. The organoids readily self-assemble and can easily be grown in 96-well plates, allowing for high-throughput analysis. These organoids allow for the assessment of parasite adhesion, and analysis of barrier function, and gross morphological changes in response to parasite exposure.

AB - Mass sequestration of Plasmodium falciparum parasites in the brain microvasculature can lead to cerebral malaria (CM), characterized by inflammation, vessel occlusion, and brain swelling. To date, only single-cell-type, monolayer assays have been used to investigate the effect of infected erythrocytes (IEs) on the human blood-brain barrier (BBB) and the underlying parenchyma. Here we present a human-derived 3D model of the BBB comprised of endothelial cells, pericytes, and astrocytes in direct contact with each other. The organoids readily self-assemble and can easily be grown in 96-well plates, allowing for high-throughput analysis. These organoids allow for the assessment of parasite adhesion, and analysis of barrier function, and gross morphological changes in response to parasite exposure.

KW - Blood-Brain Barrier/metabolism

KW - Cell Adhesion/physiology

KW - Endothelial Cells/metabolism

KW - Erythrocytes/metabolism

KW - Humans

KW - Malaria, Falciparum/parasitology

KW - Organoids/metabolism

KW - Plasmodium falciparum/metabolism

KW - Protozoan Proteins/metabolism

U2 - 10.1007/978-1-0716-2189-9_44

DO - 10.1007/978-1-0716-2189-9_44

M3 - Book chapter

C2 - 35881376

VL - 2470

T3 - Methods in molecular biology (Clifton, N.J.)

SP - 587

EP - 599

BT - Malaria Immunology

PB - Humana Press

ER -

ID: 320648836